Circular RNA hsa_circ_0064559 affects tumor cell growth and progression of colorectal cancer DOI Creative Commons

Yanan Zhen,

Guodong Sun,

Cunbao Chen

et al.

World Journal of Surgical Oncology, Journal Year: 2023, Volume and Issue: 21(1)

Published: June 7, 2023

Colorectal cancer (CRC) is the second leading cause of cancer-related deaths globally. It essential to identify new CRC-associated therapeutic targets and diagnostic biomarkers. Previous studies have demonstrated that a series circular RNAs (circRNAs) play crucial role in CRC pathogenesis. This study assessed potential hsa_circ_0064559 tumor cell growth progression CRC.Six pairs matched normal colorectal tissue samples were sequenced using Affymetrix Clariom D array. Using RNA interference, expression thirteen circRNAs was knocked down cells. The proliferation lines (RKO SW620 cells) detected 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Apoptosis cycle determined by flow-cytometric analysis. An vivo uses nude mice establish mouse model. differentially expressed genes analyzed primeview human GeneChip array verified polymerase chain reaction.Affymetrix analysis revealed upregulated CRC. decreased, while proportion apoptotic G1 phase cells higher after knockdown. In xenograft model volume weight reduced array, we found six (STAT1, ATF2, TNFRSF10B, TGFBR2, BAX, SQSTM1) two downregulated (SLC4A7 CD274) related apoptosis knockdown.The knockdown could inhibit proliferation, promote vitro, development tumors vivo. mechanism may be activating wide range signaling pathways. biomarker for early diagnosis or prognosis novel drug target therapy.

Language: Английский

Advanced strategies of targeting circular RNAs as therapeutic approaches in colorectal cancer drug resistance DOI Creative Commons
Bashdar Mahmud Hussen, Snur Rasool Abdullah,

Abdulqahar Azizkhan Mohammed

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 260, P. 155402 - 155402

Published: June 14, 2024

Colorectal cancer (CRC) stands second in terms of mortality and third among the highest prevalent kinds globally. CRC prevalence is rising moderately poorly developed regions greater economically advanced regions. Despite breakthroughs targeted therapy, resistance to chemotherapeutics remains a significant challenge long-term management CRC. Circular RNAs (circRNAs) have been involved growing therapy resistance, particularly CRC, according an increasing number studies recent years. CircRNAs are one novel subclasses non-coding RNAs, previously thought as viroid. According studies, circRNAs recommended biological markers for therapeutic targets diagnostic prognostic purposes. That notable given that expression has linked hallmarks since they responsible drug patients; thereby, chemotherapy failure. Moreover, knowledge concerning relatively unclear despite using all these techniques. Here, this study, we will go over most published work highlight critical roles development main strategies overcome improve clinical outcomes.

Language: Английский

Citations

3
Circ_0082182 upregulates the NFIB level via sponging miR-326 to promote oxaliplatin resistance and malignant progression of colorectal cancer cells DOI
Zhifeng Wang, Jingmei Liu, Tao Yang

et al.

Molecular and Cellular Biochemistry, Journal Year: 2022, Volume and Issue: 478(5), P. 1045 - 1057

Published: Oct. 11, 2022

Language: Английский

Citations

12
Role of circular RNA and its delivery strategies to cancer – An overview DOI
Satya Siva Kishan Yalamarty, Nina Filipczak, Muhammad Muzamil Khan

et al.

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 356, P. 306 - 315

Published: March 9, 2023

Language: Английский

Citations

7
Exosomal circCOL1A2 from cancer cells accelerates colorectal cancer progression via regulating miR-665/LASP1 signal axis DOI

Miao Zhi,

Xiaomeng Zhao, Xiang Liu

et al.

European Journal of Pharmacology, Journal Year: 2023, Volume and Issue: 950, P. 175722 - 175722

Published: April 12, 2023

Language: Английский

Citations

7
Circular RNA hsa_circ_0064559 affects tumor cell growth and progression of colorectal cancer DOI Creative Commons

Yanan Zhen,

Guodong Sun,

Cunbao Chen

et al.

World Journal of Surgical Oncology, Journal Year: 2023, Volume and Issue: 21(1)

Published: June 7, 2023

Colorectal cancer (CRC) is the second leading cause of cancer-related deaths globally. It essential to identify new CRC-associated therapeutic targets and diagnostic biomarkers. Previous studies have demonstrated that a series circular RNAs (circRNAs) play crucial role in CRC pathogenesis. This study assessed potential hsa_circ_0064559 tumor cell growth progression CRC.Six pairs matched normal colorectal tissue samples were sequenced using Affymetrix Clariom D array. Using RNA interference, expression thirteen circRNAs was knocked down cells. The proliferation lines (RKO SW620 cells) detected 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Apoptosis cycle determined by flow-cytometric analysis. An vivo uses nude mice establish mouse model. differentially expressed genes analyzed primeview human GeneChip array verified polymerase chain reaction.Affymetrix analysis revealed upregulated CRC. decreased, while proportion apoptotic G1 phase cells higher after knockdown. In xenograft model volume weight reduced array, we found six (STAT1, ATF2, TNFRSF10B, TGFBR2, BAX, SQSTM1) two downregulated (SLC4A7 CD274) related apoptosis knockdown.The knockdown could inhibit proliferation, promote vitro, development tumors vivo. mechanism may be activating wide range signaling pathways. biomarker for early diagnosis or prognosis novel drug target therapy.

Language: Английский

Citations

7