Identification and Diagnosis of Possible Mutations in Human P-53 Lymphocytes DOI Creative Commons
Delia Nica-Badea, Tataru Tiberiu,

Marinas Marius Cristian

et al.

Pharmacophore, Journal Year: 2023, Volume and Issue: 14(6), P. 52 - 58

Published: Jan. 1, 2023

This study looked at a few confirmed cases of chronic lymphocytic leukemia (CLL) that may have had mutations in the human B lymphocyte genome's P-53 gene.The ELISA technique was used to determine frequency p-53 protein expression twenty CLL patients undergoing evaluation stages II-III / IV.In 17 20 cases, average amounts proteins were found be 16.76 μg/dl, with probability index p = 0.034 and CV 0.5%.It discovered as illness progressed, proportion positive isoform rose above normal.The percentage 15% ± 2 1-2 100% 3-4.Recent studies shown inhibits kinase B, AMPK protein, mTOR complex, hence mediating genes trigger autophagy stimulating autophagy.One potential use protein's role cancer cell is development novel anti-cancer medicinal approach.When patient develops resistance initial line treatment, approach has an invaluable prognostic tool for CLL, enabling administration customized medication.

Language: Английский

Targeting the key players of phenotypic plasticity in cancer cells by phytochemicals DOI
Sajad Fakhri, Seyed Zachariah Moradi, Fatemeh Abbaszadeh

et al.

Cancer and Metastasis Reviews, Journal Year: 2024, Volume and Issue: 43(1), P. 261 - 292

Published: Jan. 3, 2024

Language: Английский

Citations

1
Berberine potentiates liver inflammation and fibrosis in the PI*Z hAAT transgenic murine model DOI Creative Commons
Yuanqing Lu,

Naweed Mohammad,

Jungnam Lee

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(9), P. e0310524 - e0310524

Published: Sept. 19, 2024

Background Alpha-1 antitrypsin deficiency (AATD) is an inherited disease, the common variant caused by a Pi*Z mutation in SERPINA1 gene. AAT increases risk of pulmonary emphysema and liver disease. Berberine (BBR) nature dietary supplement herbal remedy. Emerging evidence revealed that BBR has remarkable liver-protective properties against various diseases. In present study, we investigated therapeutic effects toxicities hepatocytes transgenic mice. Methods Huh7.5 Huh7.5Z (which carries mutation) cells were treated with different concentrations for 48 hours. MTT was performed cell viability assay. Intracellular levels evaluated western blot. vivo studies carried out wild type, native phenotype (Pi*M), Mice 50 mg/kg/day or solvent only oral administration 30 days. Western blot histopathological examinations to evaluate benefits toxicity BBR. Results reduced intracellular cells, meanwhile, no Pi*Z-specific observed. However, did not reduce load but significantly potentiated inflammation fibrosis accompanying activation unfolded protein response mTOR mice, type Pi*M Conclusions exacerbated specifically This adverse effect may be associated mTOR. study implicates should avoided AATD patients.

Language: Английский

Citations

1
Berberine: A multifaceted agent for lung cancer treatment-from molecular insight to clinical applications DOI
Xiaodan Xu,

He Yuanyuan,

Jungang Liu

et al.

Gene, Journal Year: 2024, Volume and Issue: unknown, P. 149021 - 149021

Published: Oct. 1, 2024

Language: Английский

Citations

1
Definition of the Neurotoxicity-Associated Metabolic Signature Triggered by Berberine and Other Respiratory Chain Inhibitors DOI Creative Commons
Ilinca Suciu, Johannes Delp, Simon Gutbier

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 13(1), P. 49 - 49

Published: Dec. 28, 2023

To characterize the hits from a phenotypic neurotoxicity screen, we obtained transcriptomics data for valinomycin, diethylstilbestrol, colchicine, rotenone, 1-methyl-4-phenylpyridinium (MPP), carbaryl and berberine (Ber). For all compounds, concentration triggering neurite degeneration correlated with onset of gene expression changes. The mechanistically diverse toxicants caused similar patterns regulation: responses were dominated by cell de-differentiation canonical stress response pathways driven ATF4 NRF2. obtain more detailed specific information on modes-of-action, effects energy metabolism (respiration glycolysis) measured. Ber, rotenone MPP inhibited mitochondrial respiratory chain they shared complex I as target. This group was further evaluated metabolomics under experimental conditions that did not deplete ATP. Ber (204 changed metabolites) showed rotenone. overall metabolic situation characterized oxidative stress, an over-abundance NADH (>1000% increase) re-routing in order to dispose nitrogen resulting increased amino acid turnover. unique pattern led accumulation metabolites known biomarkers neurodegeneration (saccharopine, aminoadipate branched-chain ketoacids). These findings suggest inhibitors may result ensemble changes rather than simple ATP depletion. combi-omics approach used here provided richer MoA common analysis alone. As human drug food supplement, mimicked closely mode-of-action neurotoxicants, its potential hazard requires investigation.

