Africanized honeybee venom (Apis mellifera) promotes human complement activation split products storm DOI Creative Commons
Felipe Silva de França, Ricardo de Oliveira Orsi, Dayanne Carla Fernandes

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 13, 2024

Introduction Complement activation split products are signatures of many immunopathological disorders. Among the laboratory findings observed in these diseases, a reduction level circulating intact complement components can be mentioned, and this change has also been detected envenomation by multiple Africanized honeybee (Apis mellifera) stings. Although animals elicits diverse life-threatening reactions, capacity bee venom (AmV) to activate human system remains elusive. Methods By coupling immunochemical functional approaches, it was that AmV strongly consumes alternative pathway (AP) normal serum (NHS). Additionally, interfered with classical (CP) lectin pathways (LP) activities. In parallel, high increase Ba fragment levels detected, suggesting changes AP activity were due its activation. Furthermore, an C1s-C1INH complex decrease physiological MASP1-C1INH suggested CP LP activated presence AmV. Strikingly, NHS exposed increasing concentrations varying from 5 1000 µg/mL presented generation C3a, C4a C5a anaphylatoxins, sC5b-9 complexes assembly, thus reinforcing triggers Conclusion These results show is strong activator. This presents mixed profile, predominance suggests play important roles honeybee, as they could induce events patients may dictate patient clinical prognosis.

Language: Английский

Decreased plasma cartilage acidic protein 1 in COVID‐19 DOI Creative Commons
Mats W. Johansson, Joseph Balnis, Laura K. Muehlbauer

et al.

Physiological Reports, Journal Year: 2023, Volume and Issue: 11(17)

Published: Sept. 1, 2023

Abstract Cartilage acidic protein‐1 (CRTAC1) is produced by several cell types, including Type 2 alveolar epithelial (T2AE) cells that are targeted SARS‐CoV2. Plasma CRTAC1 known based on proteomic surveys to be low in patients with severe COVID‐19. Using an ELISA, we found treated for COVID‐19 ICU almost uniformly had plasma concentrations of below those healthy controls. Magnitude decrease distinguished from other causes acute respiratory decompensation and correlated established metrics severity. controls were some a year after hospitalization COVID‐19, long COVID less or chronic obstructive pulmonary disease. Decreases ( r = 0.37, p 0.0001) decreases CFP (properdin), which interacts CRTAC1. Thus, associated may result loss production T2AE co‐depletion CFP. Determination significance reasons behind decreased concentration subset will require analysis roles preexisting lung disease, impact prior age, confounding variables larger number patients.

Language: Английский

Citations

1
A predominately pulmonary activation of complement in a mouse model of severe COVID-19 DOI Creative Commons
Peter J. Szachowicz,

Christine Wohlford-Lenane,

Cobey J. Heinen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 3, 2024

Evidence from in vitro studies and observational human disease data suggest the complement system plays a significant role SARS-CoV-2 pathogenesis, although how dysregulation develops patients with severe COVID-19 is unknown. Here, using mouse-adapted virus (SARS2-N501Y

Language: Английский

Citations

0
Complement is Primarily Activated in the Lung in a Severe COVID-19 Mouse Model DOI
Peter J. Szachowicz,

Christine Wohlford-Lenane,

Cobey J. Heinen

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Long COVID: current management and future prospects DOI
Felicity Liew, Peter Openshaw

European Respiratory Society eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 27, 2024

Language: Английский

Citations

0
Africanized honeybee venom (Apis mellifera) promotes human complement activation split products storm DOI Creative Commons
Felipe Silva de França, Ricardo de Oliveira Orsi, Dayanne Carla Fernandes

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 13, 2024

Introduction Complement activation split products are signatures of many immunopathological disorders. Among the laboratory findings observed in these diseases, a reduction level circulating intact complement components can be mentioned, and this change has also been detected envenomation by multiple Africanized honeybee (Apis mellifera) stings. Although animals elicits diverse life-threatening reactions, capacity bee venom (AmV) to activate human system remains elusive. Methods By coupling immunochemical functional approaches, it was that AmV strongly consumes alternative pathway (AP) normal serum (NHS). Additionally, interfered with classical (CP) lectin pathways (LP) activities. In parallel, high increase Ba fragment levels detected, suggesting changes AP activity were due its activation. Furthermore, an C1s-C1INH complex decrease physiological MASP1-C1INH suggested CP LP activated presence AmV. Strikingly, NHS exposed increasing concentrations varying from 5 1000 µg/mL presented generation C3a, C4a C5a anaphylatoxins, sC5b-9 complexes assembly, thus reinforcing triggers Conclusion These results show is strong activator. This presents mixed profile, predominance suggests play important roles honeybee, as they could induce events patients may dictate patient clinical prognosis.

Language: Английский

Citations

0