Knockdown of OLFM4 protects cardiomyocytes from sepsis by inhibiting apoptosis and inflammatory responses DOI Open Access

Hailu Chen,

Hailu Chen,

Guihua Fang

et al.

Allergologia et Immunopathologia, Journal Year: 2024, Volume and Issue: 52(5), P. 15 - 20

Published: Sept. 1, 2024

Sepsis is a systemic inflammatory response that can result in cardiac insufficiency or heart failure known as septic myocardial injury. A previous study identified OLFM4 an important gene sepsis through bioinformatics analysis. However, there limited research on the regulatory functions of sepsis-triggered injury, and related molecular mechanisms remain unclear. In this study, protein expression was found to be significantly elevated LPS-stimulated H9C2 cells, its suppression enhanced cell proliferation reduced apoptosis LPS-triggered cells. The factors TNF-α, IL-6, IL-1β were increased after LPS treatment, these effects mitigated silencing OLFM4. Moreover, it confirmed inhibition attenuated NF-κB signaling pathway. conclusion, knockdown protected cardiomyocytes from by inhibiting responses via These findings provide insights into progression

Language: Английский

Histopathological effects of hypervitaminosis-D and the protective role of fetuin-A in renal, hepatic, and cardiac tissues in a murine model DOI Creative Commons
Maha Mohamed, Mohamed Hussein, Sarah Moustafa

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 7, 2025

Hypervitaminosis D leads to toxic effects, including hypercalcemia, which can cause severe damage various organs. Fetuin-A, a glycoprotein with anti-inflammatory properties, may protect tissues from such damage. This study explores the role of Fetuin-A in mitigating hypervitaminosis D-induced renal, hepatic, and cardiac tissues. The objectives this were to: (1) Assess extent tissue high-dose vitamin murine model by examining histopathological changes liver, kidney heart. (2) Investigate Fetuin-A's protective effect against Thirty-six albino rats divided into four groups: control, toxicity, (3) + D, (4) only. Vitamin was administered subcutaneously at 250 μg/20 g/day for 3 days. given 100 μl/20 g, starting 7 days before treatment. Histopathological analysis kidney, heart performed using H&E Alizarin Red staining findings analysed statistically. toxicity caused significant damage, apoptosis, inflammation, calcification kidneys, Pre-treatment reduced preserving architecture. Fetuin-A-only showed no or calcification. provided statistically protection reducing oxidative stress affected These suggest could be potential therapeutic agent D.

Language: Английский

Citations

2
JNK inhibition mitigates sepsis-associated encephalopathy via attenuation of neuroinflammation, oxidative stress and apoptosis DOI

Riya Gagnani,

Harshita Singh, Manisha Suri

et al.

Metabolic Brain Disease, Journal Year: 2025, Volume and Issue: 40(3)

Published: March 13, 2025

Language: Английский

Citations

0
JAK/STAT inhibitors mitigate sepsis‐associated cerebral and cognitive injury DOI

Mohini Singh Bhadauriya,

Harshita Singh, Manisha Suri

et al.

Fundamental and Clinical Pharmacology, Journal Year: 2025, Volume and Issue: 39(3)

Published: April 7, 2025

Abstract Background Sepsis is a life threatening condition which triggers multiple organ failure. Sepsis‐associated encephalopathy (SAE) more prevalent form of sepsis involves acute and long‐term cerebral impairment. JAK/STAT pathway one the most crucial signaling cascades promote neuroinflammation. Objectives The present investigation was designed to explore possible role inhibitors in sepsis‐induced injury cognitive impairment mice. Methods Swiss Albino mice underwent cecal ligation puncture (CLP) induce sepsis‐associated deficits. Tofacitinib baricitinib were administered orally hour before CLP, followed by six days post‐CLP administration. From 7‐12, behavioral changes assessed through various tests, including open field (locomotor activity non‐associative memory), inhibitory avoidance (aversive novel object recognition (recognition Morris‐Water maze tests (spatial learning memory). Neuronal (S‐100 calcium‐binding protein B, S100B neuronal specific enolase, NSE) inflammation (TNF‐α) serum. Further, oxidative mouse brain evaluated measuring malondialdehyde reduced glutathione levels. Results inhibitors, tofacitinib (7.5 15 mg/kgper os) (5 10 os), significantly ameliorated deficits non‐associative, aversive, spatial memory treatment decreased TNF‐α, Malondialdehyde, S‐100B NSE with while increasing levels glutathione. Conclusion neuroinflammation, stress, damage enhancing function.

Language: Английский

Citations

0
A focus on c-Jun-N-terminal kinase signaling in sepsis-associated multiple organ dysfunction: Mechanisms and therapeutic strategies DOI

Riya Gagnani,

Mugdha Srivastava, Manisha Suri

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 143, P. 113552 - 113552

Published: Nov. 15, 2024

Language: Английский

Citations

2
Polydopamine modification of polydimethylsiloxane for multifunctional biomaterials: Immobilization and stability of albumin and fetuin-A on modified surfaces DOI Open Access
Jie Li, Leah N. Barlow, Miguel Martinez Santos

et al.

