De novo Biosynthesis of Caffeic Acid and Chlorogenic Acid in Escherichia coli via Enzyme Engineering and Pathway Engineering

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Caffeic acid (CA) and chlorogenic (CGA) have diverse health benefits, including hemostatic, antioxidant, antiinflammatory, highlighting their potential for medical applications. However, the absence of high-performance production strains increases costs, limiting wider application. In this study, we engineered Escherichia coli de novo CA CGA. To improve production, a highly efficient mutant tyrosine ammonia-lyase from Rhodotorula taiwanensis (RtTALT415M/Y458F) was identified using genome mining protein engineering. By engineering biosynthetic pathway through deletion pheA tyrR, along with overexpression aroGfbr tyrAfbr, developed an E. strain, CA11, which produced 6.36 g/L yield 0.06 g/g glucose productivity 0.18 g/L/h. This represents highest titer reported microbial synthesis as sole carbon source in coli. Based on strain further CGA13, optimized replicons, promoters, ribosome-binding sites, 1.53 CGA fed-batch fermentation, its industrial-scale production.

Language: Английский

Ciprofol Ameliorates Myocardial Ischemia/Reperfusion Injury by Inhibiting Ferroptosis Through Upregulating HIF-1α

Drug Design Development and Therapy, Journal Year: 2024, Volume and Issue: Volume 18, P. 6115 - 6132

Published: Dec. 1, 2024

Ciprofol is a novel intravenous anesthetic that has been increasingly used in clinical anesthesia and sedation. Studies suggested ciprofol reduced oxidative stress inflammatory responses to alleviate cerebral ischemia/reperfusion (I/R) injury, but whether protects the heart against I/R injury mechanisms are unknown. Herein, we assessed effects of on ferroptosis during myocardial injury.

Language: Английский