Comparative time-series analyses of gut microbiome profiles in genetically and chemically induced lupus-prone mice and the impacts of fecal transplantation DOI Creative Commons

Piraya Chatthanathon,

Asada Leelahavanichkul,

Thanya Cheibchalard

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 2, 2024

Although the association between gut dysbiosis (imbalance of microbiota) in systemic lupus erythematosus (SLE) is well-known, simultaneous exploration fecal and different intestinal sections before after onset (at 2, 4, 6, 8, 10 months old) resulting from loss inhibitory Fc gamma receptor IIb (FcGIIb) pristane induction have never been conducted. Anti-dsDNA (an important autoantibody) proteinuria developed as early 6 old both models, with higher levels FcGRIIb deficient (FcGRIIb-/-) mice. Compared to healthy control at 2 4 months, mice (both FcGRRIIb-/- pristane) demonstrated an alteration indicated by Shannon alpha diversity index, highlighting influences lupus- age-induced dysbiosis, respectively. Non-metric multidimensional scaling (NMDS) revealed that microbiota FcGRIIb-/- were distinct age-matched all timepoints month, p < 0.05), while showed divergence only some timepoints. Analyses similarity among cecum, colon, feces, contrasting those small intestines (duodenum, jejunum, ileum). Subtle differences found feces assessed several analyses, for examples, similar or dissimilar distances (NMDS), neighbor-joining clustering, potential metabolisms (KEGG pathway analysis). Due (feces sections) control, rebalancing using rectal administration (fecal transplantation; FMT) 7-month-old FcGIIb-/- (the established lupus; positive anti-dsDNA proteinuria) was performed. In comparison without FMT, FMT (more effect on female than male mice) lower microbiome (16s DNA gene copy number) patterns control. conclusion, (FcGRIIb-/- diverged 4-6 old, correlating characteristics (anti-dsDNA proteinuria). The suggested a possible various molecular causes. Furthermore, appeared mitigate reduce anti-dsDNA, supporting benefit lupus, more studies are warranted.

Language: Английский

A Comprehensive Review of Fc Gamma Receptors and Their Role in Systemic Lupus Erythematosus DOI Open Access
Jesús Sepúlveda-Delgado,

Luis Enrique Montiel Llorente,

Susana Hernández-Doño

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1851 - 1851

Published: Feb. 21, 2025

Receptors for the immunoglobulin G constant fraction (FcγRs) are widely expressed in cells of immune system. Complement-independent phagocytosis prompted FcγR research to show that engagement IgG complexes with FcγRs triggers a variety cell host responses, such as phagocytosis, antibody-dependent cytotoxicity, and NETosis, among others. However, variants these receptors have been implicated development susceptibility autoimmune diseases systemic lupus erythematosus. Currently, knowledge is required field antibody therapeutics, which includes engineering recombinant soluble human Fc gamma receptors, enhancing inhibitory blocking activating function, vaccines, organ transplantation. Importantly, recent interest enhancement (ADE), mechanism by pathogenesis certain viral infections enhanced. ADEs may be responsible severity SARS-CoV-2 infection. Therefore, become current topic. this review briefly describes some historical about type I family humans, including structure, affinity, ligand binding, erythematosus (SLE), potential therapeutic approaches related SLE.

Language: Английский

Citations

1
Aging-induced dysbiosis worsens sepsis severity but is attenuated by probiotics in D-galactose-administered mice with cecal ligation and puncture model DOI Creative Commons

Chalisa Pinitchun,

Wimonrat Panpetch,

Thansita Bhunyakarnjanarat

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(10), P. e0311774 - e0311774

Published: Oct. 18, 2024

Introduction Despite the well-established effects of aging on brain function and gut dysbiosis (an imbalance in microbiota), influence sepsis-associated encephalopathy (SAE) role probiotics this context remain less understood. Methods C57BL/6J mice (8-week-old) were subcutaneously administered with 8 weeks D-galactose (D-gal) or phosphate buffer solution (PBS) for non-aging models, respectively, without oral Lacticaseibacillus rhamnosus GG (LGG). Additionally, impact condition media from LGG (LCM) was tested macrophages (RAW 264.7 cells), microglia (BV-2 hippocampal cells (HT-22 cells). Result Fecal microbiome analysis demonstrated D-gal-induced (reduced Firmicutes Desulfobacterota increased Bacteroidota Verrucomicrobiota), which partially neutralized dysbiosis. D-gal also worsens cecal ligation puncture (CLP) sepsis severity when compared PBS-CLP mice, as indicated by serum creatinine (Scr) alanine transaminase (ALT), but not mortality, neurological characteristics (SHIRPA score), cytokines (TNF-α IL-6). supported fibrosis liver, kidney, lung; however, CLP did worsen fibrosis. Interestingly, attenuated all parameters (mortality, Scr, ALT, SHIRPA, cytokines) (PBS-CLP) while improving these parameters, except mortality IL-6, (D-gal CLP). For vitro test using lipopolysaccharide (LPS) stimulation, LCM inflammation some RAW264.7 BV-2 HT-22 cells, implying a direct anti-inflammatory effect macrophages, brain. Conclusion induced fecal worsened determined Scr could alleviate most selected sepsis, including SAE. However, SAE delivery beneficial molecules to partly due attenuation systemic through modulation macrophages.

