Calcium-sensing receptors promoted Homer1 expression and osteogenic differentiation in bone marrow mesenchymal stem cells DOI Creative Commons

Kainan Liu,

Tianjie Xu,

Jiaxin Fan

et al.

Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

Homer1 interacts with calcium-sensing receptors (CaSRs) in osteoblasts (OBs), both CaSR and playing essential roles AKT phosphorylation. This study investigated the impact of on expression during differentiation rat bone marrow mesenchymal stem cells (BMSCs) at morphological, imaging, molecular levels, vivo vitro. A post-oophorectomy osteoporosis model was established Sprague-Dawley rats, validated through micro-computed tomography, hematoxylin-eosin staining, biomechanical testing to assess changes expression. BMSCs were isolated from 3 week-old SD rats for vitro OB studies, wherein osteogenic induced alongside Morphological alterations examined using transmission electron microscopy immunofluorescence staining. Furthermore, protein mRNA levels OB-specific genes quantified by Western blot reverse transcription quantitative real-time polymerase chain reaction, Homer1-related proteins also assessed. Results showed a reduction ovariectomized group. In cellular activation upregulated AKT, Homer1, markers, promoting cell differentiation. conclusion, enhances differentiation, likely via Homer1-mediated regulation signaling, suggesting as potential therapeutic target osteoporosis.

Language: Английский

Calcium-sensing receptors promoted Homer1 expression and osteogenic differentiation in bone marrow mesenchymal stem cells DOI Creative Commons

Kainan Liu,

Tianjie Xu,

Jiaxin Fan

et al.

Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

Homer1 interacts with calcium-sensing receptors (CaSRs) in osteoblasts (OBs), both CaSR and playing essential roles AKT phosphorylation. This study investigated the impact of on expression during differentiation rat bone marrow mesenchymal stem cells (BMSCs) at morphological, imaging, molecular levels, vivo vitro. A post-oophorectomy osteoporosis model was established Sprague-Dawley rats, validated through micro-computed tomography, hematoxylin-eosin staining, biomechanical testing to assess changes expression. BMSCs were isolated from 3 week-old SD rats for vitro OB studies, wherein osteogenic induced alongside Morphological alterations examined using transmission electron microscopy immunofluorescence staining. Furthermore, protein mRNA levels OB-specific genes quantified by Western blot reverse transcription quantitative real-time polymerase chain reaction, Homer1-related proteins also assessed. Results showed a reduction ovariectomized group. In cellular activation upregulated AKT, Homer1, markers, promoting cell differentiation. conclusion, enhances differentiation, likely via Homer1-mediated regulation signaling, suggesting as potential therapeutic target osteoporosis.

Language: Английский

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