Exploring the role of Disulfidptosis in glioma progression: insights into tumor heterogeneity and therapeutic potential through single-cell RNA sequencing DOI Creative Commons
Xiaoli Fan, Maojun Chen

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 23, 2024

Gliomas, particularly glioblastoma (GBM), are the most common and aggressive primary brain tumors in adults, characterized by high malignancy frequent recurrence. Despite standard treatments, including surgery, radiotherapy, chemotherapy, prognosis for GBM remains poor, with a median survival of less than 15 months five-year rate below 10%. Tumor heterogeneity resistance to treatment create significant challenges controlling glioma progression. Therefore, there is an urgent need new therapeutic targets strategies. This study investigates role Disulfidptosis, recently discovered form programmed cell death, gliomas. Unlike apoptosis necrosis, Disulfidptosis driven abnormal accumulation intracellular disulfide bonds, leading protein misfolding cytoskeletal collapse, cancer cells metabolic dysregulation. We aim explore how respond identify potential analyzing gliomas at single-cell level using RNA sequencing (scRNA-seq). scRNA-seq data from patients were analyzed uncover differences ferroptosis-related pathways, iron metabolism lipid peroxidation. Cellular subpopulations within profiled assess their sensitivity underlying mechanisms. Survival analysis was conducted evaluate clinical relevance Disulfidptosis-related gene expression. Multiple exhibit varying sensitivities influenced properties. Dysregulated antioxidant mechanisms identified as key factors impacting sensitivity. Glioma microenvironment signaling pathways also play regulating Disulfidptosis. These findings suggest that activating may provide novel strategies overcome offers insights into progression highlights its target. By leveraging data, research uncovers tumor identifies specific populations resistant pave way personalized improve outcomes patients.

Language: Английский

Elucidating stearoyl metabolism and NCOA4-mediated ferroptosis in gastric cancer liver metastasis through multi-omics single-cell integrative mendelian analysis: advancing personalized immunotherapy strategies DOI Creative Commons

Zhongqiu Yang,

Yuquan Chen, Yaping Miao

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 15, 2025

The metabolism of stearoyl-GPE plays a key role in the liver metastasis gastric cancer. This investigation delves into mechanisms underlying intricate tumor microenvironment (TME) heterogeneity triggered by stearoyl cancer with (LMGC), offering novel perspectives for LMGC. Utilizing Mendelian randomization, we determined that significantly contributes to progression (GC). Following this, bulk transcriptome analyses and single-cell multiomics techniques investigate roles metabolism-related genes, particularly NCOA4, regulating LMGC TME. Our analysis highlights crucial modulating complex LMGC, impacting monocyte cells. Through sequencing spatial transcriptomics, have identified metabolic genes specific within cell population, including NCOA4. Regarding relationship between ferroptosis, metabolism, findings, it is plausible pathways intersect involved ferroptosis. Ferroptosis, characterized iron-dependent lipid peroxidation, represents regulated form death. activity Stearoyl-CoA desaturase (SCD), critical enzyme has been associated modulation composition susceptibility Furthermore, integral cellular processes related oxidative stress both which are significant factors context study enhances understanding ferroptosis promoting its regulation heterogeneity. In addition, this deeper dynamics provides basis development better interventions combat metastasis.

Language: Английский

Citations

1
Integrating multi-omics and experimental techniques to decode ubiquitinated protein modifications in hepatocellular carcinoma DOI Creative Commons
Haikun Yang, Yuan Chen, Zheng Zhou

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 11, 2025

Background Ubiquitination, a critical post-translational modification, plays pivotal role in regulating protein stability and activity, influencing various aspects of cancer development, including metabolic reprogramming, immune evasion, tumor progression. However, the specific ubiquitination hepatocellular carcinoma (HCC), particularly relation to microenvironment (TME), remains poorly understood. This study aims systematically explore shaping TME HCC, with focus on its impact progression modulation. Methods We performed bioinformatics analysis by integrating multiple publicly available HCC datasets assess status across cell types TME, plasma cells, fibroblasts, endothelial epithelial-mesenchymal transition (EMT) cells. Ubiquitination scores were calculated categorize these types, survival data, along spatial transcriptomics, employed evaluate how different levels influence In vitro experiments, such as transwell, CCK8, wound healing assays, used further investigate key gene UBE2C phenotypes. Results Our revealed that ubiquitination-related genes are significantly upregulated tissues, high expression correlating poor prognosis patients. Pathway showed enriched processes cycle regulation, DNA repair, p53 signaling. These pathways contribute promoting proliferation, facilitating matrix remodeling, enhancing angiogenesis. Notably, UBE2C, enzyme, appears play potentially inhibiting anti-tumor responses reducing system’s ability recognize eliminate Furthermore, experimental data confirmed overexpression promotes invasion, metastasis, supporting remodeling. Conclusion reveals multifaceted regulatory roles HCC. not only supports proliferation anti-apoptotic functions within cells but also modulating activity stromal Among all genes, emerges potential prognostic biomarker therapeutic target offering new directions for precision treatment future.

