Methylene blue-encapsulated liposome for immobilization-free electrochemical immunoassay of interleukin-6 from nervous headache DOI
Nan Lin, Xiao Han, Shuo Tian

et al.

The Analyst, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Interleukin-6 (IL-6) protects neurons by inhibiting the expression of factors related to neuronal injury in nervous headache patients. The development rapid and sensitive IL-6 detection methods will be very advantageous for easing pain such In this work, an immobilization-free immunodetection method is explored voltammetric screening serum samples from Initially, methylene-blue-encapsulated liposomes (MBLS) labeled with anti-IL-6 antibodies are confined capture antibody-coated microplate through a sandwich-type immunoreaction, subsequently subjected lysis treatment. After that, lytic solution transferred into cell including Nafion-modified working electrode. Methylene blue molecules positive charge captured on negatively charged Nafion membrane, thus generating signal. peak currents relative amount solution. Under optimized experimental situations, MBLS-based split-type electrochemical sensing protocols have acceptable 0.01 100 pg mL-1, allow at concentration as low 9.1 fg mL-1. batch-to-batch coefficients variation were ≤11.95%. Good anti-interference capability was achieved against other biomolecules. Seven human specimens two diluted obtained patients determined immunoassay, well-matched results comparison those ELISA protocol.

Language: Английский

Methylene blue-encapsulated liposome for immobilization-free electrochemical immunoassay of interleukin-6 from nervous headache DOI
Nan Lin, Xiao Han, Shuo Tian

et al.

The Analyst, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Interleukin-6 (IL-6) protects neurons by inhibiting the expression of factors related to neuronal injury in nervous headache patients. The development rapid and sensitive IL-6 detection methods will be very advantageous for easing pain such In this work, an immobilization-free immunodetection method is explored voltammetric screening serum samples from Initially, methylene-blue-encapsulated liposomes (MBLS) labeled with anti-IL-6 antibodies are confined capture antibody-coated microplate through a sandwich-type immunoreaction, subsequently subjected lysis treatment. After that, lytic solution transferred into cell including Nafion-modified working electrode. Methylene blue molecules positive charge captured on negatively charged Nafion membrane, thus generating signal. peak currents relative amount solution. Under optimized experimental situations, MBLS-based split-type electrochemical sensing protocols have acceptable 0.01 100 pg mL-1, allow at concentration as low 9.1 fg mL-1. batch-to-batch coefficients variation were ≤11.95%. Good anti-interference capability was achieved against other biomolecules. Seven human specimens two diluted obtained patients determined immunoassay, well-matched results comparison those ELISA protocol.

Language: Английский

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