Language: Английский

Citations

3
Synthesis and Biological Activity Research Progress of Phenanthridinone and Quinolinone Natural Products DOI

钰栋 马

Hans Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 12(02), P. 101 - 117

Published: Jan. 1, 2024

Language: Английский

Citations

0
Characterizing and identifying of miRNAs involved in berberine modulating glucose metabolism of Megalobrama amblycephala DOI
Mingyang Liu, Chang He, Tingting Zhu

et al.

Fish Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 50(4), P. 1667 - 1682

Published: July 4, 2024

Language: Английский

Citations

0
Mechanisms of Berberine in anti-pancreatic ductal adenocarcinoma revealed by integrated multi-omics profiling DOI Creative Commons
Jia Yang,

Tingting Xu,

Hongwei Wang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 2, 2024

Language: Английский

Citations

0
Analyses of Off‐Target Effects on Cardiac and Skeletal Muscles by Berberine, a Drug Used to Treat Cancers and Induce Weight Loss DOI
Jushuo Wang,

Yingli Fan,

Syamalima Dube

et al.

Cytoskeleton, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Previous reports from our laboratory describing the formation of myofibrils in cultured embryonic cardiac and skeletal muscle cells have proposed that myofibrillogenesis occurs three steps increasing protein organization: beginning with premyofibrils, followed by nascent myofibrils, ending mature myofibrils. Inhibitors ubiquitin proteasome system (UPS) prevented progressing directly to cells, supporting a three-step model assembly which some proteins are proteolyzed allow Application UPS inhibitors on suggests possible explanations for off-target adverse effects drugs, used cancer patients. Berberine, plant derivative, has been treat various cancers, including multiple myelomas. In contrast use success was reported Berberine myeloma patients no their hearts. We exposed ligase inhibitor UHRF1 (ubiquitin-like PHD RING finger domains). inhibited myofibril at stage but had effect heart cells. RT-PCR experiments demonstrated inhibition mRNA myosin II heavy chains not actin is also being as popular weight losing compound, because it much cheaper available without prescription than semaglutide containing drugs (Wegovy Ozempic). culture assays both postulate analyses will provide preclinical assay testing novel improved outcomes patients, an important goal therapeutics.

Language: Английский

Citations

0
Metabolomics and network pharmacology–guided analysis of TNF-α expression by Argemone mexicana (Linn) targeting NF-kB the signalling pathway in cancer cell lines DOI Creative Commons
Sunanda Kulshrestha, Anjana Goel, Subhadip Banerjee

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Dec. 2, 2024

Cancer has emerged as one of the leading causes fatality all over world. Phytoconstituents are being studied for their synergistic effects, which include disease prevention by altering molecular pathways and immunomodulation without side effects. The present experiment aims to explore cancer preventive activities

Language: Английский

Citations

0
Caryophylli Cortex Suppress PD-L1 Expression in Cancer Cells and Potentiates Anti-Tumor Immunity in a Humanized PD-1/PD-L1 Knock-In MC-38 Colon Cancer Mouse Model DOI Open Access
Aeyung Kim,

Eun‐Ji Lee,

Jung Ho Han

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(24), P. 4415 - 4415

Published: Dec. 23, 2024

Background/Objectives: Immune checkpoints are essential for regulating excessive autoimmune responses and maintaining immune homeostasis. However, in the tumor microenvironment, these can lead to cytotoxic T cell exhaustion, allowing cancer cells evade surveillance promote progression. The expression of programmed death-ligand 1 (PD-L1) is associated with poor prognoses, reduced survival rates, lower therapies. Consequently, downregulating PD-L1 has become a key strategy developing checkpoint inhibitors (ICIs). Caryophylli cortex (CC), derived from bark clove tree Syzygium aromaticum, possesses antioxidant properties against cells, yet its potential as an ICI remains unclear. Methods: In this study, we aimed investigate whether CC extract modulates activates immunity through co-culture system well hPD-L1/MC-38 tumor-bearing animal models. Results: Our findings indicate that significantly downregulated both constitutive inducible at non-toxic concentrations while simultaneously enhancing mortality activity system. Furthermore, administration mice resulted greater than 70% reduction growth increased infiltration CD8+ within microenvironment. Principal component analysis identified bergenin, chlorogenic acid, ellagic acid active ICIs. Conclusions: These suggest exerts potent antitumor effect blocker by inhibiting disrupting PD-1/PD-L1 interaction.

Language: Английский

Citations

0