Multi-omics integration identifies molecular markers and biological pathways for carcass and meat quality traits in Nellore cattle DOI Creative Commons
Gabriela Bonfá Frezarim, Lúcio Flávio Macêdo Mota, Larissa Fernanda Simielli Fonseca

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 26, 2025

Understanding the biological factors that influence carcass and meat quality traits in Nellore cattle requires a clear interpretation of molecular complexity its variations at different levels expression. Hence, this study aimed to elucidate control by integrating genome-wide association (GWAS), transcriptomic, proteomic data, focusing on identifying key genes pathways. GWAS analysis was performed using weighted single-step GBLUP with two iterations. RNA-Seq analyses were 24 muscle samples from animals divergent adjusted phenotypic values (12 for high 12 low), tenderness, ribeye area (REA), marbling, backfat thickness (BFT). The systematic effects contemporary groups age. Differential expression indicated associated promotion growth processes, such as FRZB, IGFBP5 SEMA6C, exhibited overexpression within group characterized higher tenderness inhibits cellular cycles (RTN4 RB1) downregulated. Proteins related heat shock, structural functions, metabolic regulation also affected group. For actin-binding proteins, microtubule-forming proteins downregulated, while involved fatty acid composition synthesis upregulated, transcripts CAND1, ACTN4, FGFR2, NCOR2 identified. BFT, neuronal genes, transcripts, actin cytoskeleton organization microtubule formation found. Key ubiquitination, energy metabolism, tissue remodeling These findings provide better understanding pathways cattle.

Language: Английский

Multi-omics integration identifies molecular markers and biological pathways for carcass and meat quality traits in Nellore cattle DOI Creative Commons
Gabriela Bonfá Frezarim, Lúcio Flávio Macêdo Mota, Larissa Fernanda Simielli Fonseca

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 26, 2025

Understanding the biological factors that influence carcass and meat quality traits in Nellore cattle requires a clear interpretation of molecular complexity its variations at different levels expression. Hence, this study aimed to elucidate control by integrating genome-wide association (GWAS), transcriptomic, proteomic data, focusing on identifying key genes pathways. GWAS analysis was performed using weighted single-step GBLUP with two iterations. RNA-Seq analyses were 24 muscle samples from animals divergent adjusted phenotypic values (12 for high 12 low), tenderness, ribeye area (REA), marbling, backfat thickness (BFT). The systematic effects contemporary groups age. Differential expression indicated associated promotion growth processes, such as FRZB, IGFBP5 SEMA6C, exhibited overexpression within group characterized higher tenderness inhibits cellular cycles (RTN4 RB1) downregulated. Proteins related heat shock, structural functions, metabolic regulation also affected group. For actin-binding proteins, microtubule-forming proteins downregulated, while involved fatty acid composition synthesis upregulated, transcripts CAND1, ACTN4, FGFR2, NCOR2 identified. BFT, neuronal genes, transcripts, actin cytoskeleton organization microtubule formation found. Key ubiquitination, energy metabolism, tissue remodeling These findings provide better understanding pathways cattle.

Language: Английский

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