Carbohydrate conversion in spent coffee grounds: pretreatment strategies and novel enzymatic cocktail to produce value-added saccharides and prebiotic mannooligosaccharides

Biotechnology for Biofuels and Bioproducts, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 7, 2025

Abstract Background Spent coffee grounds (SCG) are the most abundant waste byproducts generated from beverage production worldwide. Typically, these seen as and end up in landfills. However, SCG contain valuable compounds that can be valorized used different applications. Notably, they rich carbohydrates, primarily galactomannan, arabinogalactan type II, cellulose. Within framework of a circular bioeconomy, targeted degradation polysaccharides via tailored cocktail carbohydrate-active enzymes offers promising strategy for producing high-value saccharides waste. Results In this study, various mild pretreatments were evaluated to increase enzyme accessibility SCG-derived biomass, reduce lignin content, minimize hemicellulose loss. Thermostable selected construct an enzymatic specifically targeting cellulose hemicelluloses pretreated SCGs. The approach achieved conversion 52% polysaccharide content oligo- monosaccharides, 17.4 mg reducing sugars 5.1 monosaccharides 50 SCG. Additionally, microwave pretreatment followed by application thermostable endo β-mannanase resulted 62.3 mannooligosaccharides 500 vitro experiments demonstrated produced exhibited prebiotic activity, promoting growth biofilm formation five probiotic bacterial strains. Conclusions This study highlights effective valorization through customized cocktails bioeconomic context. both oligosaccharides with activity illustrates versatility commercial potential substrate saccharides. Furthermore, use methods minimizes chemical inputs energy demands, aligning sustainable processing practices. ability selectively target degrade specific within not only enhances yield desirable products, but also preserves key structural components, resource efficiency.

Language: Английский

The Impact of Bioactive Molecules from Probiotics on Child Health: A Comprehensive Review

Nutrients, Journal Year: 2024, Volume and Issue: 16(21), P. 3706 - 3706

Published: Oct. 30, 2024

: This review investigates the impact of bioactive molecules produced by probiotics on child health, focusing their roles in modulating gut microbiota, enhancing immune function, and supporting overall development. Key metabolites, including short-chain fatty acids (SCFAs), bacteriocins, exopolysaccharides (EPSs), vitamins, gamma-aminobutyric acid (GABA), are highlighted for ability to maintain regulate inflammation, support neurodevelopment.

Language: Английский