SNP discovery and genetic structure in blue mussel species using low coverage sequencing and a medium density 60 K SNP‐array

Evolutionary Applications, Journal Year: 2023, Volume and Issue: 16(5), P. 1044 - 1060

Published: April 25, 2023

Blue mussels from the genus Mytilus are an abundant component of benthic community, found in high latitude habitats. These foundation species relevant to aquaculture industry, with over 2 million tonnes produced globally each year. Mussels withstand a wide range environmental conditions and edulis complex readily hybridize regions where their distributions overlap. Significant effort has been made investigate consequences stress on mussel physiology, reproductive isolation, local adaptation. Yet our understanding genomic mechanisms underlying such processes remains limited. In this study, we developed multi medium-density 60 K SNP-array including four genus. SNPs included platform were called 138 23 distributed populations, sequenced using whole-genome low coverage approach. The array contains polymorphic which capture genetic diversity present populations thriving across gradient (~59 SNPs) set published validated informative for identification diagnosis transmissible cancer (610 SNPs). will allow consistent genotyping individuals, facilitating investigation ecological evolutionary these taxa. applications extend shellfish aquaculture, contributing optimization industry via selection blue mussels, parentage assignment, inbreeding assessment traceability. Further as genome association studies (GWAS) key production traits those related resilience especially safeguard under climate change.

Language: Английский

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Genomic selection for resistance to one pathogenic strain of Vibrio splendidus in blue mussel Mytilus edulis

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 3, 2025

The blue mussel is one of the major aquaculture species worldwide. In France, this faces a significant threat from infectious disease outbreaks in both farms and natural environment over past decade. Diseases caused by various pathogens, particularly Vibrio spp., have posed challenge to industry. Genetic improvement resistance can be an effective approach overcoming issue. work, we tested genomic selection (Mytilus edulis) understand genetic basis pathogenic strain splendidus (strain 14/053 2T1) predict accuracy using pedigree information. Additionally, performed genome-wide association study (GWAS) identify putative QTLs underlying resistance. We conducted experimental infection involving 2,280 mussels sampled 24 half-sib families containing each two full-sib which were injected with V. splendidus. Dead survivor all sampled, among them, 348 dead surviving genotyped recently published multi-species medium-density 60K SNP array. From potentially 23.5K SNPs for M. edulis present on array, identified 3,406 high-quality SNPs, out 2,204 successfully mapped onto reference genome. Heritability was moderate ranging 0.22 0.31 pedigree-based model 0.28 0.36 genomic-based model. GWAS revealed polygenic architecture trait mussel. models studied showed overall better performance than terms breeding values prediction. This work provides insights into exemplifies potential family-based programs edulis.

Language: Английский

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Decoding Local Adaptation in the Exploited Native Marine Mussel Mytilus chilensis: Genomic Evidence from a Reciprocal Transplant Experiment

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 931 - 931

Published: Jan. 23, 2025

Local adaptations are important in evolution as they drive population divergence and preserve standing genetic diversity essential for resilience under climate change human impacts. Protecting locally adapted populations is aquaculture species. However, high larval connectivity frequent translocations challenge this Chilean blue mussel (Mytilus chilensis) aquaculture, a world-class industry Chiloé Island. This study examined local two ecologically distinct natural beds, Cochamó (northernmost inner sea of Chiloé) Yaldad (southernmost tip), through 91-day reciprocal transplant experiment genomic evidence. mussels grew faster their native environment (0.015 g/day) than (0.004 g/day), though growth declined upon transplantation. Mussels transplanted within between beds displayed distinctive adaptive transcriptomic responses, with differentially expressed genes involved immune function, osmoregulation, metabolism, cellular balance. Additionally, 58 known outlier SNPs mapped over the species’ genome sequence were linked oxidative stress, oxygen management, revealing selection-targeted specific regions. highlights how affect response M. chilensis impact environments counterbalancing its connectivity, concluding that differences should be monitored conserved sustainable practices.

Language: Английский

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The mytilin gene cluster: shedding light on the enigmatic origin of mussel dispensable genes

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110286 - 110286

Published: March 1, 2025

Mussels exhibit a sophisticated innate immune response characterized by many highly variable molecules responsible for recognizing and killing potential pathogenic microorganisms. The complexity of this molecular arsenal is marked the occurrence gene presence-absence (PAV), phenomenon that targets numerous expanded lineage-specific families. This enhances inter-individual sequence variability, further enriching diversity repertoire involved in response. Until now, origin mussel dispensable genes, which, unlike core are not shared all individuals, has remained elusive. In study, analyzing resequenced genomes more than 160 individuals four distinct species Mytilus complex, we characterize mytilin genes encoding hemocyte-specific antimicrobial peptides (AMPs). We define canonical architecture comprising protein-coding two pseudogenes most haplotypes. However, organization locus displays intra-specific due to presence alleles, frequent pseudogenization G1 structural variants associated with additional which often retain features support functional preservation. Molecular phylogeny supports an ancient predating radiation modern species. suggests widespread extant haplotypes derive from larger complex ancestral cluster vestigial AMPs have been retained only few populations where their may contributed fitness advantages local adaptation.

