bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: April 21, 2023
Abstract
RNA
Binding
Proteins
(RBPs)
interact
with
and
ubiquitously
regulating
transcripts
during
their
life
cycle.
Previous
works
showed
that
RBPs
play
fundamental
roles
in
the
progression
of
angiogenesis-related
diseases.
However,
role
skeletal
endothelium-dependent
bone
formation
osteogenesis
is
unclear.
Here,
we
show
RBP-Ybx1
was
strongly
reduced
vasculature
from
ovariectomy-induced
osteoporotic
mice.
Endothelial
cell-specific
deletion
Ybx1
impaired
CD31
hi
EMCN
endothelium
morphology
resulted
low
mass,
while
its
overexpression
promoted
angiogenesis-dependent
ameliorated
loss
OVX
Mechanistically,
disrupted
CD31,
BMP4
stability
an
m5C-dependent
manner
blocked
endothelial-derived
release,
thereby
inhibiting
osteogenic
differentiation
BMSCs.
Administration
recombinant
protein
BMSCs
restored
iΔEC
Finally,
tail
vein
injection
CD31-modified
PEG-PLGA
carrying
sciadopitysin,
a
natural
agonist,
pharmacologically
partially
reversed
vessels
decline
improved
restoration
mass
both
aging
animals.
These
findings
demonstrated
provided
novel
therapeutic
approach
for
ameliorating
aging-related
postmenopausal
osteoporosis.
Life Science Alliance,
Journal Year:
2022,
Volume and Issue:
5(7), P. e202201403 - e202201403
Published: April 7, 2022
Dysfunction
of
RNA-binding
proteins
is
often
linked
to
a
wide
range
human
disease,
particularly
with
neurological
conditions.
Gemin5
member
the
survival
motor
neurons
(SMN)
complex,
ribosome-binding
protein
and
translation
reprogramming
factor.
Recently,
pathogenic
mutations
in
have
been
reported,
but
functional
consequences
these
variants
remain
elusive.
Here,
we
report
structural
deficiencies
associated
compound
heterozygosity
within
gene
found
patients
neurodevelopmental
disorders.
These
clinical
are
located
key
domains
Gemin5,
tetratricopeptide
repeat
(TPR)-like
dimerization
module
noncanonical
site
1
(RBS1).
We
show
that
TPR-like
disrupt
dimerization,
whereas
RBS1
variant
confers
instability.
All
mutants
defective
interaction
networks
involved
RNA-driven
pathways.
Importantly,
fail
associate
native
ribosomes,
hampering
its
involvement
control
establishing
difference
wild-type
protein.
Our
study
provides
insights
into
molecular
basis
disease
malfunction
Cell Biology International,
Journal Year:
2023,
Volume and Issue:
48(1), P. 20 - 30
Published: Nov. 17, 2023
Abstract
Short
for
pyruvate
kinase
M2
subtype,
PKM2
can
be
said
of
all‐round
player
that
is
notoriously
known
its
metabolic
involvement
in
glycolysis.
Holding
a
dural
role
as
or
non‐metabolic
(kinase)
enzyme,
has
drawn
extensive
attention
over
biological
roles
implicated
tumor
cells,
including
proliferation,
migration,
invasion,
metabolism,
and
so
on.
wandering
transboundary
both
intracellularly
extracellularly.
Specifically,
nuclear,
cytoplasmic,
mitochondrial,
exosomal,
even
circulate
within
the
body.
Importantly,
function
an
RNA‐binding
protein
(RBP)
to
self‐support
function.
Despite
investigations
reviews
available
surrounding
from
different
angles
little
been
described
regarding
some
novel
recently
found,
including,
example,
acting
protein,
protection
Golgi
apparatus,
remodeling
microenvironment,
forth.
Given
these
findings,
this
review,
we
summarize
recent
advancements
made
research,
mainly
respects.
By
way,
PKM1,
another
paralog
PKM2,
seems
have
overlooked
under‐investigated
since
discovery.
Some
discoveries
about
PKM1
are
also
preliminarily
mentioned
discussed.
Life,
Journal Year:
2024,
Volume and Issue:
14(5), P. 630 - 630
Published: May 15, 2024
This
review
systematically
investigates
the
critical
role
of
natural
binding
proteins
(NBPs),
encompassing
DNA-,
RNA-,
carbohydrate-,
fatty
acid-,
and
chitin-binding
proteins,
in
realms
oncology
diagnostics.
