Fabrication and Temporal Dependency Osteogenic Regulation of Dual‐Scale Hierarchical Microstructures on Medical Metal Surface DOI
Hao Lü, Zhijun Li, Lida Zhu

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(31)

Published: Aug. 23, 2024

Abstract The structural characteristics at the interface of bone implants can guide biological regulation. In this study, a dual‐scale hierarchical microstructure is proposed and customized using hybrid machining to achieve temporal dependency osteogenic It observed that osteoblasts induced by structure exhibit adequate protrusion development rapid cell attachment through modulation mechanical forces in growth environment, further promot upregulation membrane receptor PDGFR‐α, which related proliferation. Afterward, transcriptomic analysis reveals during differentiation stage, DSH regulates cellular signaling cascades primarily integrin adhesion mechanisms then accelerates activating TGF‐β pathway cAMP pathway. Furthermore, calcium nodules are preferentially deposited within lower honeycomb‐like channels, thereby endowing with potential induce oriented deposition improve long‐term stability implant.

Language: Английский

Signalling interaction between β‐catenin and other signalling molecules during osteoarthritis development DOI Creative Commons
Jing Feng, Qing Zhang, Feifei Pu

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(6)

Published: Jan. 10, 2024

Abstract Osteoarthritis (OA) is the most prevalent disorder of synovial joint affecting multiple joints. In past decade, we have witnessed conceptual switch OA pathogenesis from a ‘wear and tear’ disease to entire joint. Extensive studies been conducted understand underlying mechanisms using genetic mouse models ex vivo tissues derived individuals with OA. These revealed that signalling pathways are involved in development, including canonical Wnt/β‐catenin its interaction other pathways, such as transforming growth factor β (TGF‐β), bone morphogenic protein (BMP), Indian Hedgehog (Ihh), nuclear κB (NF‐κB), fibroblast (FGF), Notch. The identification currently underway specific molecule(s) key pathway(s) playing decisive role development need be evaluated. This review will focus on recent progresses understanding critical β‐catenin TGF‐β, BMP, Notch, Ihh, NF‐κB, FGF. Understanding these novel insights into integration complex gene regulatory network during help us identify pathway leading discovery therapeutic strategies for intervention.

Language: Английский

Citations

7
The Role of Alarmins in the Pathogenesis of Rheumatoid Arthritis, Osteoarthritis, and Psoriasis DOI Creative Commons
Kajetan Kiełbowski, Wiktoria Stańska,

Estera Bakinowska

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(4), P. 3640 - 3675

Published: April 19, 2024

Alarmins are immune-activating factors released after cellular injury or death. By secreting alarmins, cells can interact with immune and induce a variety of inflammatory responses. The broad family alarmins involves several members, such as high-mobility group box 1, S100 proteins, interleukin-33, heat shock among others. Studies have found that the concentrations expression profiles altered in immune-mediated diseases. Furthermore, they involved pathogenesis conditions. aim this narrative review is to present current evidence on role rheumatoid arthritis, osteoarthritis, psoriasis. We discuss their potential involvement mechanisms underlying progression these diseases whether could become therapeutic targets. Moreover, we summarize impact pharmacological agents used treatment alarmins.

Language: Английский

Citations

6
Signaling pathway mechanisms of circadian clock gene Bmal1 regulating bone and cartilage metabolism: a review DOI Creative Commons

Yiting Ze,

WU Yong-yao,

Zhen Tan

et al.

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 27, 2025

Abstract Circadian rhythm is ubiquitous in nature. clock genes such as Bmal1 and Clock form a multi-level transcription-translation feedback network, regulate variety of physiological pathological processes, including bone cartilage metabolism. Deletion the core gene leads to alterations, while phenotypes are not consistent. Studies have shown that multiple signaling pathways involved process regulating metabolism, but exact regulatory mechanisms remain unclear. This paper reviews by which regulates bone/cartilage upstream factors control , current knockout mouse models for research. We hope provide new insights prevention treatment diseases related circadian rhythms.

