Unveiling purine metabolism dysregulation orchestrated immunosuppression in advanced pancreatic cancer and concentrating on the central role of NT5E DOI Creative Commons
Junqian Zhang,

Xiaobo Zhang,

R. Wu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

The dismal efficacy of immunotherapy for Pancreatic cancer (PC) can be predominantly ascribed to its distinctive cold-tumor properties. by-products purine metabolic reprogramming are extensively engaged in tumor immune modulation, influencing the functions and recruitment cells molding an microenvironment that is propitious growth. We harnessed single-cell transcriptomics spatial concurrently analyze metabolism (PM) features PC microenvironment. quantitatively appraised PM traits diverse cell subsets via scoring algorithms such as AUCell Ucell. Moreover, development cell-cell interaction analysis elucidated alterations TME induced by dysregulation. Additionally, we defined disorder characteristics patients utilized this assess phenotypes prognoses patient population. Also, identified crucial intermediate genes impact establishment immunosuppressive environment within PC, validated them through sectioning co-culture experiments. Multi - dimensional transcriptome data unique heterogeneity microenvironment, which manifested fibroblasts demonstrating higher scores TME. Cellchat revealed malignant with elevated expression were concomitantly associated frequent interactions CAFs well high ligand-receptor pairs transcription factors. Spatial further corroborated finding. Furthermore, newly constructed criteria indicated levels a lack response Finally, study singular role NT5E immunosuppression resulting from PC. CCK8 invasion experiments following model demonstrated intervention targeting could reverse augmented malignancy co-cultured CAFs. potentially key target reversing "stiff-cancer" This demonstrates disorders impinge upon exacerbate engendered progression fibrosis. Therapeutic strategies or may offer ray hope advanced PDAC.

Language: Английский

Cancer-associated fibroblasts in breast cancer in the single-cell era: Opportunities and challenges DOI

Jingtong Yang,

Benkai Xin,

Xiaoyu Wang

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2025, Volume and Issue: unknown, P. 189291 - 189291

Published: Feb. 1, 2025

Language: Английский

Citations

0
Advances and future directions in radiopharmaceutical delivery for cancer treatment DOI
Sikander Ailawadhi,

Deanna Pafundi,

Jennifer Peterson

et al.

Expert Review of Anticancer Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Targeted radiopharmaceutical therapies (RPTs) have emerged as a promising approach for the precise treatment of various cancers. Delivering ionizing radiation directly to cancer cells while sparing surrounding healthy tissue, radiopharmaceuticals offer enhanced efficacy and reduced toxicity compared conventional external beam therapy (i.e. photons electrons). In current era personalized care, appropriate choice RPTs clinical condition specific patient's care needs be better understood. Several available RPT agents with their respective applicability along rapidly ongoing research in this field now given ability lend themselves medicine focus. This review provides an overview recent advancements RPT, including nuclide selection development, molecular targeting strategies, applications. We discuss underlying principles, challenges, opportunities future development. Furthermore, we explore emerging technologies directions field, highlighting potential impact on care.

Language: Английский

Citations

0
Mechanistic role of stromal cancer-associated fibroblasts in tumorigenesis and brain metastasis: highlighting drug resistance and targeted therapy DOI
Md Rashedunnabi Akanda,

Umme Lubaba,

Md Khalesur Rahman

et al.

Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: unknown, P. 155918 - 155918

Published: March 1, 2025

Language: Английский

Citations

0
Unveiling purine metabolism dysregulation orchestrated immunosuppression in advanced pancreatic cancer and concentrating on the central role of NT5E DOI Creative Commons
Junqian Zhang,

Xiaobo Zhang,

R. Wu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

The dismal efficacy of immunotherapy for Pancreatic cancer (PC) can be predominantly ascribed to its distinctive cold-tumor properties. by-products purine metabolic reprogramming are extensively engaged in tumor immune modulation, influencing the functions and recruitment cells molding an microenvironment that is propitious growth. We harnessed single-cell transcriptomics spatial concurrently analyze metabolism (PM) features PC microenvironment. quantitatively appraised PM traits diverse cell subsets via scoring algorithms such as AUCell Ucell. Moreover, development cell-cell interaction analysis elucidated alterations TME induced by dysregulation. Additionally, we defined disorder characteristics patients utilized this assess phenotypes prognoses patient population. Also, identified crucial intermediate genes impact establishment immunosuppressive environment within PC, validated them through sectioning co-culture experiments. Multi - dimensional transcriptome data unique heterogeneity microenvironment, which manifested fibroblasts demonstrating higher scores TME. Cellchat revealed malignant with elevated expression were concomitantly associated frequent interactions CAFs well high ligand-receptor pairs transcription factors. Spatial further corroborated finding. Furthermore, newly constructed criteria indicated levels a lack response Finally, study singular role NT5E immunosuppression resulting from PC. CCK8 invasion experiments following model demonstrated intervention targeting could reverse augmented malignancy co-cultured CAFs. potentially key target reversing "stiff-cancer" This demonstrates disorders impinge upon exacerbate engendered progression fibrosis. Therapeutic strategies or may offer ray hope advanced PDAC.

Language: Английский

Citations

0