Multiple strain analysis of Streptomyces species from Philippine marine sediments reveals intraspecies heterogeneity in antibiotic activities

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Sept. 2, 2021

Abstract The marine ecosystem has become the hotspot for finding antibiotic-producing actinomycetes across globe. Although marine-derived display strain-level genomic and chemodiversity, it is unclear whether functional traits, i.e ., antibiotic activity, vary in near-identical Streptomyces species. Here, we report culture-dependent isolation, phylogeny, biodiversity, abundance, distribution of isolated from sediments west-central Philippines. Out 2212 sediment-derived actinomycete strains 11 geographical sites, 92 exhibited antibacterial activities against multidrug-resistant Staphylococcus aureus , Pseudomonas aeruginosa Escherichia coli . 16S rRNA rpoB gene sequence analyses confirmed that belong to genus highlighting parvulus as most dominant species three possible new Antibiotic-producing were highly diverse Southern Antique, diversity increase with sediment depth. Multiple sequences displayed varying strength activities. genotyping PKS NRPS genes revealed closely related have similar BGC domains supported by their close phylogenetic proximity. These findings collectively suggest ' intraspecies adaptive characteristics distinct ecological niches resulted outcompeting other bacteria through differential production.

Language: Английский

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Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes

Antibiotics, Journal Year: 2025, Volume and Issue: 14(1), P. 108 - 108

Published: Jan. 20, 2025

Background: The increasing prevalence of drug-resistant tuberculosis (TB) underscores the urgent need for novel antimicrobial agents. Methods: This study integrates cultivation optimization, nuclear magnetic resonance (NMR) fingerprinting, and principal component analysis (PCA) to explore microbial secondary metabolites as potential anti-TB Results: Using combined approach, 11 bioactive compounds were isolated identified, all exhibiting anti-Mycobacterium bovis BCG activity. Notable findings include borrelidin, a potent threonyl-tRNA synthetase inhibitor with broad biological activities, L-O-Lac-L-Val-D-O-Hiv-D-Val, peptide first time from plant endophyte, demonstrating broad-spectrum Additionally, elaiophylin polycyclic tetramate macrolactams (PTMs) displayed significant bactericidal effects, achieving complete inhibition at 72 h PTMs marking their reported also identified bafilomycins scaffolds drug development, showcasing rapid activity low MIC values. Conclusions: These emphasize value reservoir provide new avenues developing next-generation therapies.

Language: Английский

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Tolerance to NSAIDs in Actinobacteria From a Mexican Volcano Crater: Genomics and Bioremediation Potential

Journal of Basic Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

ABSTRACT Non‐steroidal anti‐inflammatory drugs (NSAIDs) are emerging contaminants that pose significant health and environmental risks due to their persistence, including presence in drinking water. Bioremediation, particularly through microorganisms such as actinobacteria, offers a sustainable approach mitigate these pollutants. Actinobacteria from poly‐extreme environments exhibit unique genetic metabolic adaptations, enabling resistance degradation of various contaminants. This study aimed evaluate the tolerance actinobacteria NSAIDs conduct genomic analysis selected strain. were isolated crater Chichonal volcano [Chiapas, Mexico), resulting 16 isolates. Among these, Micrococcus luteus P8SUE1, yunnanensis P9AGU1, Kocuria rhizophila P1AGU3 demonstrated diclofenac, ibuprofen, paracetamol at concentrations 1 ppm, 10 100 respectively. Whole‐genome sequencing M. P9AGU1 identified genes linked aromatic compounds adaptations extreme conditions, highlighting its potential for bioremediation applications.

Language: Английский

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Novel phytotoxic compounds from Streptomyces sp. Caat 7–52: a potential bioherbicide against Conyza canadensis

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Abstract BACKGROUND The rise of herbicide‐resistant weeds like Conyza canadensis L. poses a challenge to modern agriculture, driving the need for eco‐friendly alternatives. Microbial metabolites from actinobacteria species offer promising weed‐control solutions. This study aims screen and identify an isolate Brazil's Caatinga biome that produces phytotoxic characterize its compounds. RESULTS An isolate, named as Caat 7–52, was selected because significant effects against Lemna minor Phylogenetic analyses using six concatenated genes ( gyrB , recA rpoB trpB atpD 16S rRNA ) confirmed 7–52's close relationship Streptomyces musisoli TBRC 9950 T despite phenotypical differences. Bioassay‐directed isolation revealed 3‐hydroxybenzoic acid albocycline phytotoxins, with minimum inhibitory concentrations 50.00 3.12 μg mL −1 respectively. Albocycline analogues were also detected exhibited moderate phytotoxicity in . In addition, effectively inhibited seed germination C. concentration 6.25 marking first report albocycline's activity. Direct use fermented broth selectively dicot weeds, offering sustainable solvent‐free weed management strategy. CONCLUSION discovery sp. 7–52 metabolites, combined direct application broth, represents advancement control. bioherbicidal approach offers environmentally friendly alternative managing resistant supports broader microbial integrated pest programs. © 2025 Society Chemical Industry.

Language: Английский

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Direct Cloning and Heterologous Expression of the Dmxorosin Biosynthetic Gene Cluster from Streptomyces thermolilacinus SPC6, a Halotolerant Actinomycete Isolated from the Desert in China

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1492 - 1492

Published: Feb. 11, 2025

Streptomyces thermolilacinus SPC6 is a halotolerant strain isolated from the Linze Desert in China. It has very high growth rate and short life cycle compared to other Streptomycetes, including model organism coelicolor. The one strain–many compounds fermentation approach global natural products investigation revealed that exhibits impressive productivity of secondary metabolites. Genome mining uncovered 20 typical metabolic biosynthetic gene clusters (BGC), with BGC dmx identified as completely silent. Subsequently, this cryptic was successfully directly cloned heterologously expressed hosts, resulting discovery new lanthipeptide, dmxorosin. Notably, proposed pathway indicates its potential basis for synthetic biology lanthipeptide.

Language: Английский

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