Anticancer effects of Erzhimaoling decoction in high-grade serous ovarian cancer in vitro and in vivo DOI Creative Commons
Yang Li, Jingfang Liu, Jiejie Zhang

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: Aug. 5, 2024

High-grade serous ovarian cancer (HGSOC) is a common gynecologic malignancy with poor prognosis. The traditional Chinese medicine formula Erzhimaoling decoction (EZMLD) has anticancer potential. This study aims to elucidate the effects of EZMLD on HGSOC in vitro and vivo. EZMLD-containing serum was prepared from Sprague-Dawley rats for treating SKOV3 cells at varying concentrations 24 h 48 determine IC50. Concentrations 0%, 5%, 10% were chosen subsequent experiments. roles METTL3 METTL14 explored by overexpressing these genes combining METTL3/14 knockdown. Investigations focused cell viability apoptosis, apoptosis-related protein expression, KRT8 mRNA m6A modification. For vivo studies, 36 BALB/c nude mice divided into six groups involving (6.75, 13.5, 27 g/kg) or knockdowns, daily gavage two weeks. In vitro, had IC50 values 8.29% 5.95% cells. decreased increased apoptosis. upregulated FAS-mediated apoptosis proteins, while downregulating Keratin 8 (KRT8). methylation. overexpression reduced knockdown mitigated EZMLD's effects. vivo, suppressed xenografts growth, causing significant modulating expression. therapeutic potential may be considered other types. Future research explore its broader beyond

Language: Английский

FoxO1 promotes ovarian cancer by increasing transcription and METTL14‐mediated m6A modification of SMC4 DOI Creative Commons
Liping Tan,

Shuangan Wang,

Shijia Huang

et al.

Cancer Science, Journal Year: 2024, Volume and Issue: 115(4), P. 1224 - 1240

Published: Feb. 25, 2024

The transcription factor forkhead box protein O1 (FoxO1) is closely related to the occurrence and development of ovarian cancer (OC), however its role molecular mechanisms remain unclear. Herein, we found that FoxO1 was highly expressed in clinical samples OC patients significantly correlated with poor prognosis. knockdown inhibited proliferation cells vitro vivo. ChIP-seq combined GEPIA2 Kaplan-Meier database analysis showed structural maintenance chromosome 4 (SMC4) a downstream target FoxO1, promotes SMC4 by binding -1400/-1390 bp promoter. high expression blocked tumor inhibition effect knockdown. Furtherly, increased mRNA abundance transcriptionally activating methyltransferase-like 14 (METTL14) increasing m

Language: Английский

Citations

6
Investigation of the mechanism by which FOXJ2 inhibits proliferation, metastasis and cell cycle progression of ovarian cancer cells through the PI3K/AKT signaling pathway DOI Creative Commons
Liyuan Wang, Han He,

Ruifang Zhai

et al.

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: March 4, 2025

As one member of the Forkhead Box transcription factor, J2 (FOXJ2) is involved in diverse cancers. At present, specific role and mechanism FOXJ2 ovarian cancer (OC) have not been fully addressed, which allows us to fill blank. Accordingly, expression OC cells epithelial was quantified via real-time qPCR. Following transfection, cell counting kit-8, Transwell, wound healing flow cytometry assays were performed measure proliferation, metastasis, apoptosis cycle A2780 HEY. Further, qPCR Western blotting both employed for quantification on levels as well phosphoinositide 3-kinase (PI3K) protein kinase B (AKT) (in unphosphorylated phosphorylated forms). Based results, highly-expressed (P < 0.05). Silencing resulted attenuated reduced numbers migrating invading cells, decreased apoptotic capacity, arrest G1/S phase In addition, knockdown caused downward trend phosphorylation level PI3K AKT The silencing could repress growth metastasis potentials cause vitro, possibly achieved targeting PI3K/AKT pathway.

Language: Английский

Citations

0
METTL14 mediates nerve growth factor-stimulated testosterone synthesis in porcine theca cells DOI
Yuxin Luo, Yun Zhao, Boqi Zhang

et al.

