Assessing hybridization between non-native Procambarus clarkii (Girard, 1852) and native Procambarus troglodytes (Le Conte, 1855) (Decapoda: Astacidea: Cambaridae) in Atlantic drainages of the southeastern USA

Journal of Crustacean Biology, Journal Year: 2025, Volume and Issue: 45(1)

Published: Jan. 10, 2025

Abstract Crayfishes play important ecological and economic roles in the southeastern USA, with non-native crayfishes threatening many native species. While hybridization of crayfish species is observed both wild captive populations for some crayfishes, evidence genus Procambarus Ortmann, 1905 limited. The red swamp (Procambarus clarkii (Girard, 1852)) eastern troglodytes(Le Conte, 1855)) are two closely related to different basins USA. We used genetic markers assess potential these North Carolina South Carolina, A total 292 specimens were collected morphological identification distinguishing 134 P. 158 troglodytes individuals from syntopic (i.e., co-occurring) allopatric populations. Genetic microsatellite data was determine that no documented as hybrids our collections there indication recent or rampant between at any sampling locations. limited laboratory experiment showed successful mating egg fertilization but failed produce viable hybrid offspring. Although historical not study, events resulting inviable offspring may be having detrimental consequences warrant additional exploration.

Language: Английский

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Invasive Crayfish: Drivers or Passengers of Degradation in Freshwater Ecosystems?

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(2)

Published: March 1, 2025

ABSTRACT Invasive species, such as the freshwater crayfish Procambarus clarkii , reportedly negatively influence abundance of various aquatic species. Moreover, these invaders are increasingly linked to ecological degradation ecosystems, invaded habitats show increased levels turbidity, nitrogen, and organic matter concentration. P. has, among other impacts, been associated with eutrophication in habitats. However, observations suggest that presence is often not accompanied by ecosystem degradation, raising question whether they drivers or function passive passengers, being caused stressors. To investigate contrasting hypotheses, we conducted a full factorial experiment 24 mesocosms nutrient pollution (specifically N, P, K), ubiquitous stressor ecosystems. Here, assessed effects on community compositions morphologically identified macrophytes chironomids, well bacteria, phytoplankton, diatoms using environmental DNA (eDNA) metabarcoding. Nutrient induced significant shifts macrophyte biomass composition bacterial, diatom, phytoplankton communities. All microbial communities exposed initially diverged from control, after which bacterial converged back control final weeks. In contrast, found only marginal rendering it unlikely short‐ medium‐term driver tested biodiversity. As respond quickly changes conditions, results signify used study were relatively stable spite . The density timeframe studied may be leveraged threshold values design execution management strategies aim avert potential negative impacts structure. Ultimately, importance reinforced change

Language: Английский

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Imidacloprid Exposure Induced Impaired Intestinal Immune Function in Procambarus clarkii: Involvement of Oxidative Stress, Inflammatory Response, and Autophagy

Fishes, Journal Year: 2025, Volume and Issue: 10(3), P. 131 - 131

Published: March 17, 2025

Imidacloprid (IMI), a widely used neonicotinoid insecticide, has raised environmental concerns due to its potential impact on non-target aquatic organisms. This study investigates the effects of IMI exposure intestinal immune function red swamp crayfish (Procambarus clarkii, P. clarkii), focusing oxidative stress, inflammatory response, and autophagy. The clarkii was exposed different doses (0, 10.93, 21.86, 43.73, 87.45 μg/L) for 96 h. Our findings reveal that leads survival rate less than 70% when concentration μg/L at Hemolymph LZM AKP contents were significantly decreased medium high concentrations, expressions hsp70 nf-κb genes up-regulated. expression lysozyme gene down-regulated. Additionally, activities SOD, CAT, GPX decreased, MDA increased, CuZnsod, mMnsod, cat, gpx in gut down-regulated after medium-high IMI. autophagy-related showed beclin1, atg5, atg13, lc3c medium- high-concentration groups In summary, this elucidates levels impair through mechanisms involving

Language: Английский

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Population structure and mitogenomic analyses reveal dispersal routes of Macrobrachium nipponense in China

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: May 17, 2025

The oriental river prawn Macrobrachium nipponense is widely distributed in China, but its origin and distribution routes remain largely unknown. We collected 126 specimens from four lakes one across sequenced their mitochondrial cytochrome C oxidase subunit I (cox1) genes. performed whole-genome resequencing of 100 samples assembled mitogenomes for population analysis, these two types markers (cox1 all 13 protein-coding genes-13 PCGs), a nuclear marker (28S rRNA) SNPs to infer the relationships between five populations, structure, migratory routes. also complete mitogenome per sampled (5 total) used them conduct comparative mitogenomic analyses. comprised 15,774-15,784 base pairs (bp). average nucleotide diversity (π) inferred using cox1 gene data, was 0.03013 ± 0.00618, ranging 0.00500 0.00110 (Fuxian Lake) 0.03562 0.02538 (Khanka Lake). identified haplotypes (33 101 PCGs) clustered into three main geographical lineages. Lineage A included Khanka Lake clade Haihe River. Fuxian constituted lineage B. majority River, Taihu Lake, Poyang minority Lake. This study indicates that native M. prawns China originated East subsequently spreading northward westward inland regions along Grand Canal Yangtze River system, forming distinct proposed route improves our understanding geographic China.

Language: Английский

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No evidence that crayfish carcasses produce detectable environmental DNA (eDNA) in a stream enclosure experiment

PeerJ, Journal Year: 2020, Volume and Issue: 8, P. e9333 - e9333

Published: June 11, 2020

Environmental DNA (eDNA) is an emerging tool for monitoring invasive and imperiled species, particularly at low densities. However, the factors that control eDNA production, transport, persistence in aquatic systems remain poorly understood. For example, extent to which carcasses produce detectable unknown. If positive detections are associated with dead organisms, this could confound or species. Here, we present results from one of first studies examine carcass situ by deploying red swamp crayfish (Procambarus clarkii) a stream enclosure experiment 28 days. We predicted would initially decline over time as decayed. Unsurprisingly, lost biomass time, but conclusion our much carapace chelae remained. no P. clarkii was detected any samples density (15 ∼615 g initially), flow (520-20,319 L/s), temperature (∼14-25 °C) site. Subsequent analyses demonstrated these were not consequence PCR inhibition field samples, poor performance assay intraspecific genetic diversity within clarkii, due preservation extraction procedure used. Therefore, suggest when relatively rare, such cases new populations endangered may eDNA. In scenarios, be more confidently attributed presence live organisms. recommend future should explore how biomass, flow, differences system (lentic vs. lotic) influence ability detect carcasses.

Language: Английский

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