Language: Английский

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Development and validation of a 66K SNP array for the hard clam (Mercenaria mercenaria)

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Sept. 9, 2024

Language: Английский

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Connectivity and population structure in a marginal sea – a review

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract The current biodiversity crisis calls for conservation measures that limit the negative human impact on important habitats and sensitive wild populations. To effectively protect at all levels, including intraspecific diversity, should be aligned with connectivity genetic structure of In this review, we synthesise scientific literature population marine species in Skagerrak – a marginal sea northeast Atlantic Ocean. We discuss results relation to management practices region, as well general transferability our findings. is one most intensively studied regions within research field, findings show overall adjacent seas high, but asymmetric, species. Simultaneously, have populations distinct from both each other, those seas. Most associated convoluted coastline common among coastal between offshore many mobile species, multiple are temporally sympatric certain areas, retain their divergence through natal homing or other barriers gene flow. presence despite high challenge area-based protection measures, flexible also monitors diversity timescales.

Language: Английский

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Patterns of admixture and introgression in a mosaic Mytilus galloprovincialis and Mytilus edulis hybrid zone in SW England

Molecular Ecology, Journal Year: 2023, Volume and Issue: 33(3)

Published: Dec. 8, 2023

Abstract The study of hybrid zones offers important insights into speciation. Earlier studies on populations the marine mussel species Mytilus edulis and galloprovincialis in SW England provided evidence admixture but were constrained by limited number molecular markers available. We use 57 ancestry‐informative SNPs, most which have been mapped genetically, to provide distinctive differences between admixed asymmetrical introgression from M. . combine genetic with analysis phenotypic traits potential ecological adaptive significance. demonstrate that individuals brown mantle edges unlike white or purple parental species, suggesting allelic non‐allelic genomic interactions. report gonad development stage consistent a prezygotic barrier species. By incorporating results publications dating back 1980, we confirm long‐term stability zone despite higher viability This coincides dramatic change temperature UK coastal waters suggests these might be resisting effects global warming. However, single SNP locus associated Notch transmembrane signalling protein shows markedly different pattern variation others adaptation colder northern temperatures.

Language: Английский

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SNPs Analysis Indicates Non-Uniform Origins of Invasive Mussels (Mytilus galloprovincialis Lamarck, 1819) on the Southern African Coast

Animals, Journal Year: 2024, Volume and Issue: 14(21), P. 3080 - 3080

Published: Oct. 25, 2024

Understanding the origins of invasive species is necessary to manage them and predict their potential for spreading. The mussel genus

Language: Английский

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Thermal selection shifts genetic diversity and performance in blue mussel juveniles

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Mussels from the genus Mytilus, key inhabitants of benthos, are important for aquaculture industry and one most sustainable sources animal protein available. Species within Mytilus edulis complex ( M. edulis, galloprovincialis trossulus ) commonly found in temperate regions globally can easily hybridise whenever their geographic distributions overlap. In Baltic Sea, populations formed by hybrids with low levels introgression. Given economic ecological relevance mussels, this study aimed to investigate mechanisms through which resilience towards global warming may be fast-tracked. For this, we developed two cohorts juvenile mussels (i.e. recently settled animals) Sea (Kiel, Germany), exposed an extreme heat event early life naïve stressor. Both were then experimental temperatures at proposed upper thermal limit population, 21 ° C 26 C, performance measured after 25 days. We assessed impacts stress on genetic composition each cohort genotyping 50 individuals using blue mussel 60K SNP-array. observed a significant increase genotypes together decrease S comparison NS juveniles. also that exposure high temperature has effect cohorts, reducing dry tissue weight selected individuals. Results provide relevant insights how selection juveniles, implications understanding managing under future scenarios.

Language: Английский

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SNP genotyping revealed a hybrid zone between Mytilus chilensis and M. platensis in southern South America (the Strait of Magellan, Isla Grande de Tierra del Fuego and the Falkland Islands)

Molecular Ecology, Journal Year: 2023, Volume and Issue: 33(2)

Published: Nov. 23, 2023

Abstract Hybrid zones among mussel species have been extensively studied in the northern hemisphere. In South America, it has only recently become possible to study natural hybrid zones, due clarification of taxonomy native mussels Mytilus genus. Analysing 54 SNP markers, we show genetic composition and admixture zone between M. chilensis platensis southern end America. Bayesian, non‐Bayesian clustering re‐assignment algorithms showed that Strait Magellan, Isla Grande de Tierra del Fuego Falkland Islands shows clinal architecture. The can be divided into three different areas: first one is on Atlantic coast where pure were found. second one, inside individuals both with variable degrees hybridisation coexist. last area at front Punta Arenas City, fjords Fuego, Beagle Channel, a low number hybrids According proportion hybrids, bays protected conditions away from strong currents would give better for hybridisation. We do not find evidence any other such as edulis, galloprovincialis, planulatus or trossulus zone.

Language: Английский

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