Wuzhuyu Decoction Relieves Chronic Migraine by Regulating 5-HT1A and 3A Receptors-Mediated CREB Signaling Pathway in Brain and Intestine DOI Creative Commons
Zhimin Song, Meijing Li, Ziwei Zhou

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 426 - 426

Published: March 18, 2025

Background: Chronic migraine (CM) is a common complex nervous system disease, often accompanied by symptoms of the digestive tract that interact with each other, leading to prolonged and difficult-to-cure migraines. These are associated abnormalities in 5-HT its receptors. Wuzhuyu decoction (WZYD) traditional Chinese medicine prescription commonly used clinics treat CM; it relieves gastrointestinal symptoms, such as nausea vomiting; however, mechanism still unclear. Investigating differences role WZYD compared existing drugs targeting receptors treatment CM not only helps elucidate pathogenesis but also provides possibilities for development new therapeutic approaches. Methods: An inflammation soup (IS)-induced male rat model was established. Based on preliminary experiment, target treating determined network pharmacology, verified molecular docking. ELISA, immunofluorescence, western blot, real-time quantitative polymerase chain reaction (RT-qPCR) were evaluate expression levels CM-related indicators (5-HT, calcitonin gene-related peptide (CGRP), c-Fos) ensure successful establishment effectiveness drug. On this basis, protein 5-HT1A/3A their cAMP-response element binding (CREB) signaling pathway detected blot immunohistochemistry. The validated using 5-HT1A receptor antagonist (WAY 100635) 5-HT3A agonist (SR 57227). Results: results showed increased brain, decreased CGRP, c-Fos, ionized calcium-binding adapter molecule 1 (Iba1), relieved CM. At same time, brain colon rats. Subsequently, further exerted brain-gut integrated effects regulating CREB mediated Conclusions: regulates neurotransmitters at specifically colon, which explains characteristics advantages from perspective. While effectively relieving headache improves related more conducive

Language: Английский

Wuzhuyu Decoction Relieves Chronic Migraine by Regulating 5-HT1A and 3A Receptors-Mediated CREB Signaling Pathway in Brain and Intestine DOI Creative Commons
Zhimin Song, Meijing Li, Ziwei Zhou

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 426 - 426

Published: March 18, 2025

Background: Chronic migraine (CM) is a common complex nervous system disease, often accompanied by symptoms of the digestive tract that interact with each other, leading to prolonged and difficult-to-cure migraines. These are associated abnormalities in 5-HT its receptors. Wuzhuyu decoction (WZYD) traditional Chinese medicine prescription commonly used clinics treat CM; it relieves gastrointestinal symptoms, such as nausea vomiting; however, mechanism still unclear. Investigating differences role WZYD compared existing drugs targeting receptors treatment CM not only helps elucidate pathogenesis but also provides possibilities for development new therapeutic approaches. Methods: An inflammation soup (IS)-induced male rat model was established. Based on preliminary experiment, target treating determined network pharmacology, verified molecular docking. ELISA, immunofluorescence, western blot, real-time quantitative polymerase chain reaction (RT-qPCR) were evaluate expression levels CM-related indicators (5-HT, calcitonin gene-related peptide (CGRP), c-Fos) ensure successful establishment effectiveness drug. On this basis, protein 5-HT1A/3A their cAMP-response element binding (CREB) signaling pathway detected blot immunohistochemistry. The validated using 5-HT1A receptor antagonist (WAY 100635) 5-HT3A agonist (SR 57227). Results: results showed increased brain, decreased CGRP, c-Fos, ionized calcium-binding adapter molecule 1 (Iba1), relieved CM. At same time, brain colon rats. Subsequently, further exerted brain-gut integrated effects regulating CREB mediated Conclusions: regulates neurotransmitters at specifically colon, which explains characteristics advantages from perspective. While effectively relieving headache improves related more conducive

Language: Английский

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