Adipokinetic Hormones and Their Receptor Regulate the Locomotor Behavior in Tribolium castaneum DOI Creative Commons

Roujian Lu,

Xiaochuan Pang,

S H Wen

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 407 - 407

Published: April 12, 2025

The regulation of locomotor behavior is essential for insects to perform their life activities. central nervous system plays a pivotal role in modulating physiological behaviors, particularly movement, with neuropeptides serving as key modulators these processes. Among these, adipokinetic hormone (AKH) was originally identified neurohormone involved lipid mobilization. This study investigates the functional AKHs (AKH1 and AKH2) receptor (AKHR) regulating locomotion red flour beetle, Tribolium castaneum. Using calcium reporter assays, we demonstrated that AKHR activated by two mature AKH peptides from T. castaneum, half-maximal effective concentrations (EC50) falling within nanomolar range. Gene expression analysis confirmed presence AKH1 AKH2 transcripts brain, while localized fat body carcass. silencing or through RNA interference resulted significant reduction both movement distance duration. Collectively, findings highlight regulatory influence AKH/AKHR signaling activity thereby advancing our understanding molecular mechanisms underlying control this economically important insect species.

Language: Английский

Adipokinetic Hormones and Their Receptor Regulate the Locomotor Behavior in Tribolium castaneum DOI Creative Commons

Roujian Lu,

Xiaochuan Pang,

S H Wen

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 407 - 407

Published: April 12, 2025

The regulation of locomotor behavior is essential for insects to perform their life activities. central nervous system plays a pivotal role in modulating physiological behaviors, particularly movement, with neuropeptides serving as key modulators these processes. Among these, adipokinetic hormone (AKH) was originally identified neurohormone involved lipid mobilization. This study investigates the functional AKHs (AKH1 and AKH2) receptor (AKHR) regulating locomotion red flour beetle, Tribolium castaneum. Using calcium reporter assays, we demonstrated that AKHR activated by two mature AKH peptides from T. castaneum, half-maximal effective concentrations (EC50) falling within nanomolar range. Gene expression analysis confirmed presence AKH1 AKH2 transcripts brain, while localized fat body carcass. silencing or through RNA interference resulted significant reduction both movement distance duration. Collectively, findings highlight regulatory influence AKH/AKHR signaling activity thereby advancing our understanding molecular mechanisms underlying control this economically important insect species.

Language: Английский

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