Scrutinising an inscrutable bark-nesting ant: Exploring cryptic diversity in the Rhopalomastix javana (Hymenoptera: Formicidae) complex using DNA barcodes, genome-wide MIG-seq and geometric morphometrics DOI Creative Commons
Wendy Y. Wang, Aiki Yamada

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e16416 - e16416

Published: Nov. 16, 2023

Overlooking cryptic species diversity has grave implications on assessments of climate change impacts biodiversity, ecosystems and organismal populations. Discriminating between long been challenging even for seasoned taxonomists, as interspecies morphological differences are often indiscernible by visual observation. Multi-disciplinary methods involving genetic analyses in conjunction with quantitative data, should therefore be used to investigate boundaries species. We adopted an integrated approach combining mitochondrial COI barcodes, a genome-wide dataset obtained via multiplexed inter-simple sequence repeats (ISSRs) genotyping sequencing (MIG-seq), geometric morphometrics divergences the inscrutable Rhopalomastix javana complex. Objective clustering suggested five putative molecular units divergent from each other thresholds within 4.2-10.6% uncorrected pairwise distance. Phylogenetic based concatenated MIG-seq data also recovered strongly supported monophyly major lineages agreement clusters. Co-ancestry using fineRADstructure resolved variable patterns admixture linked geography, potential drift some Geometric morphometric specimen images further detected statistically significant at least one three anatomical aspects (Head, Meso, Profile) all pairs Head shape (full-face view) was determined most informative character diagnosis, relatively high classification accuracy. Thin-plate spline deformation grids highlighted areas variation deeper taxonomic scrutiny. The presence multiple distinct existing near-sympatry firmly demonstrates that R. comprises more than closely-related species, but exact difficult ascertain. Differences elevation its associated abiotic effects ant adaptations reproductive phenology may contribute restricting gene flow maintaining sympatric populations assess advantages limitations tool. Despite drawbacks, our combined helped draw important insights javana, identified gaps knowledge await address. Results this study will inform prime future in-depth investigation complex, including formal descriptions establishment

Language: Английский

Unveiling Cryptic Diversity in Hylomys: A Commentary on Recent Taxonomic Revisions DOI Open Access
Kai He, Ying Zeng, Xing Chen

et al.

Integrative Zoology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

The genus Hylomys now comprises seven species instead of two; the in China should be classified as peguensis.

Language: Английский

Citations

0
Mitogenomic analysis of Thai Sunda pangolins reveals regional phylogeography and informs conservation management DOI Creative Commons

Nattapong Banterng,

Kyle M. Ewart, Frankie Thomas Sitam

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 23, 2025

Abstract Pangolins are considered the most trafficked mammals in world with all eight species listed on CITES Appendix I. Despite this pervasive threat to their survival, there remains a limited understanding of genetic diversity and connectivity among populations Asian pangolin species, hampering effective conservation management. We analysed mitogenome sequences Sunda ( Manis javanica ) from across Southeast Asia continental distribution, as well Borneo. Phylogenetic reconstruction revealed six lineages, clear separation north south Kangar-Pattani biogeographic line southern Thailand, revealing differentiation between Sundaland Indochinese lineages. Further divergence an east–west divide was observed central northern extending northwards towards China. Our results provide new insights into evolutionary relationships Asia, building other recent research field helping establish species’ baseline phylogeography. These inferences will aid planning support traceability illegal trade.

Language: Английский

Citations

0
Lost in synonymy: Integrative species delimitation reveals two unrecognized species of Southern Asian tree squirrels (Rodentia: Sciuridae: Callosciurinae) DOI Creative Commons
Arlo Hinckley, Jesús E. Maldonado, Noriko Tamura

et al.

Vertebrate Zoology, Journal Year: 2024, Volume and Issue: 74, P. 683 - 707

Published: Oct. 28, 2024

Abstract We present a comprehensive integrative taxonomic review of Callosciurus caniceps and Tamiops mcclellandii as they are currently defined. This combines published molecular evidence, craniodental morphometrics, pelage bacular variation, evaluations potential hybrid zones using museum specimens citizen science photographs, and, for C. , bioacoustic evidence. Our findings lead to the recognition two species that had been lost in synonymy highlight future perspectives on delimitation Sciuridae. By comparing phenotypic differentiation across climatic vegetation transitions contextualizing our results with evolutionary history study systems, we provide insights into distribution, ecogeographical patterns, speciation drivers Southeast Asian vertebrates.

Language: Английский

Citations

2
Speciation on the Roof of the World: Parallel Fast Evolution of Cryptic Mole Vole Species in the Pamir-Alay—Tien Shan Region DOI Creative Commons
А. С. Богданов, Valentina Tambovtseva, Sergey Matveevsky

et al.

