Assessment of Coastal Biodiversity: Eukaryotic Communuties Through DNA Metabarcoding Along Artificial Coastlines DOI
Aydın Kaleli, E. Gözde Özbayram, Reyhan Akçaalan

et al.

Published: Jan. 1, 2024

In natural coastal habitats, biofilms can support the growth of substrate-specific organisms. Organisms in flourish these areas and provide valuable ecological data for biomonitoring. this study, we aimed to assess eukaryotic community on substrates along coasts Istanbul. We used 18S rRNA gene-targeted metabarcoding reveal species diversity. The was composed a total 361 genera belonging 5 supergroups. Archaeplastida most abundant supergroup, it followed by Amorphea Arthropoda. PERMANOVA analysis revealed significant seasonal differences heterogeneous observed. Salinity, phosphorus, dissolved oxygen were main parameters causing variance community. Additionally, several non-indigenous species, such as Mytilus, high number observations temporal succession showed that is promising tool artificial hard substrates.

Language: Английский

Detecting Threatened Ichthyofauna in a Mediterranean Intensive Agricultural Landscape: From DNA Traces to Electrofishing DOI
Panagiota Xanthopoulou, Argyrios Sapounidis, Paraskevi Papadopoulou

et al.

Aquatic Conservation Marine and Freshwater Ecosystems, Journal Year: 2025, Volume and Issue: 35(4)

Published: April 1, 2025

ABSTRACT Intensive agriculture and farming activities, industry, as well human settlement can seriously affect freshwater ecosystems the species they host. These disturbances have been previously monitored using traditional catch surveys. New methods, such environmental DNA (eDNA) metabarcoding, significantly improve biodiversity mapping by accessing rarely visited areas enhancing detection of rare invasive species. We conducted an eDNA survey in a complex river tributary system at Tenagi, Philippi that hosts approximately 12.5% Greek ichthyofauna. A primer pair targeting 12S rRNA was used to analyse samples from 30 sites. reference sequence database generated regional diversity monitoring endemic, native fishes. Results were compared with data collected electrofishing stations allowed access. Two three invasive, two translocated 10 detected their current known distribution both methods. Overall, metabarcoding being more sensitive, consistently than methods on single fieldwork expedition. Co‐occurrence also investigated for first time, revealing minor variations composition within fish communities. Stations intensive agricultural zones potentially high accumulation organic inorganic pollutants (Tributary D), exhibited lower number reads richness other tributaries Tenagi. Our results demonstrate need complementary use assess assemblages habitats, combined approach address reliability issues biases associated each method.

Language: Английский

Citations

0
North Atlantic deep-sea benthic biodiversity unveiled through sponge natural sampler DNA DOI Creative Commons
Ramón Gallego, María Belén Arias, Andrea Corral‐Lou

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Aug. 19, 2024

The deep-sea remains the biggest challenge to biodiversity exploration, and anthropogenic disturbances extend well into this realm, calling for urgent management strategies. One of most diverse, productive, vulnerable ecosystems in deep sea are sponge grounds. Currently, environmental DNA (eDNA) metabarcoding is revolutionising field monitoring, yet complex benthic remain challenging assess even with these novel technologies. Here, we evaluate effectiveness whole-community characterise metazoan diversity grounds across North Atlantic by leveraging natural eDNA sampling properties sponges themselves. We sampled 97 tissues from four species North-Atlantic biogeographic regions screened them using universal COI barcode region. recovered unprecedented levels taxonomic per unit effort, especially phyla Chordata, Cnidaria, Echinodermata Porifera, at least 406 found our study area. These assemblages identify strong spatial patterns relation both latitude depth, detect emblematic currently employed as indicators habitats. remarkable performance approach different sponges, whole animal kingdom, illustrates vast potential samplers high-resolution biomonitoring solutions highly diverse ecosystems.

