Premeiotic deletion of Eif2s2 causes oocyte arrest at the early diplotene stage and apoptosis in mice DOI Creative Commons
Wenjun Zhou, Biao Li,

Zhijuan Wang

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(12)

Published: July 24, 2024

Eukaryotic translation initiation factor 2 subunit (EIF2S2), a of the heterotrimeric G protein EIF2, is involved in translation. Our findings demonstrate that depletion Eif2s2 premeiotic germ cells causes oocyte arrest at pachytene and early diplotene stages 1 day postpartum (dpp) 5 dpp, respectively, eventually leads to apoptosis failure primordial follicle formation. Further studies reveal deletion downregulates homologous recombination-related mitochondrial fission-related levels, upregulates integrated stress response-related proteins mRNA levels. Consistently, significantly decreases expression dictyate genes compromises function, characterized by elongated shapes, decreased ATP levels mtDNA copy number, along with an excessive accumulation reactive oxygen species (ROS) superoxide. Furthermore, DNA damage response proapoptotic increase, while anti-apoptotic decrease Eif2s2-deleted mice. An increase oocytes positive cleaved-Caspase-3 TUNEL signals, alongside reduced Lamin B1 intensity, further indicates apoptosis. Collectively, meiotic stage impairing recombination, mainly through downregulation proteins, ROS subsequent damage.

Language: Английский

Premeiotic deletion of Eif2s2 causes oocyte arrest at the early diplotene stage and apoptosis in mice DOI Creative Commons
Wenjun Zhou, Biao Li,

Zhijuan Wang

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(12)

Published: July 24, 2024

Eukaryotic translation initiation factor 2 subunit (EIF2S2), a of the heterotrimeric G protein EIF2, is involved in translation. Our findings demonstrate that depletion Eif2s2 premeiotic germ cells causes oocyte arrest at pachytene and early diplotene stages 1 day postpartum (dpp) 5 dpp, respectively, eventually leads to apoptosis failure primordial follicle formation. Further studies reveal deletion downregulates homologous recombination-related mitochondrial fission-related levels, upregulates integrated stress response-related proteins mRNA levels. Consistently, significantly decreases expression dictyate genes compromises function, characterized by elongated shapes, decreased ATP levels mtDNA copy number, along with an excessive accumulation reactive oxygen species (ROS) superoxide. Furthermore, DNA damage response proapoptotic increase, while anti-apoptotic decrease Eif2s2-deleted mice. An increase oocytes positive cleaved-Caspase-3 TUNEL signals, alongside reduced Lamin B1 intensity, further indicates apoptosis. Collectively, meiotic stage impairing recombination, mainly through downregulation proteins, ROS subsequent damage.

Language: Английский

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