A Screening Model for Probiotics Against Specific Metabolic Diseases Based on Caco-2 Monolayer Membrane DOI Creative Commons
Liu Yang, Peng Jiang, Shiya Zhu

et al.

Engineering, Journal Year: 2022, Volume and Issue: 25, P. 222 - 233

Published: May 6, 2022

Recent studies have revealed the potency of probiotics in alleviating metabolic diseases associated with intestinal barrier dysfunction. However, an efficient model for screening probiotic strains against specific has not been well developed. In present study, a Caco-2 cell monolayer membrane treated tumor necrosis factor (TNF-α) or alcohol was used to evaluate effect 139 Lactobacillus on function vitro. We then selected 11 different regulatory abilities gut determine their ovariectomy-induced osteoporosis chronic alcoholic liver injury vivo. Our results showed that Pearson coefficient between data and animal models were 0.82 −0.97 protection disease, respectively, suggesting reliability simulate vivo protective effects probiotics. This study established potential vitro approach based such as disease.

Language: Английский

Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis DOI Creative Commons
Chun‐Yuan Chen,

Shan‐Shan Rao,

Tao Yue

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(15)

Published: April 13, 2022

Osteonecrosis of the femoral head (ONFH) commonly occurs after glucocorticoid (GC) therapy. The gut microbiota (GM) participates in regulating host health, and its composition can be altered by GC. Here, this study demonstrates that cohousing with healthy mice or colonization GM from normal attenuates GC-induced ONFH. 16S rRNA gene sequencing shows rescues reduction Lactobacillus animalis. Oral supplementation L. animalis mitigates ONFH increasing angiogenesis, augmenting osteogenesis, reducing cell apoptosis. Extracellular vesicles (L. animalis-EVs) contain abundant functional proteins enter to exert proangiogenic, pro-osteogenic, antiapoptotic effects, while abundance is reduced exposure Our suggests involved protecting transferring bacterial EVs, loss EVs associated development

Language: Английский

Citations

117
Modulation of bone remodeling by the gut microbiota: a new therapy for osteoporosis DOI Creative Commons
Zhengtian Lyu, Yongfei Hu, Yuming Guo

et al.

Bone Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: June 9, 2023

The gut microbiota (GM) plays a crucial role in maintaining the overall health and well-being of host. Recent studies have demonstrated that GM may significantly influence bone metabolism degenerative skeletal diseases, such as osteoporosis (OP). Interventions targeting modification, including probiotics or antibiotics, been found to affect remodeling. This review provides comprehensive summary recent research on regulating remodeling seeks elucidate regulatory mechanism from various perspectives, interaction with immune system, interplay estrogen parathyroid hormone (PTH), impact metabolites, effect extracellular vesicles (EVs). Moreover, this explores potential therapeutic approach for OP. insights presented contribute development innovative GM-targeted therapies

Language: Английский

Citations

76
Diets intervene osteoporosis via gut-bone axis DOI Creative Commons
Yuan‐Wei Zhang,

Peiran Song,

Sicheng Wang

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Jan. 4, 2024

Osteoporosis is a systemic skeletal disease that seriously endangers the health of middle-aged and older adults. Recently, with continuous deepening research, an increasing number studies have revealed gut microbiota as potential target for osteoporosis, research concept gut-bone axis has gradually emerged. Additionally, intake dietary nutrients adoption patterns may affect microbiota, alterations in might also influence metabolic status host, thus adjusting bone metabolism. Based on axis, can participate modulation metabolism by altering abundance, diversity, composition microbiota. Herein, combined emerging literatures relevant studies, this review aimed to summarize impacts different components osteoporosis acting well underlying mechanisms proper recommendations.

Language: Английский

Citations

55
Unlocking the Potential of Probiotics: A Comprehensive Review on Research, Production, and Regulation of Probiotics DOI
Tales Fernando da Silva, Rafael de Assis Glória, Monique Ferrary Américo

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2024, Volume and Issue: 16(5), P. 1687 - 1723

Published: March 28, 2024

Language: Английский

Citations

22
Lactobacillus plantarum attenuates glucocorticoid-induced osteoporosis by altering the composition of rat gut microbiota and serum metabolic profile DOI Creative Commons
Siying Li, Xuebing Han, Naiyuan Liu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 9, 2024

Introduction Osteoporosis, one of the most common non-communicable human diseases worldwide, is prevalent disease adult skeleton. Glucocorticoid-induced osteoporosis(GIOP) foremost form secondary osteoporosis, extensively researched due to its prevalence.Probiotics constitute a primary bioactive component within numerous foods, offering promise as potential biological intervention for preventing and treating osteoporosis. This study aimed evaluate beneficial effects probiotic Lactobacillus plantarum on bone health underlying mechanisms in rat model glucocorticoid dexamethasone-induced using osteoporosis treatment drug alendronate reference. Methods We examined microstructure (Micro-CT HE staining) analyzed gut microbiome serum metabolome rats. Results discussion The results revealed that L. significantly restored parameters microstructure, with elevated density, increased number thickness trabeculae, decreased Tb.Sp. Gut microbiota sequencing showed microbial diversity ratio Firmicutes Bacteroidota decreased. Beneficial bacteria abundance was ( Lachnospiraceae _NK4A136_group, Ruminococcus , UCG_005 Romboutsia Christensenellaceae _R_7_group), harmful Desulfovibrionaceae ). According metabolomics, significant changes metabolites occurred different groups. These differential were predominantly enriched pathways Pentose Glucuronate Interconversions, well Propanoate Metabolism. Furthermore, levels Pyrazine gamma-Glutamylcysteine, which associated inhibition osteoclast formation promoting osteoblast formation. can protect rats from DEX-induced GIOP by mediating “gut microbial-bone axis” production metabolites. Therefore candidate GIOP.

