The utility of oral brush cytology in the early detection of oral cancer and oral potentially malignant disorders: A systematic review

Journal of Oral Pathology and Medicine, Journal Year: 2017, Volume and Issue: 47(2), P. 104 - 116

Published: Dec. 7, 2017

This systematic review aimed to analyze the published evidence for use of oral brush cytology early detection cancer and potentially malignant disorders (OPMDs).Literature was systematically searched through several databases: MEDLINE, EMBASE, PubMed, SCOPUS, Cochrane Library, Web Science. Additional performed cross-checks on bibliographies selected articles. The inclusion criteria involved studies assessing utility human tissues its applications in diagnosis, screening, or surveillance OPMDs.The search strategy resulted 343 abstracts full-text articles, which 36 met criteria. year publication ranged from 1994 2017, a total 4302 samples OPMDs, squamous cell carcinoma, healthy controls have been investigated. Baby toothbrush, cytobrush, OralCDx® , Orcellex® are brushes that were used obtain transepithelial mucosal conventional liquid-based evaluation.Findings this study indicate meaningful evidence-based recommendations implementation minimally invasive technique be utilized as an adjunctive tool screening OPMDs complicated reported literature. There is need well-designed clinical assess accuracy utilizing validated cytological assessment diagnosis prediction OPMDs.

Language: Английский

---

LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer

Journal of Experimental & Clinical Cancer Research, Journal Year: 2019, Volume and Issue: 38(1)

Published: June 11, 2019

Colorectal cancer (CRC) is the third most frequent and second leading cause of cancer-related death worldwide. Increasing evidence indicates that deregulation long noncoding RNAs (lncRNAs) contributes to tumor initiation progression; however, little known about biological role susceptibility candidate 9 (CASC9) in CRC. Novel lncRNAs potentially involved CRC tumorigenesis were identified from datasets downloaded The Cancer LncRNome Atlas Noncoding Cancer. cell lines HCT-116, HCT-116 p53−/−, SW620, SW480, HT-29, LoVo, LS-174T, RKO used. Colony-formation, MTS, cell-cycle, apoptosis, in-vivo assays used determine CASC9 growth vitro vivo. Potential interaction between cleavage polyadenylation specificity factor subunit 3 (CPSF3) was evaluated using RNA immunoprecipitation RNA-protein pull-down assays. RNA-sequencing performed analyze gene expression following knockdown. RT-qPCR, western blotting, mRNA decay study mechanisms involved. frequently upregulated CRC, which correlated with advanced TNM stage, higher levels associated poor patient outcomes. Knockdown inhibited promoted apoptosis cells, whereas ectopic We demonstrated CPSF3 a CASC9-interacting protein, knockdown mimicked effects cells. Furthermore, we found exerts its oncogenic activity by modulating TGFβ2 stability upregulating TERT, resulting an increase phosphorylated SMAD3 activation TGF-β signaling, enhanced TERT complex function Finally, significantly tissues as compared adjacent or non-adjacent normal colon tissues, CASC9, CPSF3, human positively correlated. promising prognostic predictor for patients CASC9-CPSF3-TGFβ2 axis potential therapeutic target treatment.

Language: Английский

---

circHECTD1 facilitates glutaminolysis to promote gastric cancer progression by targeting miR-1256 and activating β-catenin/c-Myc signaling

Cell Death and Disease, Journal Year: 2019, Volume and Issue: 10(8)

Published: Aug. 1, 2019

Abstract Circular RNAs (circRNAs) have emerged as crucial regulators of human cancers. Glutaminolysis supplies cancer cells with adequate nitrogen and carbon to replenish the tricarboxylic acid cycle, contributing survival progression tumor cells. However, association between circRNAs glutaminolysis remains unclear. In this study, we showed that circHECTD1 expression was markedly upregulated in gastric (GC) associated lymph node metastasis American Joint Committee on Cancer stage. The level found be an independent prognostic factor for GC patients. knockdown inhibited cell glutaminolysis, proliferation, migration, invasion, whereas overexpression promoted progression. Dual-luciferase RNA immunoprecipitation assays demonstrated miR-1256 a direct downstream target circHECTD1. targeted subsequently increased USP5. circHECTD1/miR-1256/USP5 axis exerted its tumor-promoting effects by activating β-catenin/c-Myc signaling pathway. vivo mouse models further verified oncogenic roles GC. Our results revealed is glutaminolysis-associated circRNA promotes could thus used therapeutic