In
an
era
where
cancer
continues
to
pose
significant
challenges
healthcare
systems
worldwide,
innovative
exploration
NBPs
offers
a
promising
frontier
for
advancing
both
diagnostic
accuracy
therapeutic
efficacy
management
strategies.
manuscript
provides
in-depth
examination
unique
mechanisms
by
which
interact
with
specific
molecular
targets,
highlighting
their
potential
revolutionize
diagnostics
therapy.
Furthermore,
it
discusses
burgeoning
research
on
aptamers,
demonstrating
utility
as
'nucleic
acid
antibodies'
targeted
therapy
precision
Despite
applications
aptamers
enhancing
early
detection
developing
personalized
treatment
protocols,
this
identifies
knowledge
gap:
need
comprehensive
studies
understand
diverse
functionalities
potentials
across
different
types
scenarios.
By
bridging
gap,
underscores
importance
paving
way
next-generation
treatments.
International Journal of Molecular Medicine,
Journal Year:
2024,
Volume and Issue:
54(5)
Published: Sept. 16, 2024
Diabetic
foot
ulcer
(DFU)
is
a
destructive
complication
of
diabetes.
Negative
pressure
wound
therapy
(NPWT)
promotes
DFU
healing
through
an
undetermined
mechanism.
In
the
present
study,
RNA
sequencing
was
performed
on
granulation
tissue
from
3
patients
with
before
and
after
1
week
NPWT.
The
fused
in
sarcoma
(FUS)
interleukin
enhancer
binding
factor
2
(ILF2)
encoding
RNA‑binding
proteins
(RBPs)
were
screened
data,
samples
24
validated
analyzed
receiving
NPWT
by
reverse
transcription‑quantitative
PCR,
western
blotting
immunohistochemistry.
addition,
Frontiers in Oncology,
Journal Year:
2024,
Volume and Issue:
14
Published: Oct. 21, 2024
YB1
(Y
box
binding
protein
1),
a
multifunctional
capable
of
to
DNA/RNA,
is
present
in
most
cells
and
acts
as
splicing
factor.
It
involved
numerous
cellular
processes
such
transcription,
translation,
DNA
repair,
significantly
affecting
cell
proliferation,
differentiation,
apoptosis.
Abnormal
expression
this
closely
linked
the
formation
various
malignancies
(osteosarcoma,
nasopharyngeal
carcinoma,
breast
cancer,
etc.).
This
review
examines
multifaceted
functions
its
critical
role
osteosarcoma
progression,
providing
new
perspectives
for
potential
therapeutic
strategies.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 9, 2024
Abnormal
growth
of
blood
vessels
(choroidal
neovascularization)
can
lead
to
age-related
macular
degeneration
(AMD)
and
eventually
cause
vision
loss
due
detachment
the
retinal
pigmented
epithelium.
This
indicates
that
choroidal
neovascularization
is
important
for
treatment
AMD.
The
circadian
clock
in
mammalian
retina
responsible
controlling
various
functions
retina,
enabling
it
adjust
changes
light
darkness.
Recent
studies
have
revealed
a
potential
connection
between
eye
diseases,
although
cause-and-effect
relationship
has
not
been
definitively
established.
C57BL/6J
male
mice
(aged
6
weeks)
were
randomly
divided
into
two
groups
(Control
group:
9:00-21:00
period
(300
lx);
Jet
lag
8-hour
phase
advance
once
every
4
days).
A
laser-induced
CNV
model
was
created
after
2
weeks
feeding
controlled
or
jet-lagged
environment.
Then,
full
transcriptome
sequencing
performed.
pathways
regulated
by
differentially
expressed
mRNAs
identified
GO
analysis
GSEA.
Further
protein
networks
constructed
with
STRING
database
Cytoscape
software.
WGCNA
used
further
explore
co-expression
modules
these
differential
genes
correlation
phenotypes.
ceRNA
miRanda
TargetScan.
associated
overlapping
network
identified,
hub
validated
qPCR.
total
661
DEGs,
31
miRNAs,
106
lncRNAs
87
circRNAs
identified.
GSEA
showed
upregulated
DEGs
mainly
involved
reproductive
structure
development
system
development.
determine
interaction
relationships
DEGs.
expression
several
screened
each
module
separately.
Furthermore,
constructed.
target
DEmRNAs
function
wound
healing,
cell
spreading,
epiboly
epiboly,
morphogenesis
an
epithelial
sheet.
Finally,
ten
key
their
patterns
confirmed
real-time
In
this
study,
we
investigated
regulatory
mechanism
ceRNAs
according
different
light-dark
cycles
eyeball.
