Evaluation of wound temperature monitoring at various anatomical sites in the management of patients with diabetic foot undergoing microcirculation reconstruction DOI Creative Commons

Hong Liu,

Xian‐Yan Yan,

Guoqing Li

et al.

Journal of Orthopaedic Surgery and Research, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 20, 2024

This study aims to assess the significance of monitoring temperature change trends at various wound sites in healing process diabetic foot ulcers after microcirculation reconstruction surgery. A retrospective analysis was conducted on individuals with who had been admitted Department Orthopedics Second Hospital Shanxi Medical University between July 2020 and February 2022. Temperature changes were regularly monitored center distal tibia ipsilateral lower leg microcirculatory blood perfusion. Wound, ischemia, infection (WIFi) grading performed admission final follow-up determine value sites. Additionally, formation collateral microarterial vessels their consistency observed differences. Follow-up assessments included recurrence ulcers, development different locations, re-amputation toe or limb, diabetes-related mortality. total 29 patients follow-up, an average age 57.14 ± 14.75 years a period 9.79 4.13 months. Following surgery, as microvascular network formed, difference same side gradually decreased, no statistical 4 weeks postoperatively. At both there significant reduction (W) ischemia (I) grades within WIFi classification. The showed progressive improvement developed. During period, 2 cases ulcer recurrence, 1 case appearing location, fatalities. Skin offers direct reliable indication Its simplicity cost-effectiveness make it valuable tool for widespread use evaluating following

Language: Английский

Thou shall not heal: Overcoming the non-healing behaviour of diabetic foot ulcers by engineering the inflammatory microenvironment DOI Creative Commons
Michael G. Monaghan, Rajiv Borah,

Charlotte Thomsen

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 203, P. 115120 - 115120

Published: Oct. 25, 2023

Diabetic foot ulcers (DFUs) are a devastating complication for diabetic patients that have debilitating effects and can ultimately lead to limb amputation. Healthy wounds progress through the phases of healing leading tissue regeneration restoration barrier function skin. In contrast, in dysregulation these leads chronic, non-healing wounds. particular, unresolved inflammation DFU microenvironment has been identified as key facet chronic hyperglyceamic patients, DFUs fail beyond inflammatory phase towards resolution. Thus, control over modulation response is promising therapeutic avenue treatment. This review discusses current state-of-the art regarding microenvironment, with specific focus on development biomaterials-based delivery strategies their cargos direct microenvironment.

Language: Английский

Citations

40
Preclinical study of diabetic foot ulcers: From pathogenesis to vivo/vitro models and clinical therapeutic transformation DOI Creative Commons
Yuqing Du, Jie Wang, Weijing Fan

et al.

International Wound Journal, Journal Year: 2023, Volume and Issue: 20(10), P. 4394 - 4409

Published: July 12, 2023

Abstract Diabetic foot ulcer (DFU), a common intractable chronic complication of diabetes mellitus (DM), has prevalence up to 25%, with more than 17% the affected patients at risk amputation or even death. Vascular factors, including vascular stenosis occlusion, dyslipidemia, impaired neurosensory and motor function, skin infection caused by trauma, all increase DFU in diabetes. Therefore, diabetic is not single pathogenesis. Preclinical studies have contributed greatly pathogenesis determination efficacy evaluation DFU. Many therapeutic tools are currently being investigated using animal models for effective clinical translation. However, preclinical that completely mimic remain unexplored. this review, preparation methods criteria three major pathological mechanisms: neuropathy, angiopathy were discussed detail. And advantages disadvantages various sign simulation. Furthermore, current status vitro some been transformed into treatment programs, such as medical dressings, growth factor therapy, 3D bioprinting pre‐vascularization, Traditional Chinese Medicine treatment. because complexity mechanism DFU, transformation model still faces many challenges. We need further optimize existing provide an platform future study pathophysiology

Language: Английский

Citations

14
Identifying and Validating GSTM5 as an Immunogenic Gene in Diabetic Foot Ulcer Using Bioinformatics and Machine Learning DOI Creative Commons
Hongshuo Shi, Xin Yuan, Guobin Liu

et al.

