Investigating social communication in mice: a Two-intruders test approach DOI Creative Commons
Maryana V. Morozova, Lidiya V. Boldyreva,

Maria A. Borisova

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 27, 2023

Abstract Understanding the complex dynamics of social communication behaviors, such as exploration, communication, courtship, mating, and aggression in animal models is crucial to reveal key neural hormonal mechanisms underlying these behaviors. The Two-intruders test designed investigate residents’ behavior toward a male female intruders. During this imitating natural conditions, several aspects interaction were investigated: aggressive behavior. As mating involve overlapping circuits, behavioral setup testing both behaviors best at reflecting their competitive nature. Our findings demonstrate that mice exhibit strong preference communicate with intruder, which correlates baseline testosterone levels males. Relevant Two-intruder was also found BALB/c Behavioral breakdown revealed anogenital sniffing feature discriminates intruders different genders. At same time, accompanied by neuronal activation ventromedial hypothalamus. We odor recognition underlies females residents, experimental anosmia reduced intruder. However, there no correlation between contact smell test. assume be useful tool study neurological basis models. Combined tests, paradigm can help decipher circuits involved deficiency phenotypes human diseases. Significance Statement proves highly reproducible robust approach assess mice. results obtained setting replicate mouse groups strains. This indispensable studies assessing nature male- female-driven mechanisms. Resident’s interactions described here set up reflects processing circulating – physiological drivers conspecifics. While easy perform, provides broad spectrum patterns Largely overseen literature compared resident-intruder setup, provide superior performance when used understand communication.

Language: Английский

Investigating social communication in mice: a Two-intruders test approach DOI Creative Commons
Maryana V. Morozova, Lidiya V. Boldyreva,

Maria A. Borisova

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 27, 2023

Abstract Understanding the complex dynamics of social communication behaviors, such as exploration, communication, courtship, mating, and aggression in animal models is crucial to reveal key neural hormonal mechanisms underlying these behaviors. The Two-intruders test designed investigate residents’ behavior toward a male female intruders. During this imitating natural conditions, several aspects interaction were investigated: aggressive behavior. As mating involve overlapping circuits, behavioral setup testing both behaviors best at reflecting their competitive nature. Our findings demonstrate that mice exhibit strong preference communicate with intruder, which correlates baseline testosterone levels males. Relevant Two-intruder was also found BALB/c Behavioral breakdown revealed anogenital sniffing feature discriminates intruders different genders. At same time, accompanied by neuronal activation ventromedial hypothalamus. We odor recognition underlies females residents, experimental anosmia reduced intruder. However, there no correlation between contact smell test. assume be useful tool study neurological basis models. Combined tests, paradigm can help decipher circuits involved deficiency phenotypes human diseases. Significance Statement proves highly reproducible robust approach assess mice. results obtained setting replicate mouse groups strains. This indispensable studies assessing nature male- female-driven mechanisms. Resident’s interactions described here set up reflects processing circulating – physiological drivers conspecifics. While easy perform, provides broad spectrum patterns Largely overseen literature compared resident-intruder setup, provide superior performance when used understand communication.

Language: Английский

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