Evolution and adaptation of dengue virus in response to high temperature passaging in mosquito cells

Virus Evolution, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 1, 2025

Abstract The incidence of arboviral diseases like dengue, chikungunya, and yellow fever continues to rise in association with the expanding geographic ranges their vectors, Aedes aegypti albopictus. distribution these vectors is believed be driven part by climate change increasing urbanization. Arboviruses navigate a wide range temperatures as they transition from ectothermic (from 15°C 35°C) humans (37°C) back again, but role that temperature plays driving evolution arboviruses remains largely unknown. Here, we passaged replicate dengue serotype-2 virus populations 10 times at either 26°C (Low) or 37°C (High) C6/36 albopictus cells explore differences adaptation thermal environments. We then deep-sequenced resulting tested replicative fitness an all-cross regime. also assessed ability viruses insect vector. While both regimes accumulated substitutions, only those reared treatments exhibited nonsynonymous changes, including several E, envelope protein, multiple non-structural genes. Passaging higher led reduced mosquitoes. One mutations E gene involved loss glycosylation site previously shown reduce infectivity These findings suggest selected for growth ambient may experience tradeoffs between thermostability replication Such associations might have implications suitability transmission under changing climate.

Language: Английский

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First evidence of a causal link between genetic variation and thermal adaptation in a schistosome host snail

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 2, 2024

Abstract Freshwater snails are pivotal in transmitting schistosomiasis, a tropical parasitic disease affecting over 150 million people. The adaptive potential of these is critical factor determining how climate change and other environmental factors influence transmission dynamics, yet it has remained unexplored. Bulinus truncatus the schistosome intermediate host snail with widest geographic distribution therefore plays role maximum range urogenital schistosomiasis. In this study, we assessed local adaptation capacity B. to temperature through an integrative approach encompassing phenotypic, ecophysiological, genomic data. Ten populations from diverse thermal environments were collected three countries, eight reared common garden. F2 generation (total N= 2592) was exposed chronic treatments various life-history traits recorded for 14 weeks. Subsequently, ecophysiological analyses conducted on ten last surviving per population. Genotyping parental field using genotyping-by-sequencing (GBS) approach, revealed 12,875 single nucleotide polymorphisms (SNPs), which 4.91 % potentially under selection. We observed significant association between outlier SNPs, temperature, precipitation. Thermal adaptations evident, lower survival rates at high temperatures warm- origin compensated by higher reproduction rates. Cold-origin snails, hand, exhibited growth adapted shorter growing season. Ecophysiological included elevated sugar haemoglobin contents cold-adapted snails. contrast, warm-adapted displayed increased protein levels but also more oxidative damage. Furthermore, heightened phenoloxidase indicated robust immune response parasite-rich regions. substantial holds profound implications its change, future schistosomiasis risk, effectiveness control measures. Highlights Local influences species’ responses showed (LA) LA apparent variations life history identified genetic basis underlying hosts could sustain global warming

Language: Английский

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Infectious disease responses to human climate change adaptations

Global Change Biology, Journal Year: 2024, Volume and Issue: 30(8)

Published: Aug. 1, 2024

Abstract Many recent studies have examined the impact of predicted changes in temperature and precipitation patterns on infectious diseases under different greenhouse gas emissions scenarios. But these scenarios symbolize more than altered regimes; they also represent differing levels change energy, transportation, food production at a global scale to reduce effects climate change. The ways humans respond change, either through adaptation or mitigation, underappreciated, yet hugely impactful disease transmission, often complex sometimes nonintuitive ways. Thus, addition investigating direct diseases, it is critical consider how human preventative measures adaptations will alter environments hosts that support pathogens. Here, we responses likely risk both positive negative We evaluate evidence for impacts based available data, identify research directions needed address while minimizing externalities associated with disease, especially vulnerable communities. several are affect independently itself. categorize into strategies secure access water, food, shelter, mitigation decrease emissions. recognize consequences under‐resourced communities, call attention need socio‐ecological connect behavioral their disease. Understanding crucial as intensifies community builds momentum slow health, economic productivity, political stability.

Language: Английский

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Morphological changes in eggs and embryos of Aedes aegypti (Diptera: Culicidae) exposed to predicted climatic scenarios for the year 2100 in the Central Amazon

Acta Tropica, Journal Year: 2024, Volume and Issue: 258, P. 107328 - 107328

Published: July 18, 2024

Language: Английский

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Morphometry of Aedes Aegypti Eggs Exposed to the Climatic Conditions Predicted by the Ipcc for the End of the 21st Century

Published: Jan. 1, 2024

According to the IPCC, by year 2100, rises in global temperature could reach up 5 oC above current averages. On a planet-wide scale, this is one of effects climate changes that have repercussions on biological cycle Aedes aegypti, main arbovirus vector urban environments and transmitter arboviruses cause dengue, Zika, chikungunya yellow fever. The objective study was evaluate morphological Ae. aegypti eggs embryos maintained change simulator. For this, specimens obtained from an insectarium were kept four chambers simulated range environmental scenarios predicted IPCC for 2100. each room collected transported laboratory morphometric analysis, using confocal scanning microscopy. (n=20) used obtain following variables: total width, length, length-width ratio diameter micropylar disc. Additionally, 20 data head capsule width ratio. subjected normality test means variable compared ANOVA Tukey's post-hoc test, considering (p < 0.05). A significant reduction 0.05) mean egg length observed when samples under climatic conditions. greater current, mild intermediate extreme scenario, which they significantly reduced results suggest morphometry as result influences adults subjected, may impact population density and, consequently, dynamics environments.

