Eldecalcitol alleviates diabetic periodontitis by regulating macrophage efferocytosis and polarization via SOCE machinery DOI Creative Commons

H. Liu,

Changyun Sun, Yujun Jiang

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113894 - 113894

Published: Dec. 26, 2024

Diabetes exacerbates the occurrence and severity of periodontitis, pathogenesis diabetic periodontitis (DPD) is influenced by delayed resolution inflammation. Eldecalcitol (ED-71) has shown promise in preventing bone loss DPD. We herein aimed to investigate role ED-71 inflammatory regression phase DPD elucidate underlying mechanisms. Type-2 diabetes was induced streptozotocin injection Wistar rats, explore vivo effect on macrophage efferocytosis, ligation combined with lipopolysaccharide. Alveolar destruction assessed using micro-computed tomography, hematoxylin-eosin, immunohistochemistry, tartrate-resistant acid phosphatase staining. Immunofluorescence staining flow cytometry detected neutrophils, apoptotic cells, polarization periodontal tissue. Additionally, cytometry, real-time polymerase chain reaction, western blotting, enzyme-linked immunosorbent assay were used examine efferocytosis changes store-operated calcium entry (SOCE). found that rats exhibited more severe alveolar increased neutrophil aggregates Following administration, significantly decreased, immune microenvironment tissue tended suppress Macrophages stimulated high glucose experienced disruption SOCE machinery, leading inhibition vitro. demonstrated ability restore correcting SOCE, sustained damage In conclusion, impairs M2 resulting could attenuate mitigating via machinery

Language: Английский

Eldecalcitol alleviates diabetic periodontitis by regulating macrophage efferocytosis and polarization via SOCE machinery DOI Creative Commons

H. Liu,

Changyun Sun, Yujun Jiang

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113894 - 113894

Published: Dec. 26, 2024

Diabetes exacerbates the occurrence and severity of periodontitis, pathogenesis diabetic periodontitis (DPD) is influenced by delayed resolution inflammation. Eldecalcitol (ED-71) has shown promise in preventing bone loss DPD. We herein aimed to investigate role ED-71 inflammatory regression phase DPD elucidate underlying mechanisms. Type-2 diabetes was induced streptozotocin injection Wistar rats, explore vivo effect on macrophage efferocytosis, ligation combined with lipopolysaccharide. Alveolar destruction assessed using micro-computed tomography, hematoxylin-eosin, immunohistochemistry, tartrate-resistant acid phosphatase staining. Immunofluorescence staining flow cytometry detected neutrophils, apoptotic cells, polarization periodontal tissue. Additionally, cytometry, real-time polymerase chain reaction, western blotting, enzyme-linked immunosorbent assay were used examine efferocytosis changes store-operated calcium entry (SOCE). found that rats exhibited more severe alveolar increased neutrophil aggregates Following administration, significantly decreased, immune microenvironment tissue tended suppress Macrophages stimulated high glucose experienced disruption SOCE machinery, leading inhibition vitro. demonstrated ability restore correcting SOCE, sustained damage In conclusion, impairs M2 resulting could attenuate mitigating via machinery

Language: Английский

Citations

1