Language: Английский

Citations

16
Crosstalk between the gut microbiota and postmenopausal osteoporosis: Mechanisms and applications DOI
Qin Xu, Dan Li, Jing Chen

et al.

International Immunopharmacology, Journal Year: 2022, Volume and Issue: 110, P. 108998 - 108998

Published: July 1, 2022

Language: Английский

Citations

47
Gut–X axis DOI Creative Commons
Lin Xu, Yu Zhang, Xueyan Li

et al.

iMeta, Journal Year: 2025, Volume and Issue: 4(1)

Published: Feb. 1, 2025

Abstract Recent advances in understanding the modulatory functions of gut and microbiota on human diseases facilitated our focused attention contribution to pathophysiological alterations many extraintestinal organs, including liver, heart, brain, lungs, kidneys, bone, skin, reproductive, endocrine systems. In this review, we applied “gut–X axis” concept describe linkages between other organs discussed latest findings related axis,” underlying mechanisms potential clinical intervention strategies.

Language: Английский

Citations

1
New insights into dairy management and the prevention and treatment of osteoporosis: The shift from single nutrient to dairy matrix effects—A review DOI
Kaili Wang,

Xu Zhao,

Sijia Yang

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(4)

Published: June 7, 2024

Abstract Dairy is recognized as a good source of calcium, which important for preventing osteoporosis. However, the relationship between milk and bone health more complex than just calcium supplementation. It unwise to focus solely on observing effects single nutrient. Lactose, proteins, vitamins in milk, well fatty acids, oligosaccharides, exosomes, all work together with enhance its bioavailability utilization efficiency through various mechanisms. We evaluate roles dairy nutrients active ingredients maintaining homeostasis from perspective matrix effects. Special attention given threshold effects, synergistic associations gut–bone axis. also summarize probiotic/prebiotic low‐fat/high‐fat lactose‐free fortified reduced risk osteoporosis discuss potential benefits controversies these products. Moreover, we examine role products increasing peak mass during adolescence reducing loss old age. provides theoretical reference use accurate prevention management related chronic diseases offers personalized dietary recommendations different populations.

Language: Английский

Citations

5
New Advances in Improving Bone Health Based on Specific Gut Microbiota DOI Creative Commons

Qihui Yan,

Liping Cai, Weiying Guo

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: July 4, 2022

The gut microbiota has been shown to play an important role in the pathogenesis of various diseases, including metabolic cardiovascular and cancer. Recent studies suggest that is also closely associated with bone metabolism. However, given high diversity microbiota, effects different taxa compositions on are poorly understood. Previous demonstrated mechanisms underlying mainly include its modulation nutrient absorption, intestinal permeability, metabolites (such as short-chain amino acids), immune responses, hormones or neurotransmitters 5-hydroxytryptamine). Several found external interventions, such dietary changes, improved health altered composition microbiota. This review summarises beneficial bacteria explores how dietary, natural, physical factors alter improve health, thereby providing potential new insight into prevention osteoporosis.

Language: Английский

Citations

19
Genus_Ruminococcus and order_Burkholderiales affect osteoporosis by regulating the microbiota-gut-bone axis DOI Creative Commons
Ning Li, Haiyang Wang,

Huan Pei

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 21, 2024

Background This study aimed to clarify the relationship between gut microbiota and osteoporosis combining Mendelian randomization (MR) analysis with animal experiments. Methods We conducted an on differential bacteria using open-access genome-wide association (GWAS) data microbe obtained from public databases. The was performed two-sample MR analysis, causal examined through inverse variance weighting (IVW), Egger, weighted median, mode methods. Bilateral oophorectomy employed replicate mouse model, which assessed by micro computed tomography (CT), pathological tests, bone transformation indexes. Additionally, 16S rDNA sequencing fecal samples, while SIgA indexes of IL-6, IL-1β, TNF-α inflammatory factors were in colon samples. Through immunofluorescence histopathology, expression levels tight junction proteins, such as claudin-1, ZO-1, occludin, assessed, conduct correlation related environmental performed. Results A positive observed g_Ruminococcus1 risk osteoporosis, O_Burkholderiales showed a negative osteoporosis. Furthermore, there no evidence heterogeneity or pleiotropy. successful replication model it found that abundance significantly reduced, g_Ruminococcus increased ovariectomized (OVX)-mice. intestinal level OVX mice decreased, increased, barrier damage occurred, content LPS serum increased. is strongly positively correlated formation factors, indicators, density, volume fraction, trabecular quantity, whereas negatively resorption shows strong factors. Conclusion may regulate development microbiota-gut-bone axis.

Language: Английский

Citations

4