Inhibitory effect of tannic acid on the growth of Apiospora arundinis and 3-Nitropropionic acid production DOI
Wenyan Jiang, Xuelian Liang,

Huiling Li

et al.

Journal of Applied Microbiology, Journal Year: 2023, Volume and Issue: 134(12)

Published: Nov. 13, 2023

This study aimed to investigate the inhibitory effect of tannic acid (TA) on growth Apiospora arundinis and 3-Nitropropionic (3-NPA) production.To antifungal mechanism, effects TA hypha growth, electrical conductivity, morphology, defense-related enzymes, 3-NPA production A. were studied. concentrations 640 1280 μg ml-1 exhibited strong activity against arundinis. The results scanning electron microscopy transmission showed that was severely deformed after treatment, cell membrane blurred thin, vacuoles obviously shrunken smaller, most organelles decomposed into irregular fragments. increased conductivity malondialdehyde content indicated caused peroxidation unsaturated fatty acids damaged structure membrane. decrease intracellular ATPase succinate dehydrogenase function mitochondria, participated in inhibition respiratory metabolism. In addition, significantly reduced completely inhibited at ml-1.TA effectively both vitro production.

Language: Английский

Efficacy and Antifungal Mechanism of Rosemary Essential Oil against Colletotrichum gloeosporioides DOI Open Access
Tiantian Yuan, Hua Yang, Dangquan Zhang

et al.

Forests, Journal Year: 2024, Volume and Issue: 15(2), P. 377 - 377

Published: Feb. 18, 2024

The antifungal activity and mechanism of rosemary essential oil against Colletotrichum gloeosporioides, the walnut anthracnose pathogen, were investigated using scanning electron microscopy (SEM), index determination transcriptome technique. results showed that could inhibit growth C. gloeosporioides with minimum inhibitory (MIC) fungicidal (MFC) concentrations 15.625 μL/mL 31.25 μL/mL, respectively. Scanning revealed mycelium morphology became shriveled, twisted, severely deformed after being treated oil. chitinase, which decomposes fungal cell wall components in increased. ergosterol content plasma membrane decreased, while contents including nucleic acids, soluble protein reducing sugar released resulting extracellular electrical conductivity changed. For metabolic activity, enzymes succinate dehydrogenase (SDH), malate (MDH), ATPase ATP whereas phosphofructokinase (PFK) Transcriptome sequencing involves destruction membrane, inhibition genetic material synthesis, division differentiation. are helpful to understand efficacy provide a theoretical basis for development as biological control agent.

Language: Английский

Citations

11
Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani DOI Creative Commons
Chongqing Zhang, Wei Chen, Bin Wang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 15, 2024

Fusarium solani is a pathogenic fungus that causes significant harm, leading to crop yield reduction, fruit quality postharvest decay, and other diseases. This study used potato glycoside alkaloids (PGA) as inhibitors investigate their effects on the mitochondrial structure tricarboxylic acid (TCA) cycle pathway of F. . The results showed PGA could inhibit colony growth (54.49%), resulting in disappearance membrane loss contents. significantly decreased activities aconitase (ACO), isocitrate dehydrogenase (IDH), α-ketoglutarate (α-KGDH), succinate (SDH), fumarase (FH), malate (MDH), succinyl-CoA synthetase (SCS), increased activity citrate synthase (CS) After treatment, contents acetyl coenzyme A (CoA), citric (CA), malic (L-MA), α-ketoglutaric (α-KG) were decreased. isocitric (ICA), succinyl (S-CoA), succinic (SA), fumaric (FA), oxaloacetic (OA) increased. Transcriptomic analysis affect expression levels 19 genes related TCA RT-qPCR ACO, IDH, α-KGDH, MDH-related down-regulated, SDH FH-related up-regulated, which was consistent with transcriptomics. In summary, can achieve antifungal by reducing cycle’s flow regulating key genes’ levels. reveals mechanism from perspective cycle, provides theoretical basis for development application biopesticide.

Language: Английский

Citations

6
Effect of Potato Glycoside Alkaloids on Energy Metabolism of Fusarium solani DOI Creative Commons
Chongqing Zhang,

Dedong Ding,

Bin Wang

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(7), P. 777 - 777

Published: July 24, 2023

Fusarium solani is one of the primary pathogens causing root rot wolfberry. The aims this study were to investigate inhibitory effect potato glycoside alkaloids (PGA) on F. and its energy metabolism. In study, effects PGA treatment growth development investigated changes in glycolytic pathway (EMP), ATPase activity, mitochondrial complex structure, charge level analyzed elucidate possible antifungal mechanism solani. results showed that inhibited colony growth, biomass, spore germination reduced glucose content Hexokinase (HK) activity solani, but increased Fructose-6-Phosphate Kinase (PFK) Pyruvate (PK) promoted accumulation pyruvic acid. addition, activities H+-ATPase, Ca2+-ATPase, IV, inner membrane Ca2+ permeability transition pore, decreased contents ATP, ADP, AMP as well charge. These indicate inhibits activates glycolysis pathway, destroys structure function membrane, resulting a lower level.

Language: Английский

Citations

10
Graphene oxide-based nanocomposites for outstanding eco-friendly antifungal potential against tomato phytopathogens DOI
Abeer S. Elsherbiny,

Alyaa Galal,

Khalid M. Ghoneem

et al.