Language: Английский

Citations

3
A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species DOI Creative Commons
Jesús Zarcero, Adrià Antich, Marc Rius

et al.

iScience, Journal Year: 2023, Volume and Issue: 27(1), P. 108588 - 108588

Published: Nov. 25, 2023

Metabarcoding techniques are revolutionizing studies of marine biodiversity. They can be used for monitoring non-indigenous species (NIS) in ports and harbors. However, they often biased by inconsistent sampling methods incomplete reference databases. Logistic constraints prompt the development simple, easy-to-deploy samplers. We tested a new device called polyamide mesh organismal (POMPOM) with high surface-to-volume ratio. POMPOMS were deployed inside fishing recreational port Mediterranean alongside conventional settlement plates. also compiled curated database cytochrome oxidase (COI) sequences NIS. COI metabarcoding communities settled POMPOMs captured similar biodiversity than plates, shared molecular operational units (MOTUs) representing ca. 99% reads. 38 NIS detected accounting 26% easy to deploy handle provide an efficient method surveillance.

Language: Английский

Citations

5
Biodiversity patterns of the benthic macrofaunal communities across the intertidal sedimentary shores of two Antarctic islands DOI Creative Commons
Triana Revanales, Mariano Lastra,

A. Sánchez‐Mata

et al.

Marine Environmental Research, Journal Year: 2024, Volume and Issue: 194, P. 106333 - 106333

Published: Jan. 1, 2024

Antarctic benthic ecosystems support a unique fauna characterized by high levels of diversity and endemism. However, our knowledge the macrofauna communities across intertidal sedimentary shore is limited, their fundamental ecological features, including spatial variability, remain poorly understood. This study aimed to investigate abundance, community structure (i.e. taxa-specific abundance), biodiversity patterns (α-, β-, λ-diversity) on shores two islands (South Shetland archipelago, N Peninsula): Livingston Deception. We conducted quantitative sampling during Austral summer campaigns (2004 2005), studying eleven sites, with five sites located six Our results demonstrated significantly higher abundance than Furthermore, significant differences in were observed between islands. In terms patterns, there no number taxa within (α-diversity) variation composition (determined identity taxa) (β-diversity) observed, shedding light total count (λ-diversity) compared suggest that island-specific characteristics (e.g., granulometric characteristics, ice disturbance, sedimentation rates, geothermal activity) determine communities. other processes factors are operating different temporal scales population dynamics, biotic interactions, oceanographic conditions, climate change) influence occurrence macrofaunal taxa. findings contribute understanding variability these provide essential information for better management decisions conservation practices coastal ecosystems.

Language: Английский

Citations

1
Optimal biodiversity survey: Where to survey biodiversity in terrestrial Antarctica DOI Creative Commons

Xiang Zhao

Published: Jan. 1, 2024

To effectively conserve Antarctica's unique biodiversity and habitats, high-quality, comprehensive data is crucial. However, such are often incomplete biased. My thesis presents the first continent-wide assessment of spatial biases in region, revealing complex factors influencing survey locations beyond mere proximity to research stations. I also introduce a pioneering approach optimising future surveys amid limited resources, proposing designs that balance cost, coverage, existing biases. This work highlights importance strategic planning Antarctica offers model for improving collection biogeographical data.

Language: Английский

Citations

0
Assessment of Coastal Biodiversity: Eukaryotic Communuties Through DNA Metabarcoding Along Artificial Coastlines DOI
Aydın Kaleli, E. Gözde Özbayram, Reyhan Akçaalan

et al.

Published: Jan. 1, 2024

In natural coastal habitats, biofilms can support the growth of substrate-specific organisms. Organisms in flourish these areas and provide valuable ecological data for biomonitoring. this study, we aimed to assess eukaryotic community on substrates along coasts Istanbul. We used 18S rRNA gene-targeted metabarcoding reveal species diversity. The was composed a total 361 genera belonging 5 supergroups. Archaeplastida most abundant supergroup, it followed by Amorphea Arthropoda. PERMANOVA analysis revealed significant seasonal differences heterogeneous observed. Salinity, phosphorus, dissolved oxygen were main parameters causing variance community. Additionally, several non-indigenous species, such as Mytilus, high number observations temporal succession showed that is promising tool artificial hard substrates.

Language: Английский

Citations

0