Life, Journal Year: 2023, Volume and Issue: 13(8), P. 1751 - 1751

Published: Aug. 16, 2023

Speciation is not always accompanied by morphological changes; numerous cryptic closely related species were revealed using genetic methods. In natural populations of Ellobius tancrei (2n = 54-30) and E. alaicus 52-48) the Pamir-Alay Tien Shan, chromosomal variability due to Robertsonian translocations has been revealed. Here, comprehensive analysis (karyological analyses as well sequencing mitochondrial genes, cytb COI, nuclear XIST IRBP) samples from Inner Alay Valley, Pamir-Alay, we demonstrated fast independent diversification these species. We described an incompletely consistent polymorphism markers, which arose presumably because habitat fragmentation in highlands, rapid karyotype changes, hybridization different intraspecific varieties The most intriguing results are a low level distances calculated genes between some phylogenetic lines alaicus, significant species-specific chromosome both rearrangements what clearly define specificity provide further diversification. "mosaicism" inconsistency patterns evidence speciation mammals.

Language: Английский

Citations

4
Mitogenome-based genetic management of captive Great Hornbill in Thailand: Implications for reintroduction DOI Creative Commons

Pimpisa Jansamut,

George A. Gale, Manakorn Sukmak

et al.

Global Ecology and Conservation, Journal Year: 2024, Volume and Issue: 51, P. e02932 - e02932

Published: April 8, 2024

The Great Hornbill (Buceros bicornis) is globally vulnerable due to high rates of deforestation and hunting are therefore a target for restoration. Captive breeding programs in Thailand maintain many Hornbills, however active genetic management captive hornbills was not incorporated into the species recovery plan. With reintroductions planned northern immediate future, lack profiles phylogenetic relationships among potential founders might hamper population efforts. We assessed mitochondrial genome (mitogenome) diversity, structure, evolutionary history 97 Hornbills across five zoos Thailand. Among samples, we found 21 haplotypes with haplotype nucleotide diversity h = 0.81336 ± 0.021 pi 0.00108 0.00040, respectively. maintenance mitogenome no differentiation between populations (FST 0.00000 - 0.09723, p > 0.05) be result frequent founder exchange facilities. Phylogenetic analysis revealed existence southern clade BBITH13, indicating genetically distinct population. To protect these lineages adaptability that have been reintroduced, facilities contain unique should given priority plans. Our data support value hornbill highlight significance incorporating strategies plan Hornbills.

Language: Английский

Citations

1
Surrounded by Kindred: Spermophilus major Hybridization with Other Spermophilus Species in Space and Time DOI Creative Commons
Andrey Tukhbatullin, Oleg A. Ermakov,

Svetlana Yu. Kapustina

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(6), P. 880 - 880

Published: June 17, 2023

Among the numerous described cases of hybridization in mammals, most intriguing are (a) introgressive deeply affecting evolutionary history species, and (b) models involving not a pair species but multi-species complex. Therefore, russet ground squirrel Spermophilus major, whose range has repeatedly changed due to climatic fluctuations now borders ranges four related is great interest. The main aims this study were determine direction intensity gene introgression, spatial depth infiltration extraneous genes into S. major range, refine hypothesis hybridogenic replacement mitochondrial genomes studied group. Using phylogenetic analysis variability (CR, cytb) nuclear (SmcY, BGN, PRKCI, c-myc, i6p53) markers, we determined contribution neighboring genome. We showed that 36% individuals had alleles. All peripheral contact with contributed towards its genetic variability. also proposed for sequence localization serial events. Our assessment genome implications introgression highlights importance implementing conservation measures protect species.

Language: Английский

Citations

2
Scrutinising an inscrutable bark-nesting ant: Exploring cryptic diversity in the Rhopalomastix javana (Hymenoptera: Formicidae) complex using DNA barcodes, genome-wide MIG-seq and geometric morphometrics DOI Creative Commons
Wendy Y. Wang, Aiki Yamada

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e16416 - e16416

Published: Nov. 16, 2023

Overlooking cryptic species diversity has grave implications on assessments of climate change impacts biodiversity, ecosystems and organismal populations. Discriminating between long been challenging even for seasoned taxonomists, as interspecies morphological differences are often indiscernible by visual observation. Multi-disciplinary methods involving genetic analyses in conjunction with quantitative data, should therefore be used to investigate boundaries species. We adopted an integrated approach combining mitochondrial COI barcodes, a genome-wide dataset obtained via multiplexed inter-simple sequence repeats (ISSRs) genotyping sequencing (MIG-seq), geometric morphometrics divergences the inscrutable Rhopalomastix javana complex. Objective clustering suggested five putative molecular units divergent from each other thresholds within 4.2-10.6% uncorrected pairwise distance. Phylogenetic based concatenated MIG-seq data also recovered strongly supported monophyly major lineages agreement clusters. Co-ancestry using fineRADstructure resolved variable patterns admixture linked geography, potential drift some Geometric morphometric specimen images further detected statistically significant at least one three anatomical aspects (Head, Meso, Profile) all pairs Head shape (full-face view) was determined most informative character diagnosis, relatively high classification accuracy. Thin-plate spline deformation grids highlighted areas variation deeper taxonomic scrutiny. The presence multiple distinct existing near-sympatry firmly demonstrates that R. comprises more than closely-related species, but exact difficult ascertain. Differences elevation its associated abiotic effects ant adaptations reproductive phenology may contribute restricting gene flow maintaining sympatric populations assess advantages limitations tool. Despite drawbacks, our combined helped draw important insights javana, identified gaps knowledge await address. Results this study will inform prime future in-depth investigation complex, including formal descriptions establishment

Language: Английский

Citations

1