Biomaterials Advances, Journal Year: 2024, Volume and Issue: 160, P. 213863 - 213863

Published: April 18, 2024

Language: Английский

Citations

4
Effects of cinnamon essential oil on the physiological metabolism of Salmonella enteritidis DOI Creative Commons
Zhen Zhang, Yuanyuan Zhao, Xueqin Chen

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 6, 2022

Food safety and health are the themes of today's society. As a class foodborne pathogens, Salmonella enteritidis has become one common zoonotic pathogens. Because chemical preservatives have certain harmfulness been questioned, it is particularly important to find green safe natural preservatives. The advantages plant essential oils (EOs) that they safe, wide range antibacterials, not easy form drug resistance. In recent years, studies found EOs excellent antibacterial activity, but their mechanism conclusive, which limitations in application food field. Cinnamon oil (CEO) extracted from dried cinnamon secondary metabolite cells very flavor. More importantly, non-toxic human body proven good effect, its still unclear. Therefore, was great practical significance carry out research on CEO S. enteritidis. this work, used as test bacteria, selected agent study mechanisms. By studying physiological metabolism by CEO, influence bacteriostatic systematically elucidated. treatment would reduce activity bacterial metabolism. It mainly reflected following three aspects: first, key enzymes TCA circulation inhibited, thus affecting respiration Second, affects level energy inhibiting content adenosine triphosphate (ATP) ATPase. Finally, can affect bacteria proteins other substances. article expected provide theoretical basis for development new prevention control

Language: Английский

Citations

15
Targeted amino acid consumption and respiratory depression to study the antibacterial mechanism of linalool against Shigella sonnei DOI
Rongrong He, Weijun Chen, Qiuping Zhong

et al.

Food Control, Journal Year: 2023, Volume and Issue: 155, P. 110058 - 110058

Published: Aug. 18, 2023

Language: Английский

Citations

9
Enhancement of agri-food by-products: green extractions of bioactive molecules with fungicidal action against mycotoxigenic fungi and their mycotoxins DOI Creative Commons
Paola Giorni,

Giulia Bulla,

Giulia Leni

et al.

Frontiers in Nutrition, Journal Year: 2023, Volume and Issue: 10

Published: May 25, 2023

Today, alternative strategies based on the use of bioactive compounds have been proposed to reduce mycotoxin contamination and limit chemical fungicides.In present work, several by-products collected from agri-food chain (i.e., red white grape marc, grapevine leaves, seeds stalks, pear, apple, green beans, tomato, spent hops) were subjected extraction protocols steam distillation, Ultrasound-Assisted, Naviglio® extraction) obtain extracts rich in polyphenols terpenes. Each extract was assessed vitro for its ability inhibit development main mycotoxigenic species related mycotoxins.Aspergillus flavus A. carbonarius significantly reduced by pear (from -45 -47%) marc -21 -51%) extracts, while F. graminearum shown be highly influenced stalk, (-24% average). On contrary, verticillioides inhibited only (-18%) a very low negligible extent apple (-1%) beans (-3%). Regarding reduction mycotoxins, able OTA 2 57%, AFB1 5 75%, DON 14 72%. The highest percentages obtained against FBs 11 94%), ZEN 17 100%), Alternaria toxins 7 96%). In conclusion, this work provided promising results production by-products, which could exploited as potential biofungicides fungi mycotoxins.

Language: Английский

Citations

8
The potential of antifungal peptides derived from Lactiplantibacillus plantarum WYH for biocontrol of Aspergillus flavus contamination DOI
Dexin Ou,

Yanyan Zou,

Xiyan Zhang

et al.

International Journal of Food Microbiology, Journal Year: 2024, Volume and Issue: 418, P. 110727 - 110727

Published: May 10, 2024

Language: Английский

Citations

3
Transcriptomic and biochemical analyses revealed antifungal mechanism of trans-anethole on Aspergillus flavus growth DOI

Jun-Dong Lei,

Qiong Li, Shuaibing Zhang

et al.

Applied Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 107(23), P. 7213 - 7230

Published: Sept. 21, 2023

Language: Английский

Citations

6
Suppression of anthracnose disease by orsellinaldehyde isolated from the mushroom Coprinus comatus DOI

Enrico M. Cabutaje,

Kotomi Ueno, Thomas Edison E. dela Cruz

et al.

Journal of Applied Microbiology, Journal Year: 2024, Volume and Issue: 135(6)

Published: May 27, 2024

Abstract Aims Anthracnose caused by Colletotrichum species is one of the most devastating diseases fruits and crops. We isolated identified an antifungal compound from mushroom Coprinus comatus investigated its inhibitory potential against anthracnose disease-causing fungi with goal discovering natural products that can suppress anthracnose-caused plant disease. Methods results The culture filtrate C. was subjected to a bioassay-guided isolation compounds. active as orsellinaldehyde (2,4-dihydroxy-6-methylbenzaldehyde) based on mass spectroscopy nuclear magnetic resonance analyses. Orsellinaldehyde displayed broad-spectrum activity different pathogenic fungi. Among tested species, it exhibited lowest IC50 values conidial germination germ tube elongation orbiculare. also showed remarkable gloeosporiodes. staining conidia fluorescein diacetate propidium iodide demonstrated fungicidal. postharvest in-vivo detached fruit assay indicated suppressed lesion symptoms mango cucumber gloeosporioides orbiculare, respectively. Conclusions potent comatus. fungicidal activities indicate fungicide for protecting various

Language: Английский

Citations

1