The role of spinal neurons targeted by corticospinal neurons in central poststroke neuropathic pain DOI Creative Commons

Fenqqi Fan,

Tianze Yin,

Biwu Wu

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2024, Volume and Issue: 30(6)

Published: June 1, 2024

Abstract Background Central poststroke pain (CPSP) is one of the primary sequelae following stroke, yet its underlying mechanisms are poorly understood. Methods By lesioning lateral thalamic nuclei, we first established a CPSP model that exhibits mechanical and thermal hypersensitivity. Innocuous stimuli lesion evoked robust neural activation in somatosensory corticospinal neurons (CSNs), as well deep dorsal horn, where low threshold mechanosensory afferents terminate. In this study, used viral‐based mapping intersectional functional manipulations to decipher role CSNs their spinal targets pathophysiology. Results We mapped post‐synaptic lumbar innervating using an anterograde trans‐synaptic AAV1‐based strategy showed these interneurons were activated by innocuous tactile post‐thalamic lesion. Functionally, tetanus toxin‐based chronic inactivation targeted prevented development CPSP. Consistently, transient chemogenetic silencing alleviated hypersensitivity aversion linked contrast, was insufficient induce allodynia typically observed Conclusion The required but for establishment Our study provided novel insights into potential therapeutic interventions treat refractory central neuropathic conditions.

Language: Английский

The role of spinal neurons targeted by corticospinal neurons in central poststroke neuropathic pain DOI Creative Commons

Fenqqi Fan,

Tianze Yin,

Biwu Wu

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2024, Volume and Issue: 30(6)

Published: June 1, 2024

Abstract Background Central poststroke pain (CPSP) is one of the primary sequelae following stroke, yet its underlying mechanisms are poorly understood. Methods By lesioning lateral thalamic nuclei, we first established a CPSP model that exhibits mechanical and thermal hypersensitivity. Innocuous stimuli lesion evoked robust neural activation in somatosensory corticospinal neurons (CSNs), as well deep dorsal horn, where low threshold mechanosensory afferents terminate. In this study, used viral‐based mapping intersectional functional manipulations to decipher role CSNs their spinal targets pathophysiology. Results We mapped post‐synaptic lumbar innervating using an anterograde trans‐synaptic AAV1‐based strategy showed these interneurons were activated by innocuous tactile post‐thalamic lesion. Functionally, tetanus toxin‐based chronic inactivation targeted prevented development CPSP. Consistently, transient chemogenetic silencing alleviated hypersensitivity aversion linked contrast, was insufficient induce allodynia typically observed Conclusion The required but for establishment Our study provided novel insights into potential therapeutic interventions treat refractory central neuropathic conditions.

Language: Английский

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