Biointerphases, Journal Year: 2023, Volume and Issue: 18(6)

Published: Nov. 1, 2023

The surface of polydimethylsiloxane (PDMS) can be modified to immobilize proteins; however, most existing approaches are limited complex reactions and achieving multifunctional modifications is challenging. This work applies a simple technique modify PDMS using polydopamine (PDA) investigates immobilization multiple proteins. surfaces were characterized in detail stability was assessed, demonstrating that buffer solution, PDA modification maintained without an effect on properties. Bovine serum albumin (BSA) bovine fetuin-A (Fet-A) used as model biomolecules for simultaneous or sequential understand their use backfilling functionalization. Based 125I radiolabeling, amounts BSA Fet-A determined close double obtained control surfaces. Following elution with sodium dodecyl sulfate, around 67% 63% retained the surface. amount immobilized protein influenced by process (simultaneous sequential) affinity With modification, balanced level both proteins could achieved, whereas process, initially more strongly attached. After incubation plasma fetal serum, PDA-modified over 90% immobilized. demonstrates biological environments also play important role binding conjugated combination methods provides fundamental knowledge tailoring PDMS-based biomaterials applications cell-material interactions, biosensing, medical devices.

Language: Английский

Citations

1
Fetuin-A level in patients with untreated thyroid dysfunction DOI Creative Commons

Alaa Salman Idan,

MohdPouzi Hamzah,

Mahmoud Shakir Khudhair

et al.

The Ukrainian Biochemical Journal, Journal Year: 2024, Volume and Issue: 96(1), P. 60 - 72

Published: Feb. 23, 2024

Fetuin-A, a plasma glycoprotein, has been demonstrated to play an essential role in the pathogene­sis of several metabolic disorders. This study aimed estimate fetuin-A serum level patients with newly diagnosed primary hyperthyroidism (PHT) and subclinical hypothyroidism (SCH) examine its correlation thyroid hormones level, age sex patients. The involved 90 untreated dysfunction verified function test (45 PHT 45 SCH) control subjects. Triiodo­thyronin (T3), tetraiodothyronin (T4), stimulating hormone (TSH) concentrations were measured enzyme-linked fluorescent assay (ELFA), concentration was immunosorbent (ELISA). It that significantly higher group as compared showed significant positive T3 level. In SCH group, lower negative TSH Fetuin-A rose unaffected by all studied groups. perfect AUC value obtained for comparison between groups suggests potential use reliable diagnostic marker differentiate these two conditions.

Language: Английский

Citations

0
Total Flavonoids of Eucommia ulmoides Oliver Protects Cardiomyocytes against Lipopolysaccharide-Induced Injury by Regulating microRNA-494 Expression DOI Creative Commons

Chen Sun,

Xueli Ren,

Feini Wu

et al.

Indian Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 86(2)

Published: Jan. 1, 2024

To explore the protective mechanism of total flavonoids from Eucommia ulmoides Oliver leaves (thin-film electroluminescent) on lipopolysaccharide-induced cardiomyocyte H9C2 injury. Cardiomyocytes were divided into control, lipopolysaccharides, lipopolysaccharides+thin-film electroluminescent-low group, electroluminescent-middle, electroluminescent-high, lipopolysaccharides+microRNA-negative lipopolysaccharides+microRNA-494, electroluminescent+anti-microRNA-negative and electroluminescent+anti-microRNA-494 groups. The commercial kits used to evaluate superoxide dismutase glutathione peroxidase activities, as well malondialdehyde levels. Flow cytometry was utilized for estimation cell apoptosis. Western blotting employed detect cleaved caspase-3 caspase-9 protein Reverse transcription-quantitative polymerase chain reaction selected microRNA-494 expression. Lipopolysaccharides significantly decreased levels, increased induced apoptosis, upregulated levels in cardiomyocytes, suggesting that lipopolysaccharides facilitated oxidative stress Thin-film electroluminescent overtly weakened apoptosis a dose-dependent mode. partly overturned downregulation expression cardiomyocytes. Furthermore, elevation impaired lipopolysaccharides-induced In addition, knockdown thin-film mediated repressive effects could alleviate which achieved by upregulating

Language: Английский

Citations

0
Knockdown of OLFM4 protects cardiomyocytes from sepsis by inhibiting apoptosis and inflammatory responses DOI Open Access

Hailu Chen,

Hailu Chen,

Guihua Fang

et al.

Allergologia et Immunopathologia, Journal Year: 2024, Volume and Issue: 52(5), P. 15 - 20

Published: Sept. 1, 2024

Sepsis is a systemic inflammatory response that can result in cardiac insufficiency or heart failure known as septic myocardial injury. A previous study identified OLFM4 an important gene sepsis through bioinformatics analysis. However, there limited research on the regulatory functions of sepsis-triggered injury, and related molecular mechanisms remain unclear. In this study, protein expression was found to be significantly elevated LPS-stimulated H9C2 cells, its suppression enhanced cell proliferation reduced apoptosis LPS-triggered cells. The factors TNF-α, IL-6, IL-1β were increased after LPS treatment, these effects mitigated silencing OLFM4. Moreover, it confirmed inhibition attenuated NF-κB signaling pathway. conclusion, knockdown protected cardiomyocytes from by inhibiting responses via These findings provide insights into progression

Language: Английский

Citations

0