Journal of Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12(4), P. 31 - 31
Published: Nov. 20, 2024
The
classic
model
of
sex
determination
in
insects
suggests
that
they
do
not
have
hormones
and
is
determined
a
cell-autonomous
manner.
On
the
other
hand,
there
accumulating
evidence
development
secondary
sexual
traits
controlled
non-cell-autonomous
manner
through
external
factors.
To
evaluate
degrees
regulation
trait
development,
we
analyzed
dynamics
sexually
dimorphic
transcriptome
gynandromorphic
individuals
mo
mutant
strain
silkworm
Bombyx
mori.
possesses
female
heterogametic
sex-determination
system
(ZZ
=
male/ZW
female),
where
master
regulatory
gene
for
femaleness,
Feminizer
(Fem),
located
W
chromosome.
As
trait,
focused
on
fat
body,
which
shows
remarkable
differences
between
sexes
during
last
instar
larval
stage.
A
comparison
transcriptomes
bodies
male
larvae
identified
232
sex-differentially
expressed
genes
(S-DEGs).
proportions
ZZ
ZW
cells
constituting
body
were
calculated
according
to
expression
level
Fem.
Based
obtained
values,
each
S-DEG
was
estimated,
assuming
levels
proportion
cells.
estimated
207
out
S-DEGs
strongly
correlated
with
corresponding
by
RNA-seq.
These
results
suggest
most
regulated
Journal of Clinical Laboratory Analysis,
Journal Year:
2023,
Volume and Issue:
37(3)
Published: Jan. 26, 2023
Abstract
Objective
To
explore
the
relationship
between
25(OH)D3
and
circular
RNAs
(circRNAs)
in
early
diagnosis
of
gestational
diabetes
mellitus
(GDM)
to
screen
for
biological
markers
prediction
GDM.
Methods
A
cohort
study
was
conducted
using
samples
data
collected
from
pregnant
women
registered
at
Li
Huili
hospital
China
April
2018
January
2020.
Four
circRNAs
(hsa_circ_0003218,
hsa_circ_0002968,
hsa_circ_0007430,
hsa_circ_0006260)
were
selected
as
potential
biomarkers,
quantitative
reverse
transcriptase‐polymerase
chain
reaction
(qRT‐PCR)
used
measure
their
concentration
serum
analyze
correlation
with
25(OH)D3.
The
Pearson
test
assess
25(OH)D3,
circRNAs,
various
clinical
variables.
area
under
receiver
operating
characteristic
(ROC)
curve
diagnostic
value
stage
pregnancy.
Results
Weight,
body
mass
index
(BMI),
triglycerides,
total
cholesterol,
high‐density
lipoprotein
cholesterol
(HDL‐C),
found
be
risk
factors
level
correlated
significantly
HDL‐C
a
coefficient
0.298
(
p
<
0.05).
expression
hsa_circ_0003218
downregulated
GDM
group
Hsa_circ_0002968,
hsa_circ_0006260
did
not
show
any
differential
two
groups
>
Furthermore,
0.357
AUC
combined
0.789
([0.700–0.877],
0.001),
sensitivity
specificity
63.04%
80.65%,
respectively.
Conclusions
Hsa_circ_0003218
may
jointly
participate
metabolic
process
Thus,
combination
represents
biomarker
stages
Horticulturae,
Journal Year:
2023,
Volume and Issue:
9(9), P. 962 - 962
Published: Aug. 24, 2023
The
fruitless
(fru)
gene
is
a
key
factor
in
controlling
sexual
behavior
insects.
homolog
of
fru
has
been
identified
most
insect
species
and
exhibits
conservation
the
transcript
architecture
regulation
male
behavior.
However,
structure
transcripts
Hemiptera
remains
unknown.
Here,
we
characterized
two
Bemisia
tabaci,
named
fru-a
fru-b.
contained
1263
bp
open
reading
frame
(ORF)
encoding
for
420
amino
acids,
while
fru-b
1143
ORF
380
acids.
These
proteins
start
with
same
BTB
domain
end
different
zinc
finger
domains,
belonging
to
ZnA
ZnG,
respectively.
expression
differed
significantly
between
females
males,
both
were
expressed
at
lower
levels
males.