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 111(3), P. 655 - 666

Published: June 28, 2024

Ovarian theca cells produce testosterone, which acts as a vital precursor substance for synthesizing estrogens during follicular development. Nerve growth factor (NGF) has been shown to participate in reproductive physiology, specifically development and ovulation. There is currently no available data on the impact of NGF testosterone synthesis porcine cells. Furthermore, m6A modification most common internal eukaryotic mRNAs that are closely associated with female gametogenesis, follicle development, ovulation, other related processes. It also uncertain whether three main enzymes m6A, such Writers, Erasers, Readers, play role this process. The present study, an vitro culture model, investigated effect Writers-METTL14 was found activates PI3K/AKT signaling pathway through METTL14, regulates This study will help further elucidate mechanisms by provide new therapeutic targets ovary-related diseases animals. Summary Sentence

Language: Английский

Citations

1
N6‐Methyladenosine Modification on the Function of Female Reproductive Development and Related Diseases DOI Creative Commons
Xiangrong Cui, Huihui Li,

Xia Huang

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(12)

Published: Dec. 1, 2024

N6-methyladenosine (m6A) modification is a widespread and reversible epigenetic alteration in eukaryotic mRNA, playing pivotal role various biological functions. Its significance female reproductive development associated diseases has recently become focal point of research.

Language: Английский

Citations

1
Shining a Spotlight on RNA m6A Modifications and Potential Targeted Immunotherapy in Ovarian Cancer DOI Creative Commons

Huishen Pang,

Weiping Zheng,

Qiyun Lu

et al.

Clinical and Experimental Obstetrics & Gynecology, Journal Year: 2024, Volume and Issue: 51(12)

Published: Dec. 23, 2024

Objective: Ovarian cancer is one of the major gynecologic malignancies worldwide. Evidence indicates that N6-methyladenosine (m6A) modification strongly related to immunity. This review aims demonstrate role and function m6A in ovarian tumorigenesis its potential targeted immunotherapy. Mechanism: Searching literature for articles Conducting a comprehensive analysis summarizing content logically. Findings Brief: In this review, we summarized latest research on regulators Firstly, found underlying molecular mechanism (writers, erasers readers) development progress cancer. Then, discovered play pivotal immune responses efficacy, serving as therapeutic targets Additionally, long noncoding RNAs (lncRNAs) have strong impact survival Finally, deeply analyzing genes are needed improve immunotherapy Conclusions: has carcinogenesis

Language: Английский

Citations

0
Anticancer effects of Erzhimaoling decoction in high-grade serous ovarian cancer in vitro and in vivo DOI Creative Commons
Yang Li, Jingfang Liu, Jiejie Zhang

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: Aug. 5, 2024

High-grade serous ovarian cancer (HGSOC) is a common gynecologic malignancy with poor prognosis. The traditional Chinese medicine formula Erzhimaoling decoction (EZMLD) has anticancer potential. This study aims to elucidate the effects of EZMLD on HGSOC in vitro and vivo. EZMLD-containing serum was prepared from Sprague-Dawley rats for treating SKOV3 cells at varying concentrations 24 h 48 determine IC50. Concentrations 0%, 5%, 10% were chosen subsequent experiments. roles METTL3 METTL14 explored by overexpressing these genes combining METTL3/14 knockdown. Investigations focused cell viability apoptosis, apoptosis-related protein expression, KRT8 mRNA m6A modification. For vivo studies, 36 BALB/c nude mice divided into six groups involving (6.75, 13.5, 27 g/kg) or knockdowns, daily gavage two weeks. In vitro, had IC50 values 8.29% 5.95% cells. decreased increased apoptosis. upregulated FAS-mediated apoptosis proteins, while downregulating Keratin 8 (KRT8). methylation. overexpression reduced knockdown mitigated EZMLD's effects. vivo, suppressed xenografts growth, causing significant modulating expression. therapeutic potential may be considered other types. Future research explore its broader beyond

Language: Английский

Citations

0