Contrasting gut bacteriomes unveiled between wild Antheraea assamensis Helfer (Lepidoptera: Saturniidae) and domesticated Bombyx mori L. (Lepidoptera: Bombycidae) silkworms DOI
Gangavarapu Subrahmanyam,

Yeruva Thirupathaiah,

N. Vijay

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: May 22, 2024

Language: Английский

Establishment and application of a loop-mediated isothermal amplification method based on MetAP2 gene for the detection of Nosema bombycis in silkworms (Bombyx mori) DOI Creative Commons
Izhar Hyder Qazi, Ting Yuan,

Sijia Yang

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 10, 2025

Pebrine, caused by Nosema bombycis , is a devastating disease of silkworms that causes huge economic losses to the sericulture farmers. Although pebrine quarantine disease, currently development effective molecular diagnostic or therapeutic tools for its management still lagging hotspot in research. In present study, highly specific, sensitive, and field-applicable LAMP assay targeting MetAp2 gene was developed. LM1 primer set produced better results, with fluorescence (amplification) signals appearing ~50 min. The reaction temperature 60.9°C outer inner ratio 1:8 were found be optimal, shortest amplification time strongest intensity. showed high specificity DNA spores, as templates other common microorganisms no amplification. detected pMD-19T-met positive plasmid at lowest concentration 10 3 copies, detection ~80 practicality test can detect spore −3 ng/μL. At 1.2 ng/μL, real-time appeared ~60 all life stages untreated silkworms. fumagillin treated silkworms, observed 90 h later, indicating reliability detecting effectiveness fumagillin, some degree, treating infection. developed holds good promise application specific tool detection/control field settings.

Language: Английский

Citations

0
Biological and genomic characterization of a cypovirus isolated from golden muga silkworm, Antheraea assamensis Helfer (Lepidoptera: Saturniidae) DOI

Iyyangar Deepika,

Abhishek Singh,

Indira Kumar

et al.

Journal of Invertebrate Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 108343 - 108343

Published: April 1, 2025

Language: Английский

Citations

0
Development of Aptamer-Functionalized Gold Nanoparticles as Probes in Point-of-Care Diagnostic Device for Rapid Detection of Multidrug-Resistant Bacteria in Bombyx mori L. DOI
Rittick Mondal, Joydeep Chakraborty, Paulami Dam

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(8), P. 5740 - 5753

Published: Aug. 7, 2024

The sericulture industry suffers severe crop losses due to various silkworm diseases, necessitating the development of further technologies for rapid pathogen detection. Here, we report an all-in-one portable biosensor that combines conjugated gold nanoparticles (Au NPs) with aptamer-based lateral flow assay (LFA) platform real-time analysis

Language: Английский

Citations

3
Contrasting gut bacteriomes unveiled between wild Antheraea assamensis Helfer (Lepidoptera: Saturniidae) and domesticated Bombyx mori L. (Lepidoptera: Bombycidae) silkworms DOI
Gangavarapu Subrahmanyam,

Yeruva Thirupathaiah,

N. Vijay

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: May 22, 2024

Language: Английский

Citations

1