We
demonstrated
presence
multiple
TRA/TRA-2
binding
sites
alternative
splicing
Moreover,
RNAi
result
provided
evidence
that
transformer
regulates
results
promote
study
sex
determination
cascade
B.
tabaci
lay
foundation
this
insect.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2021,
Volume and Issue:
unknown
Published: May 14, 2021
Abstract
Sexual
dimorphism
is
common
in
animals.
The
most
complete
model
of
sex
determination
comes
from
Drosophila
melanogaster
,
where
the
relative
dosage
autosomes
and
X
chromosomes
leads
indirectly
to
sex-specific
transcripts
doublesex
.
Female
Dsx
interacts
with
a
mediator
complex
protein
encoded
by
intersex
activate
female
development.
In
males
transcription
factor
fruitless
promotes
male-specific
behavior.
genetics
have
been
examined
small
number
other
insects,
yet
several
questions
remain
about
pleisomorphic
state.
Is
required
for
male
development?
conserved
behavior
or
morphology?
Are
components
such
as
functionally
conserved?
To
address
these
questions,
we
report
expression
functional
tests
doublesex,
hemipteran
Oncopeltus
fasciatus
characterizing
three
sexual
dimorphisms.
prevents
phenotypes
all
sexes
dimorphic
traits
milkweed
bug.
are
expressed
across
body,
females
males.
also
affect
genitalia
both
sexes,
but
effects
limited
different
structures
sexes.
These
results
reveal
roles
distinct
demonstrate
development
mechanisms
sexually
structures.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 8, 2024
ABSTRACT
The
molecular
mechanism
of
sex
determination
has
long
been
considered
conserved
in
insects.
However,
recent
studies
hemimetabolous
insects
have
challenged
this
notion.
One
notable
example
is
termites.
In
Reticulitermes
speratus
,
a
homolog
gene,
Doublesex
(
RsDsx
),
exhibits
characteristics
that
are
distinct
from
those
other
insects,
including
sister-group
cockroaches.
It
comprises
single
exon,
contains
only
doublesex/mab-3
DNA-binding
domain
(DM)
but
lacks
oligomerization
(OD),
and
transcriptional
activity
males.
To
investigate
whether
these
widespread
within
the
termite
lineage,
we
identified
Dsx
homologs
three
different
families.
loss
OD
sequences
was
observed
all
species
examined,
whereas
number
exons
expression
patterns
between
sexes
varied
among
Particularly,
distinctive
differences
were
found
Archotermopsidae
Kalotermitidae,
both
which
linear
caste
developmental
pathway.
Our
findings
indicate
diversification
structure
may
contributed
to
ecological
diversification,
such
as
pathways,
lineage.
Journal of Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12(4), P. 31 - 31
Published: Nov. 20, 2024
The
classic
model
of
sex
determination
in
insects
suggests
that
they
do
not
have
hormones
and
is
determined
a
cell-autonomous
manner.
On
the
other
hand,
there
accumulating
evidence
development
secondary
sexual
traits
controlled
non-cell-autonomous
manner
through
external
factors.
To
evaluate
degrees
regulation
trait
development,
we
analyzed
dynamics
sexually
dimorphic
transcriptome
gynandromorphic
individuals
mo
mutant
strain
silkworm
Bombyx
mori.
possesses
female
heterogametic
sex-determination
system
(ZZ
=
male/ZW
female),
where
master
regulatory
gene
for
femaleness,
Feminizer
(Fem),
located
W
chromosome.
As
trait,
focused
on
fat
body,
which
shows
remarkable
differences
between
sexes
during
last
instar
larval
stage.
A
comparison
transcriptomes
bodies
male
larvae
identified
232
sex-differentially
expressed
genes
(S-DEGs).
proportions
ZZ
ZW
cells
constituting
body
were
calculated
according
to
expression
level
Fem.
Based
obtained
values,
each
S-DEG
was
estimated,
assuming
levels
proportion
cells.
estimated
207
out
S-DEGs
strongly
correlated
with
corresponding
by
RNA-seq.
These
results
suggest
most
regulated
