Lactobacillus brevis GKJOY Supplementation Ameliorates Oxidative Stress and Reproductive Dysfunction in Male Rats with Polystyrene Microplastics-Induced Reproductive Toxicity DOI Open Access

Yi-Yuh Hwang,

Sabri Sudirman, Yu‐Chen Hsu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4533 - 4533

Published: May 9, 2025

The growing demand for plastic products has led to an increase in human exposure microplastics (MPs). MPs have been shown detrimental effects on reproductive function, while probiotics demonstrated promise enhancing fertility. This study aimed determine the protective of Lactobacillus brevis GKJOY against damage induced by polystyrene (PS-MPs) male rats. In cell study, LC540 cells were treated with L. postbiotic (PGK), gamma-aminobutyric acid (GABA), and PS-MPs evaluate their viability reactive oxygen species (ROS) production. animal experiment, rats a low dose (GK1X, 50 mg/kg), medium (GK2X, 100 or high (GK4X, 200 mg/kg). results showed that PGK GABA reduced levels ROS protected oxidative stress. contrast, increased had harmful viability. testicular injuries caused disruption hypothalamic–pituitary–gonadal (HPG) axis decrease hormone levels. However, treatment stress pro-inflammatory cytokine levels, restored hormonal imbalances, significant improvements. effectively mitigated due its dual function as probiotic neurotransmitter modulator. conclusion, GKJOY, which functions both producer, may offer superior protection damage.

Language: Английский

Clinical Electrophysiology and Mathematical Modeling for Precision Diagnosis of Infertility DOI Creative Commons

Fernanda Carvalho Cavalari,

Paola Sulis Mendes,

Bruna Antunes Zaniboni

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 250 - 250

Published: Jan. 21, 2025

How can cellular electrophysiology measurements and mathematical modeling of ionic channels help to identify pivotal targets in disease-related cell signaling? The purpose this review is highlight the advantages disadvantages using both these complementary techniques determine molecular that may be structurally or functionally altered a specific disease. In addition, improve coordinated drug development, accelerate prediction new drugs, facilitate repositioning pharmacological agents. This focuses on data obtained from model approaches, including intracellular recording, patch clamp measurements, Hodgkin Huxley equation, as key precision methodologies. To end, seminiferous tubules, Sertoli line (TM4), and/or primary cultures cells were used explore role follicle-stimulating hormone (FSH), thyroid hormones, retinol, testosterone, 1,25(OH)2 vitamin D3 activation inhibition essential for male fertility. Based discussed data, precisely regulate their biological activity by coordinating channel according hormonal environment nutritional requirements required germ development.

Language: Английский

Citations

0
Effects of semen inflammation on embryo implantation, placentation, pregnancy outcomes and offspring health DOI
María Santos Martínez,

Yair A. Chocobar,

Yamila Fariz

et al.

Placenta, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
The Biphasic Effect of Lipopolysaccharide on Membrane Potential DOI Creative Commons

Maria E. Hadjisavva,

Robin L. Cooper

Membranes, Journal Year: 2025, Volume and Issue: 15(3), P. 74 - 74

Published: March 2, 2025

Lipopolysaccharide (LPS) from certain strains of Gram-negative bacteria can induce a rapid (<1 s) hyperpolarization membrane potential, followed by gradual depolarization exceeding the initial resting potential. Through overexpression Drosophila ORK1 two-pore-domain K+ channel (K2P) in larval muscles and altering external concentrations Na+ ions, it is clear that due to activating K2P channels promoting an inward leak. When concentration negligible, LPS-delayed dampened. The induced LPS exceed −100 mV when are lowered. These results indicate direct action on ion independently immune responses. Such actions may need be considered developing clinical treatments for forms bacterial septicemia.

Language: Английский

Citations

0
Lactobacillus brevis GKJOY Supplementation Ameliorates Oxidative Stress and Reproductive Dysfunction in Male Rats with Polystyrene Microplastics-Induced Reproductive Toxicity DOI Open Access

Yi-Yuh Hwang,

Sabri Sudirman, Yu‐Chen Hsu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4533 - 4533

Published: May 9, 2025

The growing demand for plastic products has led to an increase in human exposure microplastics (MPs). MPs have been shown detrimental effects on reproductive function, while probiotics demonstrated promise enhancing fertility. This study aimed determine the protective of Lactobacillus brevis GKJOY against damage induced by polystyrene (PS-MPs) male rats. In cell study, LC540 cells were treated with L. postbiotic (PGK), gamma-aminobutyric acid (GABA), and PS-MPs evaluate their viability reactive oxygen species (ROS) production. animal experiment, rats a low dose (GK1X, 50 mg/kg), medium (GK2X, 100 or high (GK4X, 200 mg/kg). results showed that PGK GABA reduced levels ROS protected oxidative stress. contrast, increased had harmful viability. testicular injuries caused disruption hypothalamic–pituitary–gonadal (HPG) axis decrease hormone levels. However, treatment stress pro-inflammatory cytokine levels, restored hormonal imbalances, significant improvements. effectively mitigated due its dual function as probiotic neurotransmitter modulator. conclusion, GKJOY, which functions both producer, may offer superior protection damage.

Language: Английский

Citations

0