Single‐cell sequencing: Current applications in various tuberculosis specimen types DOI Creative Commons
Yuqin Zeng, Quan Ma, Jinyun Chen

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(11)

Published: July 2, 2024

Abstract Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis ( M.tb ) and responsible for millions of deaths worldwide each year. It has complex pathogenesis that primarily affects the lungs but can also impact systemic organs. In recent years, single‐cell sequencing technology been utilized to characterize composition proportion immune cell subpopulations associated with TB since it high resolution surpasses conventional techniques. This paper reviews current use technologies in research their application analysing specimens from various sources TB, peripheral blood lung specimens. The focus on how these reveal dynamic changes subpopulations, genes proteins during progression after infection. Based findings, significant potential clinical value field research. Next, we will real‐world applications targets identified through diagnostics, therapeutics development effective vaccines.

Language: Английский

A Novel Rapid Detection Method for Mycobacterium tuberculosis Based on Scattering-Light Turbidity Using Loop-Mediated Isothermal Amplification DOI Creative Commons
Meimei Zeng, Xinru Wang,

Zifeng Tan

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 162 - 162

Published: March 3, 2025

The accurate detection of Mycobacterium tuberculosis (MTB) is a pressing challenge in the precise prevention and control tuberculosis. Currently, efficiency accuracy drug resistance for MTB are low, cross-contamination common, making it inadequate clinical needs. This study developed rapid nucleic acid method based on scattering loop-mediated isothermal amplification (LAMP). Specific primers MTB-specific gene (Ag85B) were designed, LAMP reaction system was optimized using self-developed turbidimeter. Experimental results showed that optimal included 1.5 µL 100 mmol/L magnesium ions, 3.5 10 dNTPs, 6 1.6 mol/L betaine, temperature 65 °C. minimum limit 12.40 ng/L, with fastest time being approximately min. exhibited good specificity, no bands other pathogens. Twenty culture-positive samples twenty culture-negative tested parallel; positive group 100%, (24.9 ± 13 min), there negative detection. features high efficiency, low cost, accuracy, effectively reduces cross-contamination, providing new technology MTB.

Language: Английский

Citations

0
Single‐cell sequencing: Current applications in various tuberculosis specimen types DOI Creative Commons
Yuqin Zeng, Quan Ma, Jinyun Chen

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(11)

Published: July 2, 2024

Abstract Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis ( M.tb ) and responsible for millions of deaths worldwide each year. It has complex pathogenesis that primarily affects the lungs but can also impact systemic organs. In recent years, single‐cell sequencing technology been utilized to characterize composition proportion immune cell subpopulations associated with TB since it high resolution surpasses conventional techniques. This paper reviews current use technologies in research their application analysing specimens from various sources TB, peripheral blood lung specimens. The focus on how these reveal dynamic changes subpopulations, genes proteins during progression after infection. Based findings, significant potential clinical value field research. Next, we will real‐world applications targets identified through diagnostics, therapeutics development effective vaccines.

Language: Английский

Citations

2