Language: Английский

---

Up-regulated lncRNA XIST contributes to progression of cervical cancer via regulating miR-140-5p and ORC1

Cancer Cell International, Journal Year: 2019, Volume and Issue: 19(1)

Published: Feb. 28, 2019

The study purpose was to make investigation into the influence of XIST on cervical cancer progression and what's more its potential mechanism.The data sets (lncRNA, miRNA, mRNA) obtained from TCGA were analyzed with "mixOmics" R package. Then, expression XIST, miR-140-5p, ORC1 detected using qRT-PCR western blot in both tissues cell lines (Hela C33A) verify bioinformatics analyses results. CCK-8 assay, 5-ethynyl-2'-deoxyuridine (EdU) assays, cycle assay apoptosis practiced. Besides, immunohistochemistry staining operated for detection Ki-67, E-cadherin vimentin apoptosis-related proteins (c-caspase3, Bcl-2, total PARP cleaved PARP) verified through blot. And vivo experiments implemented.MiR-140-5p down-regulated but up-regulated lines. Knocking down or memorably suppressed proliferation, blocked cycle, decreased Bcl-2 while increased rate c-caspase3 HeLa C33A cells. results showed knocking improved levels Ki-67 expression. overexpression miR-140-5p also could inhibit reverse cells.Our indicated effects XIST/miR-140-5p/ORC1 axis which will shed new light epigenetic diagnostics therapeutics cancer.

Language: Английский

---

N6-Methyladenosine METTL3 Modulates the Proliferation and Apoptosis of Lens Epithelial Cells in Diabetic Cataract

Molecular Therapy — Nucleic Acids, Journal Year: 2020, Volume and Issue: 20, P. 111 - 116

Published: Feb. 11, 2020

N6-methyladenosine (m6A) is the most prevalent eukaryotic messenger RNA modification. Diabetic cataract (DC) caused by high glucose (HG) in diabetes mellitus. However, regulatory mechanism of m6A DC pathogenesis poorly understood. In present research, we performed m6A-RNA immunoprecipitation sequencing (MeRIP-Seq) analysis and detected modification profile HG- or normal (NG)-induced human lens epithelial cells (HLECs). Results revealed that methyltransferase-like 3 (METTL3) was upregulated tissue specimens HG-induced HLECs. Besides, total level higher Functionally, METTL3 knockdown promoted proliferation repressed apoptosis HLECs induced HG. MeRIP-Seq ICAM-1 might act as target METTL3. Mechanistically, targets 3′ UTR to stabilize mRNA stability. conclusion, this research identified regulation HLECs, providing a potential insight for DC.

Language: Английский

---

ALDH1: A potential therapeutic target for cancer stem cells in solid tumors

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: Oct. 28, 2022

Solid tumors can be divided into benign solid and malignant in the academic community, among which are called cancers. Cancer is second leading cause of death world, global incidence cancer increasing yearly New patients China always first. After concept stem cells was introduced tumor CSC markers represented by ALDH1 have been widely studied due to their strong cell characteristics potential driving force metastasis. In research results past five years, it has found that highly expressed various cancers such as breast cancer, lung colorectal liver gastric cervical esophageal ovarian head,and neck cancer. activate transform pathways (such USP28/MYC signaling pathway, ALDH1A1/HIF-1α/VEGF axis, wnt/β-catenin pathway), well change intracellular pH value promote formation maintenance, resulting drug resistance tumors. By targeting inhibiting cells, enhance sensitivity drugs inhibit proliferation, differentiation, metastasis some extent. This review discusses relationship pathway with It proposes may serve a diagnosis therapeutic target for CSC, providing new insights strategies reliable treatment.

