Genome Report: Chromosome-scale Genome Assembly of the West Indian fruit flyAnastrepha obliqua(Diptera: Tephritidae) DOI Open Access
Sheina B. Sim, Carlos Congrains, Sandra M. Velasco‐Cuervo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 26, 2023

Abstract The West Indian fruit fly, Anastrepha obliqua , is a major pest of mango in Central and South America attacks more than 60 species host fruits. To support current genetic genomic research on A. we sequenced the genome using high-fidelity (HiFi) long-read sequencing. This resulted highly contiguous contig assembly with 90% 10 contigs. was placed chromosomal context synteny closely related species, ludens as both are members fraterculus group. resulting represents five autosomes X chromosome which 95.9% genome, 199 unplaced contigs representing remaining 4.1%. Orthology analysis across structural annotation sets high quality tephritid genomes demonstrates gene annotations robust, identified genes unique to that may help define their pestiferous nature can be used starting point for comparative genomics. first this will serve foundation future its management an agricultural pest.

Language: Английский

Phylogenomic analysis provides diagnostic tools for the identification of Anastrepha fraterculus (Diptera: Tephritidae) species complex DOI Creative Commons
Carlos Congrains, Julian R. Dupuis, Erick J. Rodriguez

et al.

Evolutionary Applications, Journal Year: 2023, Volume and Issue: 16(9), P. 1598 - 1618

Published: Aug. 30, 2023

Insect pests cause tremendous impact to agriculture worldwide. Species identification is crucial for implementing appropriate measures of pest control but can be challenging in closely related species. True fruit flies the genus

Language: Английский

Citations

10
Insecticidal Potential of Baccharis macraei Essential Oils: An Ecological Approach to Their Volatile Composition in Insect Management DOI Creative Commons
Evelyn Muñoz-Núñez, Alejandro Madrid, Julio Alarcón

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 509 - 509

Published: Feb. 20, 2025

Bioinsecticides based on essential oils (EOs) are promising alternatives for agricultural insect control. In this study, the main compounds of Baccharis macraei EOs from two geographical areas were identified using gas chromatography–mass spectrometry (GC-MS). The insecticidal potential against Drosophila melanogaster, Tenebrio molitor, and Galleria mellonella was evaluated, along with acetylcholinesterase (AChE) inhibition molecular docking analysis. GC-MS analysis revealed oxygenated sesquiterpenes, such as spathulenol, caryophyllene oxide, α-cadinol. EO Site 1 (S1) exhibited activity lethal dose 50 (LD50) values 46.50, 465.76, 241.2 µg/mL D. T. G. mellonella, respectively. 2 (S2) showed LD50 74.81, 385.62, 195.2 same pests. AChE yielded inhibitory concentration (IC50) 12.8 ± 0.13 S1 11.7 0.07 S2. Molecular a strong binding energy between α-cadinol AChE. These results highlight that B. effective serve natural alternative to traditional insecticides.

Language: Английский

Citations

0
Intra- and interspecific temporal mating patterns in Anastrepha fraterculus and Anastrepha obliqua fruit flies DOI
Samira Chahad‐Ehlers, Jéssica Tagliatela, J. Oliveira

et al.

Chronobiology International, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 18

Published: March 3, 2025

Daily rhythms, such as mating times, play a key role in shaping insect behavior and are pivotal prezygotic reproductive isolation speciation. To investigate whether follows daily rhythm under natural light–dark cycles controlled temperature conditions, we examined the times of two related agricultural pest species, Anastrepha fraterculus obliqua. Our observations revealed distinct patterns their copulatory activities. A. shows unimodal pattern, peaking morning, while obliqua displays bimodal with occurring both morning late afternoon, all statistically validated. In obliqua, peak is more pronounced before winter solstice, reversing afterward. These results highlight adaptability these fruit flies' biological clocks, allowing them to adjust timing according seasonal environmental changes. findings also reveal how each species gauges durations, even if annual variation less tropical regions, twilight serving marker. The observed plasticity, including phase shifts amplitude changes emphasizes synchronization cycles, which may explain absence specific pre-mating behaviors initiation low-light seen fraterculus. This study underscores importance plasticity understanding fly behavior, implications for population management ecological dynamics, reinforces need 24-h capture rhythms fully.

Language: Английский

Citations

0
Genome report: chromosome-scale genome assembly of the West Indian fruit fly Anastrepha obliqua (Diptera: Tephritidae) DOI Creative Commons
Sheina B. Sim, Carlos Congrains, Sandra M. Velasco‐Cuervo

et al.

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: 14(4)

Published: Feb. 1, 2024

The West Indian fruit fly, Anastrepha obliqua, is a major pest of mango in Central and South America attacks more than 60 species host fruits. To support current genetic genomic research on A. we sequenced the genome using high-fidelity long-read sequencing. This resulted highly contiguous contig assembly with 90% 10 contigs. was placed chromosomal context synteny closely related species, ludens, as both are members fraterculus group. resulting represents five autosomes X chromosome which 95.9% genome, 199 unplaced contigs representing remaining 4.1%. Orthology analysis across structural annotation sets high quality tephritid genomes demonstrates gene annotations robust, identified genes unique to that may help define their pestiferous nature can be used starting point for comparative genomics. first this will serve foundation future its management an agricultural pest.