Language: Английский

Citations

5
Intratracheal Candida administration induced lung dysbiosis, activated neutrophils, and worsened lung hemorrhage in pristane-induced lupus mice DOI Creative Commons

Thansita Bhunyakarnjanarat,

Kanyarat Udompornpitak, Dhammika Leshan Wannigama

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 21, 2025

Because the innate immunity might and fungi in lungs enhance severity of lupus-induced diffuse alveolar hemorrhage (DAH), intraperitoneal pristane injection was performed C57BL6 mice with intratracheal administration by Candida albicans or phosphate buffer solution (PBS). Despite similar pristane-induced lupus (proteinuria, serum creatinine, anti-dsDNA) at 5 weeks model, worsened several characteristics, including mortality, body weight, cytokines (TNF-α IL-6), lung score, tissue (TNF-α, IL-6, IL-10), but not gut permeability (FITC-dextran assay), IL-10, immune cells spleens (flow cytometry analysis), activities peritoneal macrophages (polymerase-chain reaction). Although reduced proteobacterial abundance altered alpha beta diversity compared PBS control, microbiota different between pristane- non-pristane-administered mice. prominent Gram-negative bacteria role neutrophils DAH, lipopolysaccharide (LPS) without heat-killed preparation tested. Indeed, LPS induced more severe pro-inflammatory than stimulation alone as indicated expression genes IL-1β, Dectin-1, NF-κB). In conclusion, partly through enhanced neutrophil responses against fungi. More studies on colonization sputum from patients DAH are interesting.

Language: Английский

Citations

0
Alcohol-induced gut permeability defect through dysbiosis and enterocytic mitochondrial interference causing pro-inflammatory macrophages in a dose dependent manner DOI Creative Commons
Wiwat Chancharoenthana, Supitcha Kamolratanakul, Kanyarat Udompornpitak

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 27, 2025

Although toxicity of alcohol toward the intestines and immunity is mentioned, there might be different effect in a low high dose rodent model development using simple SHIRPA binary score night useful. Hence, (6.30 1.26 g/kg/day) were administered for 16 weeks before determination several parameters. As such, peak blood concentration (BAC) approximately at 0.05 0.15%, respectively, 1 h post-administration, which correlated with 1.8 ± 0.8 7.2 0.6, respectively. After wk administration, significant liver injury high-dose was indicated by enzymes, weight, histology score, apoptosis, hepatic accumulation triglyceride (TG) oxidative stress (malondialdehyde; MDA) reduced anti-oxidant (glutathione). Meanwhile, low-dose demonstrated only elevated apoptosis increased TG MDA tissue. Leaky gut from both also FITC-dextran, endotoxemia, serum beta glucan, occludin. However, bacterial abundance (microbiome analysis) feces small bowel alcohol, but not dose, control (increased Alitipes spp. Lachnospiraceae). In conclusion, low- induced leaky gut, while caused dysbiosis damaged mitochondria enhanced glycolysis enterocytes macrophages. more sensitive than to determine alcohol-induced intestinal injury.

Language: Английский

Citations

0
Accelerating and protective effects toward cancer growth in cGAS and FcgRIIb deficient mice, respectively, an impact of macrophage polarization DOI
Arthid Thim-uam,

Papasara Chantawichitwong,

Pornpimol Phuengmaung

et al.

Inflammation Research, Journal Year: 2025, Volume and Issue: 74(1)

Published: April 24, 2025

Language: Английский

Citations

0
Alterations of the gut microbiota in the lupus nephritis: a systematic review DOI Creative Commons

Anjing Wang,

Jin Zhao, Yunlong Qin

et al.

Renal Failure, Journal Year: 2023, Volume and Issue: 45(2)

Published: Nov. 23, 2023

Background Emerging evidence suggests that gut microbiota dysbiosis may play a critical role in the development of lupus nephritis (LN). However, specific characteristics individuals with LN have not been fully clarified.

Language: Английский

Citations

7
Comparative time-series analyses of gut microbiome profiles in genetically and chemically induced lupus-prone mice and the impacts of fecal transplantation DOI Creative Commons

Piraya Chatthanathon,

Asada Leelahavanichkul,

Thanya Cheibchalard

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 2, 2024

Although the association between gut dysbiosis (imbalance of microbiota) in systemic lupus erythematosus (SLE) is well-known, simultaneous exploration fecal and different intestinal sections before after onset (at 2, 4, 6, 8, 10 months old) resulting from loss inhibitory Fc gamma receptor IIb (FcGIIb) pristane induction have never been conducted. Anti-dsDNA (an important autoantibody) proteinuria developed as early 6 old both models, with higher levels FcGRIIb deficient (FcGRIIb-/-) mice. Compared to healthy control at 2 4 months, mice (both FcGRRIIb-/- pristane) demonstrated an alteration indicated by Shannon alpha diversity index, highlighting influences lupus- age-induced dysbiosis, respectively. Non-metric multidimensional scaling (NMDS) revealed that microbiota FcGRIIb-/- were distinct age-matched all timepoints month, p < 0.05), while showed divergence only some timepoints. Analyses similarity among cecum, colon, feces, contrasting those small intestines (duodenum, jejunum, ileum). Subtle differences found feces assessed several analyses, for examples, similar or dissimilar distances (NMDS), neighbor-joining clustering, potential metabolisms (KEGG pathway analysis). Due (feces sections) control, rebalancing using rectal administration (fecal transplantation; FMT) 7-month-old FcGIIb-/- (the established lupus; positive anti-dsDNA proteinuria) was performed. In comparison without FMT, FMT (more effect on female than male mice) lower microbiome (16s DNA gene copy number) patterns control. conclusion, (FcGRIIb-/- diverged 4-6 old, correlating characteristics (anti-dsDNA proteinuria). The suggested a possible various molecular causes. Furthermore, appeared mitigate reduce anti-dsDNA, supporting benefit lupus, more studies are warranted.

Language: Английский

Citations

2