Combine with RNA-seq Reveals the Effect of Melatonin in the Synthesis of Melanin in Primary Melanocytes of Silky Fowls Black-Bone Chicken DOI Open Access

Ting Yang,

Lingling Qiu, Shihao Chen

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(8), P. 1648 - 1648

Published: Aug. 18, 2023

(1) Background: It was found that the melanin of black-bone chicken has various effects such as scavenging DPPH free radicals and anti-oxidation, synthesis is affected by factors including hormones. In addition, several studies have melatonin affects melanoma cell melanin, which not been reported in primary melanocytes; so, relevant were conducted. (2) Methods: this study, melanocytes isolated characterized, then treated with different concentrations to investigate on terms synthesis-related genes, content, tyrosinase activity, combined RNA seq detect change gene expression level after treatment. (3) Results: We characterized melanocytes, indirect immunofluorescence assay results showed positive melanocyte marker genes. RT-qPCR decreased reduced content activity group. A total 1703 differentially expressed genes screened RNA-seq, KEGG results, signaling pathway associated synthesis, mTOR enriched. (4) Conclusions: Melatonin could decrease melanocytes.

Language: Английский

Melatonin and the Skin: Current Progress and Perspectives for Human Health DOI
Andrzej Słomiński, Tae Kim, Zorica Janjetović

et al.

Journal of Investigative Dermatology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

3
Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo DOI Open Access
Tara Samra, Tatiana Gomez Gomez, Kinga Linowiecka

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15963 - 15963

Published: Nov. 4, 2023

Human skin aging is associated with functional deterioration on multiple levels of physiology, necessitating the development effective senotherapeutics. The well-tolerated neurohormone melatonin unfolds anti-aging properties in vitro and vivo, but it remains unclear whether these effects translate to aged human ex vivo. We tested this organ-cultured, full-thickness eyelid (5-6 donors; 49-77 years) by adding culture medium, followed assessment core biomarkers via quantitative immunohistochemistry. Over 6 days, 200 µM significantly downregulated intraepidermal activity aging-promoting mTORC1 pathway (as visualized reduced S6 phosphorylation) MMP-1 protein expression epidermis compared vehicle-treated control skin. Conversely, transmembrane collagen 17A1, a key stem cell niche matrix molecule that declines aging, mitochondrial markers (e.g., TFAM, MTCO-1, VDAC/porin) were upregulated. Interestingly, 100 also increased epidermal VEGF-A protein, which required sufficient for inducing rejuvenation. In dermis, fibrillin-1 improved fibrillin structural organization, indicating an elastic fiber network. contrast, other (SIRT-1, lamin-B1, p16INK4, I) remained unchanged. This vivo study provides proof principle indeed exerts long-suspected never conclusively demonstrated surprisingly differential dermis.

Language: Английский

Citations

13
Balancing act: optimizing blue light for melanogenesis while minimizing cellular damage in primary human skin cells DOI Creative Commons
Augustin C. Barolet, Brice Magne, Karel Ferland

et al.

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 9, 2025

Recent findings show that visible light, particularly blue stimulates melanogenesis in human skin, though the underlying mechanisms remain debated. This study aimed to determine cell damage threshold of non-ionizing light on keratinocytes while preserving their ability stimulate melanogenesis. Human (N = 3) and melanocytes were isolated from skin samples varying Fitzpatrick phototypes irradiated with (λpeak 457 nm) UVA 385 nm). Cellular metabolic activity was assessed using AlamarBlue HS assay, α-Melanocyte-Stimulating Hormone (α-MSH) production by quantified ELISA, Western blotting used assess pro-melanogenic factor expression melanocytes. High intensity (50 mW/cm2, 50 J/cm2) (15 20 significantly reduced cellular activity, a 0.86 ± 0.055 0.60 0.031 (mean SD) fold decrease compared respective sham day 7. In contrast, moderate intensities (5-15 10-20 preserved stimulating α-MSH production, an optimal balance achieved at 10 15 J/cm2 (1.14 0.046 increase relative 7). Co-culture experiments confirmed enhanced via paracrine signaling, increasing Tyrosinase Dopachrome Tautomerase (DCT). Direct irradiation also increased pigmentation without significant damage. Moderate-intensity effectively maintaining both melanocytes, offering promising, safe approach for therapies targeting disorders.

Language: Английский

Citations

0
Human Hair Graying Revisited: Principles, Misconceptions, and Key Research Frontiers DOI Creative Commons
Ralf Paus, Alec Sevilla, James M. Grichnik

et al.

Journal of Investigative Dermatology, Journal Year: 2023, Volume and Issue: 144(3), P. 474 - 491

Published: Dec. 13, 2023

Language: Английский

Citations

8
Interleukin-15 is a hair follicle immune privilege guardian DOI
Takahiro Suzuki, Jérémy Chéret, FERNANDA DINELLI SCALA

et al.

Journal of Autoimmunity, Journal Year: 2024, Volume and Issue: 145, P. 103217 - 103217

Published: April 5, 2024

Language: Английский

Citations

3
Spatial Distribution and Functional Impact of Human Scalp Hair Follicle Microbiota DOI
Marta B. Lousada, J. Edelkamp, Tim Lachnit

et al.