Language: Английский

Citations

3
Molecular evolution and genetic diversity of defective chorion 1 in Anastrepha fraterculus and Anastrepha obliqua (Diptera, Tephritidae) DOI Creative Commons

Mariana Cassia Bisio,

Edyane Moraes dos Santos,

Camilla A. Santos

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Abstract The family Tephritidae comprises numerous fruit fly species, some of which are economically significant, such as several in the genus Anastrepha. Most pest species this belong to fraterculus group, characterized by closely related that difficult differentiate due recent divergence and gene flow. Identifying genetic markers for their study is paramount understanding group’s evolution eventual phytosanitary control. Because there variation eggshell morphology among genus, we studied rapidly evolving defective chorion 1 (dec-1) gene, crucial formation reproduction. Fragments dec-1 from two most important Anastrepha fraterculus obliqua, were amplified compared transcriptome sequences, well sequences ludens, was used structure prediction. These data revealed three alternative transcripts A. consistent with patterns found other Tephritidae; obtained orthologous these tephritids NCBI investigate selection affecting at different hierarchical levels using methods. analyses show purifying has had an role across whole throughout its history, but evidence positive episodic diversifying levels. Different parts have shown distinct evolutionary rates, potentially correlating diverse proproteins produced posttranslational changes. This correlation appears evident certain lineages, including branch separates Anastrepha, internal branches within genus. Our showed shows remarkable exons, proven be informative changes hold promise not only studying differentiation Anastrepha also management selected species.

Language: Английский

Citations

0
Phylogenomic analysis and molecular identification of true fruit flies DOI Creative Commons

Rong He,

Shuping Wang, Qiang Li

et al.

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: June 21, 2024

The family Tephritidae in the order Diptera, known as true fruit flies, are agriculturally important insect pests. However, phylogenetic relationships of remain controversial. Moreover, rapid identification invasive flies is essential for plant quarantine but still challenging. To this end, we sequenced genome 16 fly species at coverage 47-228×. Together with previously reported genomes nine species, reconstructed trees using benchmarking universal single-copy ortholog (BUSCO), ultraconserved element (UCE) and anchored hybrid enrichment (AHE) gene sets, respectively. resulting 50% taxon-occupancy dataset each marker type were generally congruent 88% nodes both concatenation coalescent analyses. At subfamily level, Dacinae Trypetinae monophyletic.

Language: Английский

Citations

0
Molecular Markers for Analyses of Genetic Diversity within the Anastrepha fraterculus Complex with Emphasis on Argentine Populations DOI Creative Commons
Ludvik M. Gomulski, M. Teresa Vera, Silvia B. Lanzavecchia

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(10), P. 748 - 748

Published: Sept. 27, 2024

The South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to America where it is an extremely polyphagous pest of wild and cultivated fruits. It complex cryptic species currently composed eight recognised morphotypes: “Mexican”, “Venezuelan”, “Andean”, “Peruvian”, “Ecuadorian”, the three Brazilian morphotypes “Brazilian-1”, “Brazilian-2”, “Brazilian-3”. Molecular markers that can identify member are crucial for implementation effective control measures, such as sterile insect technique. object this study was evaluate use internal transcribed spacer 2 (ITS2) sequence discriminating several members A. (Mexican, Peruvian, Brazilian-1) related species, schultzi Blanchard. analysis highlighted significant genetic differentiation between evaluated morphotypes, allowed their discrimination within complex, provided new insights into relationships. ITS2 marker provides basis development taxonomic keys taxa complex. also represents important poorly studied schultzi.

Language: Английский

Citations

0
Genomics DOI

Seyyed Mohammad Amin Mousavi Sagharchi,

Mohsen Sheykhhasan, Atousa Ghorbani

et al.

Advances in bioinformatics and biomedical engineering book series, Journal Year: 2024, Volume and Issue: unknown, P. 23 - 68

Published: Sept. 27, 2024

Genomics is an exciting and rapidly evolving field at the intersection of biology, computer science, statistics, which has made remarkable strides in recent years thanks to advancements high-throughput sequencing technologies. This allows us analyze understand genetic code organisms, from humans microorganisms, shedding light on everything diagnosis treatment disorders identification disease-causing genes pathogens, mysteries biodiversity. With its wide range applications, genomics transforming our understanding life itself.

Language: Английский

Citations

0
Molecular evolution and genetic diversity of defective chorion 1 in Anastrepha fraterculus and Anastrepha obliqua (Diptera, Tephritidae) DOI
Mariana de Cássia Bisio,

Edyane Moraes dos Santos,

Camilla A. Santos

et al.

Development Genes and Evolution, Journal Year: 2024, Volume and Issue: 234(2), P. 153 - 171

Published: Nov. 7, 2024

Language: Английский

Citations

0
Genome Report: Chromosome-scale Genome Assembly of the West Indian fruit flyAnastrepha obliqua(Diptera: Tephritidae) DOI Open Access
Sheina B. Sim, Carlos Congrains, Sandra M. Velasco‐Cuervo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 26, 2023

Abstract The West Indian fruit fly, Anastrepha obliqua , is a major pest of mango in Central and South America attacks more than 60 species host fruits. To support current genetic genomic research on A. we sequenced the genome using high-fidelity (HiFi) long-read sequencing. This resulted highly contiguous contig assembly with 90% 10 contigs. was placed chromosomal context synteny closely related species, ludens as both are members fraterculus group. resulting represents five autosomes X chromosome which 95.9% genome, 199 unplaced contigs representing remaining 4.1%. Orthology analysis across structural annotation sets high quality tephritid genomes demonstrates gene annotations robust, identified genes unique to that may help define their pestiferous nature can be used starting point for comparative genomics. first this will serve foundation future its management an agricultural pest.

Language: Английский

Citations

0