Language: Английский

Citations

0
Exosomes in cartilage microenvironment regulation and cartilage repair DOI Creative Commons
Longfei Han,

Hou Wenyuan,

Weihua Fang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 5, 2025

Osteoarthritis (OA) is a debilitating disease that predominantly impacts the hip, hand, and knee joints. Its pathology defined by progressive degradation of articular cartilage, formation bone spurs, synovial inflammation, resulting in pain, joint function limitations, substantial societal familial burdens. Current treatment strategies primarily target pain alleviation, yet improved interventions addressing underlying are scarce. Recently, exosomes have emerged as subject growing interest OA therapy. Numerous studies investigated to offer promising therapeutic approaches for through diverse vivo vitro models, elucidating mechanisms which from various cell sources modulate cartilage microenvironment promote repair. Preclinical investigations demonstrated regulatory effects originating human cells, including mesenchymal stem cells (MSC), fibroblasts, chondrocytes, macrophages, derived Chinese herbal medicines, on modulation repair signaling pathways. Additionally, encompass matrix, proliferation migration autophagy, apoptosis, mitigation oxidative stress. An increasing number exosome carrier scaffolds under development. Our review adopts multidimensional approach enhance comprehension pivotal functions exerted sourced types OA. Ultimately, our aim pinpoint targets capable regulating facilitating

Language: Английский

Citations

0
Association between TGF-β1 and β-catenin expression in the vaginal wall of patients with pelvic organ prolapse DOI Creative Commons
Fei Xue, Dan Xu, Shurui Zhao

et al.

Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

Abstract The aim of this study is to investigate the mechanism action and correlation between transforming growth factor beta 1 (TGF-β1) β-catenin in pelvic organ prolapse (POP). compared vaginal wall tissues from two groups: 20 patients with POP (POP group) who had hysterectomies for benign conditions (control group). Hematoxylin Eosin Masson staining visualized collagen, while TUNEL detected apoptosis. Protein mRNA expression levels TGF-β1, β-catenin, matrix metallopeptidase 2 (MMP2), tissue inhibitor metalloproteinases (TIMP2), type I, alpha (COL1A1) were assessed using immunohistochemistry, quantitative real-time polymerase chain reaction, western blot techniques. Relationships protein expressions TGF-β1 COL1A1, COL1A1 analyzed. In group, collagen fibers sparse, disorganized, fragmented, fewer more apoptotic cells control group. TIMP2, significantly lower, MMP2 was higher ( p < 0.05). Positive correlations found COL1A1. Reduced may trigger by affecting floor metabolism.

Language: Английский

Citations

0
Exosomes Extracted from Human Umbilical Cord MSCs Contribute to Osteoarthritic Cartilage and Chondrocytes Repair Through Enhancing Autophagy While Suppressing the Wnt/β-Catenin Pathway DOI
Sharon Qin, Aijie Zhang, Lian Duan

et al.

Tissue Engineering and Regenerative Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Language: Английский

Citations

0
Primary cilia and inflammatory response: unveiling new mechanisms in osteoarthritis progression DOI Creative Commons

Yuyan Sun,

Zi-Yu Luo,

Yuanyuan Fu

et al.

Experimental Biology and Medicine, Journal Year: 2025, Volume and Issue: 250

Published: April 28, 2025

Osteoarthritis (OA) is a common degenerative joint disease that can lead to chronic pain and disability. The pathogenesis of OA involves low-grade inflammation, characterized by the degradation chondrocytes, inflammation synovium, systemic inflammation. This inflammatory response accelerates progression contributes functional impairment. Primary cilia play crucial role in cellular signal transduction maintenance cartilage matrix homeostasis, their dysfunction closely linked responses. Given these roles, primary may significantly contribute OA. review explores inflammation-associated signaling pathways OA, including NF-κB, MAPK, JAK/STAT, PI3K/AKT/mTOR signaling. In addition, we place particular emphasis on cilia-mediated modulation mediate chondrocyte responses mechanical loading cytokines via TRPV4, Hedgehog Notably, alterations length incidence chondrocytes during further underscore potential pathogenesis. identification biomarkers therapeutic targets related offers new for treatment management

Language: Английский

Citations

0
Fabrication and Temporal Dependency Osteogenic Regulation of Dual‐Scale Hierarchical Microstructures on Medical Metal Surface DOI
Hao Lü, Zhijun Li, Lida Zhu

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(31)

Published: Aug. 23, 2024

Abstract The structural characteristics at the interface of bone implants can guide biological regulation. In this study, a dual‐scale hierarchical microstructure is proposed and customized using hybrid machining to achieve temporal dependency osteogenic It observed that osteoblasts induced by structure exhibit adequate protrusion development rapid cell attachment through modulation mechanical forces in growth environment, further promot upregulation membrane receptor PDGFR‐α, which related proliferation. Afterward, transcriptomic analysis reveals during differentiation stage, DSH regulates cellular signaling cascades primarily integrin adhesion mechanisms then accelerates activating TGF‐β pathway cAMP pathway. Furthermore, calcium nodules are preferentially deposited within lower honeycomb‐like channels, thereby endowing with potential induce oriented deposition improve long‐term stability implant.

Language: Английский

Citations

0