What improvements do general exercise training and traditional Chinese exercises have on knee osteoarthritis? A narrative review based on biological mechanisms and clinical efficacy

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: May 22, 2024

Background Knee osteoarthritis (KOA) is a disease that significantly affects the quality of life patients, with complex pathophysiology includes degeneration cartilage and subchondral bone, synovitis, associations mechanical load, inflammation, metabolic factors, hormonal changes, aging. Objective This article aims to comprehensively review biological mechanisms clinical effects general exercise training traditional Chinese exercises (such as Tai Chi Qigong) on treatment KOA, providing references for development prescriptions. Methods A systematic search databases including PubMed, Web Science, Google Scholar, China National Knowledge Infrastructure (CNKI) was conducted, reviewing studies randomized controlled trials (RCTs), observational studies, reviews, meta-analyses. Keywords included “knee osteoarthritis,” “exercise therapy,” “physical activity,” “traditional exercise.” Results conclusion General positively KOA by such promoting blood circulation, improving metabolism inflammatory enhancing expression anti-inflammatory cytokines, reducing cell Traditional exercises, like Qigong, benefit improvement symptoms tissue repair regulating immune function alleviating joint inflammation. Clinical have shown both types can improve physical function, life, pain relief in patients KOA. Both are non-pharmacological options effectively patients’ physiological life. Future research should further explore long-term these interventions develop personalized programs based specific needs patients.

Language: Английский

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Piezo1 Activation Drives Enhanced Collagen Synthesis in Aged Animal Skin Induced by Poly L-Lactic Acid Fillers

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7232 - 7232

Published: June 30, 2024

Poly L-lactic acid (PLLA) fillers stimulate collagen synthesis by activating various immune cells and fibroblasts. Piezo1, an ion channel, responds to mechanical stimuli, including changes in extracellular matrix stiffness, mediating Ca

Language: Английский

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Construction of a 3D Degradable PLLA/β-TCP/CS Scaffold for Establishing an Induced Membrane Inspired by the Modified Single-Stage Masquelet Technique

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Although the Masquelet-induced membrane technique (MIMT) is now employed worldwide for bone defects, it often needs to be repeated and autogenous graft. This study aims investigate theoretical feasibility of replacing PMMA (poly(methyl methacrylate)) cement with PLLA (poly-l-lactic acid)/β -TCP (beta-tricalcium phosphate)/CS (calcium sulfate) scaffold single-stage defect reconstruction, which evoke induced (IM) formation in early stage directly acts as implantation second reconstruct defect. We constructed a corn-like PLLA/β -TCP/CS by fused deposition 3D printing method. The characterizations scaffolds were investigated systematically. P/T15/S15 (the 15% mass fraction β-TCP CS) filled into large-segmental radius defects white rabbits IMs. HE (hematoxylin–eosin) VG (van gieson) staining, along immunofluorescent performed analyze architecture cellularity, expression BMP-2 (bone morphogenetic protein-2), VEGF (vascular endothelial growth factor), TGF-β1 (transforming factor-β1) was evaluated IHC (immunohistochemistry) WB (western-blot) respectively, ALP (alkaline phosphatase) ARS (alizarin red S) staining applied assess osteogenic potential. PLLA/β-TCP/CS excellent physicochemical properties are successfully using technique. suggested that effectively mediated IM after 6 weeks placement. A significant presence M2 macrophages observed IM. results demonstrated IMs derived from exhibited elevated levels VEGF, BMP-2, TGF-β1, all promote differentiation BMSCs. cellular immunofluorescence, flow cytometry, indicate regulates phenotypic polarization M0 toward phenotype via PI3K/AKT/β-Catenin pathway. These findings suggest biodegradable may serve viable alternative owing its unique ability stimulate phenotype. work presents innovative materials strategies management defects.

Language: Английский

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IFT80 and TRPA1 cooperatively regulate bone formation by calcium signaling in response to mechanical stimuli

Metabolism, Journal Year: 2025, Volume and Issue: unknown, P. 156159 - 156159

Published: Feb. 1, 2025

Intraflagellar transport 80 (IFT80) is vital for primary cilia which can sense and transduce mechanical signals. Mechanical stimuli expedite osteoblastic differentiation bone formation in mesenchymal stem cells (MSCs). However, how IFT80 regulates transduction MSCs remains unclear. To investigate the role of development transduction, MSC-specific knock-out (Prx1Cre; IFT80f/f) mice were generated. These exhibited significant skeletal abnormalities. The study further examined effects deficiency on stimulation-induced formation, as well underlying molecular mechanisms involving TRPA1 calcium signaling pathways. In our study, Prx1Cre; IFT80f/f results pronounced abnormalities including dwarfism, defect, malformations skull, limbs, sternum, abnormal joint structures. Furthermore, inhibits stimulation induced differentiation. Exercise training could not improve mice. Mechanistically, downregulated expression transient receptor potential ankyrin 1 (TRPA1) TRPA1-mediated Ca2+ influx, inhibited under by AKT ERK Finally, overexpression reversed impaired exercise training. cooperatively regulate response to stimulation. findings suggest that are critical homeostasis may serve therapeutic targets disorders.