Journal of Investigative Dermatology, Journal Year: 2023, Volume and Issue: 144(6), P. 1353 - 1367.e15

Published: Dec. 7, 2023

Language: Английский

Citations

8
Quercetin 3-O-(6″-O-E-caffeoyl)-β-D-glucopyranoside, a Flavonoid Compound, Promotes Melanogenesis through the Upregulation of MAPKs and Akt/GSK3β/β-Catenin Signaling Pathways DOI Open Access
Changhai Liu, Mayire Nueraihemaiti,

Deng Zang

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(5), P. 4780 - 4780

Published: March 1, 2023

Quercetin 3-O-(6″-O-E-caffeoyl)-β-D-glucopyranoside is a flavonoid compound produced by various plants with reported antiprotozoal potential against E. histolytica and G. lamblia; however, its effects on skin pigment regulation have not been studied in detail. In this investigation, we discovered that quercetin 3-O-(6″-O-E-caffeoyl)-D-glucopyranoside (coded as CC7) demonstrated more increased melanogenesis effect B16 cells. CC7 exhibited no cytotoxicity or effective stimulating melanin content intracellular tyrosinase activity. This melanogenic-promoting was accompanied activated expression levels of microphthalmia-associated transcription factor (MITF), key melanogenic regulatory factor, enzymes, (TYR) tyrosinase-related protein-1 (TRP-1) 2 (TRP-2) the CC7-treated Mechanistically, found exerted upregulating phosphorylation stress-regulated protein kinase (p38) c-Jun N-terminal (JNK). Moreover, upregulation phosphor-protein B (Akt) Glycogen synthase kinase-3 beta (GSK-3β) β-catenin cell cytoplasm, subsequently, it translocated into nucleus, resulting melanogenesis. Specific inhibitors P38, JNK, Akt validated promotes synthesis activity regulating GSK3β/β-catenin signaling pathways. Our results support involves MAPKs Akt/GSK3β/β-catenin

Language: Английский

Citations

5
Endocrine Controls of Skin Aging DOI Creative Commons
Markus Böhm, Agatha Stegemann, Ralf Paus

et al.

Endocrine Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Skin is the largest organ of human body and undergoes both intrinsic (chronological) extrinsic aging. While skin aging driven by genetic epigenetic factors, mediated external threats such as UV irradiation or fine particular matters, sum which referred to exposome. The clinical manifestations biochemical changes are different between aging, albeit overlapping features exist, eg, increased generation reactive oxygen species, extracellular matrix degradation, telomere shortening, lipid peroxidation, DNA damage. As a prominent target for many hormones, molecular processes underlying under tight control classical neuroendocrine axes. However, also an endocrine itself, including hair follicle, fully functional "miniorgan." Here we review pivotal hormones controlling focusing on IGF-1, key fibroblast-derived orchestrator GH, estrogens, retinoids, melatonin. emerging roles additional players, ie, α-melanocyte-stimulating hormone, central player hypothalamic-pituitary-adrenal axis; members hypothalamic-pituitary-thyroid oxytocin, endocannabinoids, peroxisome proliferator-activated receptor modulators, reviewed. Until now, only limited number these mainly topical retinoids have found their way into practice anti-skin compounds. Further research biological properties players its derivatives may offer development novel senotherapeutics treatment prevention

Language: Английский

Citations

1
Melatonin Protects K15+ Human Hair Follicle Stem Cells and Hair Matrix Keratinocytes against Paclitaxel-Induced Damage Ex Vivo DOI Creative Commons
Tara Samra, Jérémy Chéret, Jennifer Gherardini

et al.

Journal of Investigative Dermatology, Journal Year: 2023, Volume and Issue: 144(3), P. 697 - 701.e6

Published: Sept. 28, 2023

Language: Английский

Citations

3
Topically applied thyroid hormones stimulate hair growth in organ-cultured human scalp skin DOI Open Access
Jennifer Gherardini,

Aysun Akhundlu,

Matthew Gompels

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 14, 2024

Abstract We have previously shown that the thyroid hormones triiodothyronine (T3) and thyroxine (T4) prolong anagen, mitigate stem cell apoptosis, stimulate mitochondrial functions in microdissected human scalp HFs ex vivo. To circumvent systemic adverse effects of T3/T4, we asked current pilot study whether topically applied T3/T4 retains hair growth-promoting properties. prove this, treated healthy full-thickness skin with T3 (1, 10nM) T4 10μM) for six days serum-free organ culture, using an HF-targeting vehicle contains only FDA-approved ingredients. This showed that, at distinct doses, topical significantly increased percentage decreased proliferative (Ki-67+) cells matrix, did not promote melanogenesis (as measured by quantitative Warthin-Starry histochemistry), keratin 15 expression bulge. Finally, T4, low concentrations, increase growth promoters IGF-1 FGF-7. The lower concentration both also increases number CD31+ endothelial cells, suggesting a pro-angiogenic effect, which is important promotion. These preliminary results strongly suggest intact on multiple levels invites intermittent pulse application as novel therapeutic intervention managing loss disorders associated telogen effluvium, such androgenetic alopecia.

Language: Английский

Citations

0