Language: Английский

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Taking stock of selective logging in the Andaman Islands, India: recent & legacy effects of timber extraction, assisted natural regeneration and a revamped working plan

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 27, 2024

ABSTRACT Forest management is an evolving balance between biodiversity conservation and economic needs. A shift in Andaman Islands’ Working Plan mandate 2000s reflects this evolution. Our study independently assesses the impact of said policy change on post-logging recovery forests Baratang Middle Andaman. In 2017-18, we placed seventy-six 0.49ha plots across evergreen deciduous patches compared large-tree (≥180cm girth) density diversity that were logged after 2005 focussing sustainability, 1990s timber, twice 2005, unlogged forests. We assessed forest regeneration thirty 0.01ha along a coupe road within 2005. Forests had similar large trees as (despite having 1/3 rd time), indicating reduced offtake or better recruitment. Along unlogged—once-logged—twice-logged gradient, dominance Pterocarpus dalbergioides decreased while Diptercarpus sp. increased. Compared to natural regeneration, proportionately more saplings planted both patches. The new working plan maintains timber stock but not diversity. make six simple recommendations align practice with mandate. Synthesis Post-2005 extraction Islands partially successful long-term health, line mandate, requires (1) lower from (2) targeted assisted non-timber tree species.

Language: Английский

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Physiological Basis of Parasitism

CABI eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 18

Published: July 12, 2024

Language: Английский

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Genomics-driven monitoring of Fraxinus latifolia (Oregon Ash) for conservation and EAB-resistance breeding

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Abstract Understanding the evolutionary processes underlying range-wide genomic variation is critical to designing effective conservation and restoration strategies. Evaluating influence of connectivity, demographic change, environmental adaptation for threatened species can be invaluable proactive potential. In this study, we assessed across range Fraxinus latifolia , a foundational riparian tree native western North America recently exposed invasive emerald ash borer ( Agrilus planipennis ; EAB). Over 1,000 individuals from 61 populations were sequenced using reduced representation (ddRAD-seq) species’ range. Strong population structure was evident along latitudinal gradient, with connectivity largely maintained central valley river systems, center diversity coinciding major systems Despite evidence estimates nucleotide size low all populations, suggesting patchy distribution F. may impact its long-term Range-wide offset, which indicate change required adjust future climate projections, greatest in eastern lowest southern portions range, regional potential longer-term. To preserve capacity needed development climate-resilient, EAB-resistant breeding programs, prioritizing will provide foundation management long-term.

Language: Английский

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Evolutionary adaptation under climate change:Aedessp. demonstrates potential to adapt to warming

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 24, 2024

Abstract Climate warming is expected to shift the distributions of mosquitoes and mosquito-borne diseases, facilitating expansions at cool range edges contractions warm edges. However, whether mosquito populations could maintain their through evolutionary adaptation remains unknown. Here, we investigate potential for thermal in Aedes sierrensis , a congener major disease vector species that experiences large gradients its native range, by assaying tolerance prolonged acute heat exposure, genetic basis diverse, field-derived population. We found pervasive evidence heritable variation tolerance, which phenotypically trades off with exposure. A simple model based on our data shows estimated maximum rate typically exceeds projected climate under idealized conditions. Our findings indicate natural may have track via adaptation. Prior climate-based projections thus underestimate future Significance Statement Global change profound impacts distribution collectively cause nearly one million deaths each year. Accurately predicting these critical control preparedness, will depend, part, can adapt warming—a key open question. Using experimental genomic from relative already wide gradient, find high levels genetically-based enable pace warming. Incorporating adaptive responses therefore be necessary accurate predictions warming, preparing interventions.

Language: Английский

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Prolonged thermal stress enhances mosquito tolerance to viral infection

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Summary How and to what extent mosquito-virus interaction is influenced by climate change a complex question of ecological epidemiological relevance. We worked at the intersection between thermal biology vector immunology studied shifts in tolerance resistance cell fusing agent virus (CFAV), prominent component mosquito virome know contribute shaping competence, warm-acclimated warm-evolved Aedes albopictus mosquitoes. show that length challenge influences outcome infection with mosquitoes being more tolerant CFAV infection, while resistant suffering from extensive fitness costs. These results highlight importance considering fluctuations immunity relation understand natural variation response viruses frame realistic transmission models.

Language: Английский

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