Journal of Inflammation Research, Journal Year: 2023, Volume and Issue: Volume 16, P. 6241 - 6256

Published: Dec. 1, 2023

A diabetic foot ulcer (DFU) is a serious, long-term condition associated with significant risk of disability and mortality. However, research on its biomarkers still limited. This study utilizes bioinformatics machine learning methods to identify immune-related for DFU validates them through external datasets animal experiments.This used analyze microarray data from the Gene Expression Omnibus (GEO) database key genes DFU. Animal experiments were conducted validate these findings. employs GSE68183 GSE80178 retrieved GEO as training dataset building gene model, after conducting differential analysis data, this package glmnet e1071 construct LASSO SVM-RFE models, respectively. Subsequently, we validated model using set validation (GSE134431). We enrichment analysis, including GSEA GSVA, genes. also performed immune functional Finally, immunohistochemistry (IHC) genes.This identifies GSTM5 potential target in methods. Subsequent IHC confirms critical biomarker The may be T cells regulatory (Tregs) follicular helper, it influences NF-κB, GnRH, MAPK signaling pathway.This identified finding potentially provide therapy

Language: Английский

Citations

11
Optimizing risk management for post-amputation wound complications in diabetic patients: Focus on glycemic and immunosuppressive control DOI
Zhipeng Li,

Jin-Ke Sun,

Weiping Fu

et al.

World Journal of Diabetes, Journal Year: 2025, Volume and Issue: 16(3)

Published: Jan. 20, 2025

This study highlights the importance of identifying and addressing risk factors associated with wound complications following transtibial amputation in diabetic patients. These amputations, often necessitated by severe foot ulcers, carry significant risks postoperative such as infection delayed healing. Elevated hemoglobin A1c levels, indicative poor glycemic control, a history kidney transplantation, due to required immunosuppressive therapy, are key influencing these outcomes. paper emphasizes need for enhanced management personalized care, particularly immunocompromised individuals, minimize improve patient prognosis. Future research should focus on prospective studies validate targeted interventions optimize care strategies, ultimately aiming reduce healthcare burden complications.

Language: Английский

Citations

0
Elaboration and verification of immune-based diagnostic biomarker panel for diabetic foot ulcer DOI
Huiying Gao,

Sibing Chen,

Jiannan Li

et al.

Journal of Diabetes and its Complications, Journal Year: 2025, Volume and Issue: unknown, P. 108957 - 108957

Published: Jan. 1, 2025

Language: Английский

Citations

0
The landscape of biofilm models for phage therapy: mimicking biofilms in diabetic foot ulcers using 3D models DOI Creative Commons
Mark Grevsen Martinet, Marvin L. Thomas,

Jörg Bojunga

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 24, 2025

Diabetic foot ulcers (DFU) affect up to 15-25% of patients suffering from diabetes and are considered a global health concern. These may result in delayed wound healing chronic infections, with the potential lead amputations. It has been estimated that 85% diabetes-related amputations preceded by diagnosis DFU. A critical factor persistence this disease is presence polymicrobial biofilms, which generally include Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli. The involvement diabetic comorbidities such as ischemia, hyperglycemia, immune-compromised status creates perfect niche for these bacteria evade body's immune response persist biofilms. Bacteriophage therapy can target lyse specific emerging an effective treatment biofilm-related infections. While shows promise addressing wounds, our current models, including animal static systems, fail capture full complexity Innovative approaches 3D bioengineered skin organoid hydrogel-based systems being developed simulate DFU microenvironments more accurately without using ex vivo or tissues. advanced models evaluating bacteriophage efficacy biofilm-associated DFU, aiming enhance preclinical assessments improve therapeutic outcomes patients.

