Altered placental immune cell composition and gene expression with isolated fetal spina bifida

American Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 91(3)

Published: March 1, 2024

Abstract Problem Fetal spina bifida (SB) is more common in pregnant people with folate deficiency or anomalies of metabolism. It also known that fetuses SB have a higher risk low birthweight, condition typically placental‐mediated. We therefore hypothesized fetal would associate altered expression key placental transporters and an increase Hofbauer cells (HBCs), which are folate‐dependent macrophages. Method study Folate receptor‐α (FRα), proton coupled receptor (PCFT), reduced carrier (RFC) protein localization (immunohistochemistry) HBC phenotypes (HBC abundance receptor‐ β [ FRβ ] expression; RNA situ hybridization) were assessed placentae from (cases; n = 12) term ( 10) gestational age (GA) – maternal body mass index matched controls without congenital anomalies. Results Cases had proportion villous HBCs (6.9% vs. 2.4%, p .0001) average (3.2 mRNA molecules per 2.3, .03) than GA‐matched controls. cases largely polarized to regulatory phenotype (median 92.1% HBCs). In sex‐stratified analyses, only male levels There no differences between groups the total percent syncytium stromal positive for FRα, PCFT, RFC immunolabeling. Conclusions by increased SB, suggesting immune‐mediated dysregulation phenotype, could contribute SB‐associated comorbidities.

Language: Английский

Altered placental phenotype and increased risk of placental pathology in fetal spina bifida: a matched case-control study

Placenta, Journal Year: 2024, Volume and Issue: 159, P. 107 - 118

Published: Dec. 8, 2024

Spina bifida (SB) remains one of the most common congenital anomalies and associates with significant comorbidities in fetus, which may, part, be driven by placental maldevelopment. We hypothesised that pathologies would more prevalent fetuses SB compared to without anomalies.

Language: Английский

Identification of novel nutrient sensitive human yolk sac functions required for embryogenesis

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 29, 2024

The human yolk sac (hYS) is essential for embryo nutrient biosynthesis/transport and development. However, there lacks a comprehensive study of hYS nutrient-gene interactions. Here we performed secondary analysis transcript profiles (n = 9 samples) to identify nutrient-sensitive genes regulatory networks, including those that associate with adverse perinatal phenotypes embryonic origins. Overall, 14.8% highly expressed are nutrient-sensitive; the most common cofactors metals B vitamins. Functional reveals more likely be involved in metabolic functions than not nutrient-sensitive. Through gene network analysis, find four transcription regulators (with zinc and/or magnesium cofactors) predicted collectively regulate 30.9% genes. Lastly, 117 an outcome Among these, greatest number linked congenital heart defects 54 genes), followed by microcephaly 37). Collectively, our characterises improves understanding ways which interactions may influence both typical pathological

Language: Английский