Language: Английский

---

HDAC2 depletion promotes osteosarcoma’s stemness both in vitro and in vivo: a study on a putative new target for CSCs directed therapy

Journal of Experimental & Clinical Cancer Research, Journal Year: 2018, Volume and Issue: 37(1)

Published: Dec. 1, 2018

Cancer stem cells (CSCs) play a key role in cancer initiation, progression and chemoresistance. Epigenetic alterations have been identified as prominent factors that contribute to the CSCs phenotype. Here, we investigated effects of HDAC inhibitor valproic acid (VPA) demethylating agent, 5'azacytidine (DAC) on phenotype MG63 Saos2 osteosarcoma cell lines. were treated with DAC VPA, alone combination. Untreated examined for stemness by cytometry real-time PCR. Sarcospheres colonies formation also evaluated. Moreover, histone modification methylation tested flow cytomery western blotting. HDAC2 depleted their ability generate tumors NOD/SCID IL2R-gamma-0 (NSG) mice. expression human tissues was We found VPA induce an increased markers including CD133, OCT4, SOX2 NANOG, sarcospheres efficiency. Interestingly, showed treatment decreased repressive markers, while active ones. These modifications associated increase acetylation histones H3, decrease DNA global methylation, DNMT3a. Furthermore, silenced-MG63 acquired phenotype, promoted vivo tumorigenesis. In tissues, strongly expressed nucleus. Collectively, our results suggest expansion CSCs, report first time is factor regulating both growth. conclusion, potential therapeutic target treatment.

Language: Английский

---

Circular RNA circSCAF11 Accelerates the Glioma Tumorigenesis through the miR-421/SP1/VEGFA Axis

Molecular Therapy — Nucleic Acids, Journal Year: 2019, Volume and Issue: 17, P. 669 - 677

Published: July 5, 2019

Circular RNAs (circRNAs) are a novel category of non-coding RNAs, and they have been identified to participate in glioma tumorigenesis. Here we investigated the functions circRNA circSCAF11 genesis, unveiled its molecular mechanism pathophysiological process. Expression levels circSCAF11, miR-421, SP1 mRNA were measured using RT-PCR. Proteins western blotting. The tumor phenotypes cells detected flow cytometry Cell Counting Kit-8 (CCK-8), transwell, xenograft mouse assays. combination within was validated luciferase reporter assay or RNA pull-down assay. binding transcription factor with vascular endothelial cell growth A (VEGFA) promoter inspected chromatin immunoprecipitation (ChIP). expression found be significantly upregulated tissue specimens lines. ectopic overexpression closely correlated poor clinical outcome patients. Functionally, knockdown inhibited proliferation, invasion, induced G0/G1 phase arrest. Mechanically, positively regulated through sponging miR-421. Moreover, activated VEGFA, constructing circSCAF11/miR-421/SP1/VEGFA axis genesis. findings this research illustrate that accelerates tumorigenesis miR-421/SP1/VEGFA axis, providing potential target for treatment.

Language: Английский

---

SNHG20: A vital lncRNA in multiple human cancers

Journal of Cellular Physiology, Journal Year: 2019, Volume and Issue: 234(9), P. 14519 - 14525

Published: Jan. 15, 2019

Long noncoding RNAs (lncRNAs) act as an initial factor and promoter in different tumors a kind of ncRNAs. The length them is >200 nucleotides opposite small Increasing researches have proved that dysregulation lncRNA has been implicated tumorigenesis. Small nucleolar RNA host gene 20 (SNHG20), member lncRNAs, expresses frequently cancer types, such hepatocellular carcinoma, ovarian cancer, colorectal bladder contributing to development progression by transcriptional or posttranscriptional modifications. Not only does this review show the recent published literature concerning biological functions but also demonstrates molecular mechanisms SNHG20 among above multiple malignancies others.

Language: Английский

---

Noncoding RNAs: the shot callers in tumor immune escape

Signal Transduction and Targeted Therapy, Journal Year: 2020, Volume and Issue: 5(1)

Published: June 19, 2020

Abstract Immunotherapy, designed to exploit the functions of host immune system against tumors, has shown considerable potential several malignancies. However, utility immunotherapy is heavily limited due low response rate and various side effects in clinical setting. Immune escape tumor cells may be a critical reason for such rates. Noncoding RNAs (ncRNAs) have been identified as key regulatory factors tumors system. Consequently, ncRNAs show promise targets improve efficacy tumors. relationship between (TIE) not yet comprehensively summarized. In this review, we provide detailed account current knowledge on associated with TIE their roles growth survival mechanisms. This review bridges gap broadens our understanding relationship, providing new insights strategies rates by specifically targeting involved TIE.

Language: Английский

---