Language: Английский

Citations

0
Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers DOI Creative Commons
Songyun Zhao, Hua Yu, Zihao Li

et al.

Biology Direct, Journal Year: 2025, Volume and Issue: 20(1)

Published: March 22, 2025

Diabetic foot ulcers (DFU) are a common and severe complication among diabetic patients, posing significant burden on patients' quality of life healthcare systems due to their high incidence, amputation rates, mortality. This study utilized single-cell RNA sequencing technology deeply analyze the cellular heterogeneity skin feet ofDFU patients transcriptomic characteristics endothelial cells, aiming identify key cell populations genes associated with healing progression DFU. The found that cells from DFU exhibited differences under various conditions, particularly in signaling pathways related inflammatory responses angiogenesis. Through trajectory analysis communication research, we revealed role subsets development identified multiple important gene modules Notably, promoting effect SH3BGRL3 proliferation, migration, angiogenic capabilities glucose conditions was experimentally verified, providing new potential target theoretical basis for treatment not only enhances understanding pathogenesis but also provides scientific ofnew therapeutic strategies.

Language: Английский

Citations

0
From Nature to Healing: How Gynura cusimbua May Support Diabetic Wound Recovery DOI
Nongmaithem Randhoni Chanu, Kunal Bhattacharya, Saikat Sen

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106544 - 106544

Published: April 1, 2025

Language: Английский

Citations

0
Phage therapy: a promising approach for Staphylococcus aureus diabetic foot infections DOI Creative Commons
Lucile Plumet, Chloé Magnan, Denis Costechareyre

et al.

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

ABSTRACT Diabetic foot infections (DFIs), predominantly caused by Staphylococcus aureus , pose a significant healthcare challenge with severe consequences, including amputation. Phage therapy, which utilizes bacteriophages to specifically target bacterial pathogens, has emerged as promising alternative conventional antibiotic treatments. This review evaluates the efficacy of phage therapy complementary treatment for DFIs S. synthesizing evidence from preclinical and clinical studies while addressing limitations challenges associated current research. The analysis highlights results diabetic animal models, demonstrating effective load reduction improved wound healing. Clinical case reports series further underline improvements in infection management ulcer healing, no major adverse effects reported. Ongoing trials are also discussed, offering insights into study parameters evaluating potential safety -related DFIs. While collected data highlight valuable complement traditional treatments, particularly managing antibiotic-resistant infections, research is essential address existing limitations, gaps long-term standardization. With continued investigation, holds alleviate burden improve patient outcomes.

Language: Английский

Citations

0
Immunomodulation of Macrophages in Diabetic Wound Individuals by Structurally Diverse Bioactive Phytochemicals DOI Creative Commons
Krishnendu Adhikary, Riya Sarkar,

Sriparna Maity

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(10), P. 1294 - 1294

Published: Sept. 28, 2024

Diabetes-related ulcers and slow-healing wounds pose a significant health risk to individuals due their uncertain causes. Mortality rates for diabetes foot (DFUs) range from 10% after 16 months 24% five years. The use of bioactive phytochemicals can play key role in healing predictable time. Recent literature has demonstrated that various natural substances, including flavonoids, saponins, phenolic compounds, polysaccharides, roles at different stages the wound-healing process through diverse mechanisms. These studies have categorized compounds according characteristics, bioactivities, modes action. In this study, we evaluated derived plant sources been shown crucial immunomodulation. Macrophages are closely involved immunomodulation within wound microenvironment players efferocytosis, inflammation resolution, tissue regeneration, all which contribute successful healing. Phytochemicals derivatives capabilities immune regulation, macrophage migration, nitric oxide synthase inhibition, lymphocyte T-cell stimulation, cytokine activation, killer cell enhancement, regulation NF-κβ, TNF-α, apoptosis. review, studied resolution diabetic inflammation.

Language: Английский

Citations

3