Language: Английский

Citations

0
ATP6AP1 drives pyroptosis-mediated immune evasion in hepatocellular carcinoma: a machine learning-guided therapeutic target DOI Creative Commons
Lei Tang, Xiyue Wang,

Zhengzheng Xia

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 25, 2025

Language: Английский

Citations

0
Single-cell transcriptomics reveals heterogeneity and prognostic markers of myeloid precursor cells in acute myeloid leukemia DOI Creative Commons
Guanghua He, Lai Jiang, Xuancheng Zhou

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 16, 2024

Acute myeloid leukemia (AML) is a hematologic tumor with poor prognosis and significant clinical heterogeneity. By integrating transcriptomic data, single-cell RNA sequencing data independently collected this study aims to identify key genes in AML establish prognostic assessment model improve the accuracy of prediction.

Language: Английский

Citations

2
Exploring copper metabolism-induced cell death in gastric cancer: a single-cell RNA sequencing study and prognostic model development DOI Creative Commons
Yi Chen,

Yunmei Liao,

Lang Huang

et al.

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 27, 2024

Language: Английский

Citations

0
Integrating multi-omics techniques and in vitro experiments reveals that GLRX3 regulates the immune microenvironment and promotes hepatocellular carcinoma cell proliferation and invasion through iron metabolism pathways DOI Creative Commons
Yang Li, Yuan Chen, Yang Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 25, 2024

Hepatocellular carcinoma (HCC) is a common malignancy worldwide, and its development closely related to abnormalities in iron metabolism. This study aims systematically analyze changes metabolism the tumor microenvironment of HCC using single-cell sequencing technology, investigate potential mechanisms by which regulation affects survival liver cancer patients.

Language: Английский

Citations

0
Innovative strategies to optimise colorectal cancer immunotherapy through molecular mechanism insights DOI Creative Commons

Quanjun Lin,

Zhiqiang Wang, Jue Wang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 9, 2024

Colorectal cancer (CRC) is a leading cause of cancer-related deaths globally. The heterogeneity the tumor microenvironment significantly influences patient prognosis, while diversity cells shapes its unique characteristics. A comprehensive analysis molecular profile crucial for identifying novel targets drug sensitivity and uncovering pathophysiological mechanisms underlying CRC.

Language: Английский

Citations

0
Exploring the role of Disulfidptosis in glioma progression: insights into tumor heterogeneity and therapeutic potential through single-cell RNA sequencing DOI Creative Commons
Xiaoli Fan, Maojun Chen

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 23, 2024

Gliomas, particularly glioblastoma (GBM), are the most common and aggressive primary brain tumors in adults, characterized by high malignancy frequent recurrence. Despite standard treatments, including surgery, radiotherapy, chemotherapy, prognosis for GBM remains poor, with a median survival of less than 15 months five-year rate below 10%. Tumor heterogeneity resistance to treatment create significant challenges controlling glioma progression. Therefore, there is an urgent need new therapeutic targets strategies. This study investigates role Disulfidptosis, recently discovered form programmed cell death, gliomas. Unlike apoptosis necrosis, Disulfidptosis driven abnormal accumulation intracellular disulfide bonds, leading protein misfolding cytoskeletal collapse, cancer cells metabolic dysregulation. We aim explore how respond identify potential analyzing gliomas at single-cell level using RNA sequencing (scRNA-seq). scRNA-seq data from patients were analyzed uncover differences ferroptosis-related pathways, iron metabolism lipid peroxidation. Cellular subpopulations within profiled assess their sensitivity underlying mechanisms. Survival analysis was conducted evaluate clinical relevance Disulfidptosis-related gene expression. Multiple exhibit varying sensitivities influenced properties. Dysregulated antioxidant mechanisms identified as key factors impacting sensitivity. Glioma microenvironment signaling pathways also play regulating Disulfidptosis. These findings suggest that activating may provide novel strategies overcome offers insights into progression highlights its target. By leveraging data, research uncovers tumor identifies specific populations resistant pave way personalized improve outcomes patients.

Language: Английский

Citations

0