Language: Английский

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PGAM5 Modulates Macrophage Polarization, Aggravating Inflammation in COPD via the NF-κB Pathway

International Journal of COPD, Journal Year: 2025, Volume and Issue: Volume 20, P. 551 - 564

Published: March 1, 2025

Chronic obstructive pulmonary disease (COPD) has emerged as a very consequential issue threatening human life and health; therefore, research on its pathogenesis is urgently needed. A prior investigation discovered significant elevation in the phosphoglycerate mutase 5 (PGAM5) expression lung tissue of COPD smoking patients. This rise closely associated with severity. Nevertheless, precise molecular processes by which PGAM5 influences initiation advancement remain unknown. model was created using murine alveolar macrophages (MH-S). Flow cytometry, enzyme-linked immunosorbent assay, Western blotting, other methods were used to detect macrophage polarization, inflammatory factor secretion levels, changes nuclear factor-κB (NF-κB) pathway. stimulated M1 polarization proinflammatory factors interleukin-1β (IL-1β) tumor necrosis factor-alpha (TNF-α). bound activated apoptotic signaling-regulated kinase 1 (ASK1), further activating NF-κB These implications reversed when silenced. can cause an increase p-ASK1T838, trigger pathway activation, stimulate production factors. finding for preventing treating COPD.

Language: Английский

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CaMKK2 Regulates Macrophage Polarization Induced by Matrix Stiffness: Implications for Shaping the Immune Response in Stiffened Tissues

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Macrophages are essential for immune responses and maintaining tissue homeostasis, exhibiting a wide range of phenotypes depending on their microenvironment. The extracellular matrix (ECM) is vital component that provides structural support organization to tissues, with stiffness acting as key regulator macrophage behavior. Using physiologically relevant 3D stiffening hydrogel models, it found increased alone promoted polarization toward pro-regenerative phenotype, mimicking the effect interleukin-4(IL-4) in softer matrices. Blocking Calcium/calmodulin-dependent kinase 2 (CaMKK2) selectively inhibited stiffness-induced without affecting IL-4-driven pathways. In functional studies, CaMKK2 deletion prevented M2-like/pro-tumoral caused by stiffening, which turn hindered tumor growth. murine wound healing model, loss impaired stiffness-mediated accumulation, ultimately disrupting vascularization. These findings highlight critical role mechanosensitive fate determination gene expression program, positioning this promising therapeutic target modulate pathologically stiff tissues.

Language: Английский

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Mechanical forces in the tumor microenvironment: roles, pathways, and therapeutic approaches

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 12, 2025

Tumors often exhibit greater stiffness compared to normal tissues, primarily due increased deposition within the tumor stroma. Collagen, proteoglycans, laminin, and fibronectin are key components of extracellular matrix (ECM), interacting facilitate ECM assembly. Enhanced fiber density cross-linking result in elevated interstitial fluid pressure, subjecting tumors significant physical stress during growth. This mechanical is transduced intracellularly via integrins, Rho signaling pathway, Hippo thereby promoting invasion. Additionally, pressure fosters glycolysis cells, boosting energy production support metastasis. Mechanical cues also regulate macrophage polarization, maintaining an inflammatory microenvironment conducive survival. In summary, signals play a crucial role growth Understanding these their involvement progression essential for advancing our knowledge biology enhancing therapeutic approaches.

Language: Английский

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SIRT1 and exercise-induced bone metabolism: a regulatory nexus

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 26, 2025

Regular exercise positively influences bone health, enhances density and strength, reduces the risk of osteoporosis. Silent information regulator transcription 1 (SIRT1) is a deacetylase that plays pivotal role in regulation various biological processes. In this review, we explore SIRT1 modulating metabolism response to exercise. regulates crucial cellular processes, including inflammation, aging, autophagy, oxidative stress, cells such as marrow mesenchymal stem cells, osteoblasts, osteoclasts, exercise-induced stimuli. Notably, by muscle neurotransmitters, with acting key mediator. A comprehensive understanding SIRT1’s regulatory mechanisms will facilitate deeper exploration principles underlying improvements metabolism, ultimately providing novel insights into treatment metabolic disorders.

Language: Английский

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Biomimetic Piezoelectric Periosteum‐Bone Integrated Implant Promotes Bone Defect Repair by Remodeling Osteogenic Microenvironment

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Abstract Bone defects caused by trauma, infection, or tumors pose significant clinical challenges, particularly in large with poor healing outcomes. Traditional repair methods often fail to address the complex regenerative microenvironment. This study introduces a novel biomimetic piezoelectric periosteum‐bone bilayer implant designed remodel osteogenic microenvironment and enhance bone defect repair. The comprises an upper electrospun polyvinylidene fluoride‐curcumin‐loaded magnesium metal–organic framework (PVDF‐MOF/Cur, PMC) periosteum scaffold lower hydroxyapatite@gelatin methacrylate (HA@GelMA) (PMC+HA@GelMA, PMCG). In whole PMCG implant, PMC improves properties of PVDF enables sustained drug release via Mg‐MOF loaded Cur. Meanwhile, HA@GelMA facilitates marrow mesenchymal stem cells differentiation regeneration. Additionally, further accelerates promoting neuronal differentiation, as well enhancing angiogenesis regulating macrophage polarization. Transcriptome sequencing reveals that activates key signaling pathways associated angiogenesis, neurogenesis, inflammation regulation, osteogenesis, including HIF‐1α, PI3K‐Akt, JAK‐STAT, TGF‐β pathways. Thus, this work highlights multifunctional capabilities remodify accelerate repair, which offers promising platform for advancing tissue

Language: Английский

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Osteoimmunomodulatory biomaterials: Engineering strategies, current progress, and future perspectives for bone regeneration

Applied Materials Today, Journal Year: 2025, Volume and Issue: 44, P. 102753 - 102753

Published: April 29, 2025

Language: Английский

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