Construction of the bromodomain-containing protein-associated prognostic model in triple-negative breast cancer DOI Creative Commons
Wei Chen, Yushuai Yu, Chenxi Wang

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 18, 2025

Bromodomain-containing protein (BRD) play a pivotal role in the development and progression of malignant tumours. This study aims to identify prognostic genes linked BRD-related (BRDRGs) patients with triple-negative breast cancer (TNBC) construct novel model. Data from TCGA-TNBC, GSE135565, GSE161529 were retrieved public databases. was used key cell types. The BRDRGs score TCGA-TNBC calculated using single-sample Gene Set Enrichment Analysis (ssGSEA). Differential expression analysis performed differentially expressed (DEGs): DEGs1 cells, DEGs2 between tumours controls DEGs3 high low subgroups TCGA-TNBC. Differentially (DE-BRDRGs) determined by overlapping DEGs1, DEGs3. Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis, protein-protein interaction (PPI) network conducted investigate active pathways molecular interactions. Prognostic selected through univariate Cox regression least absolute shrinkage selection operator (LASSO) analyses risk model calculate scores. TNBC samples classified into low-risk groups based on median score. Additionally, correlations clinical characteristics, (GSEA), immune pseudotime performed. A total 120 DE-BRDRGs identified 605 four types, 10,776 DEGs2, 4,497 GO revealed enriched terms such as 'apoptotic process,' 'immune response,' 'regulation cycle,' while 56 KEGG pathways, including 'MAPK signaling pathway,' associated DE-BRDRGs. comprising six (KRT6A, PGF, ABCA1, EDNRB, CTSD GJA4) constructed. nomogram independent factors also developed. Immune abundance significantly higher high-risk group. In both groups, TP53 exhibited highest mutation frequency. KRT6A, went decreased progressively pseudotime. for developed validated, providing fresh insights relationship BRD TNBC.

Language: Английский

Construction of the bromodomain-containing protein-associated prognostic model in triple-negative breast cancer DOI Creative Commons
Wei Chen, Yushuai Yu, Chenxi Wang

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 18, 2025

Bromodomain-containing protein (BRD) play a pivotal role in the development and progression of malignant tumours. This study aims to identify prognostic genes linked BRD-related (BRDRGs) patients with triple-negative breast cancer (TNBC) construct novel model. Data from TCGA-TNBC, GSE135565, GSE161529 were retrieved public databases. was used key cell types. The BRDRGs score TCGA-TNBC calculated using single-sample Gene Set Enrichment Analysis (ssGSEA). Differential expression analysis performed differentially expressed (DEGs): DEGs1 cells, DEGs2 between tumours controls DEGs3 high low subgroups TCGA-TNBC. Differentially (DE-BRDRGs) determined by overlapping DEGs1, DEGs3. Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis, protein-protein interaction (PPI) network conducted investigate active pathways molecular interactions. Prognostic selected through univariate Cox regression least absolute shrinkage selection operator (LASSO) analyses risk model calculate scores. TNBC samples classified into low-risk groups based on median score. Additionally, correlations clinical characteristics, (GSEA), immune pseudotime performed. A total 120 DE-BRDRGs identified 605 four types, 10,776 DEGs2, 4,497 GO revealed enriched terms such as 'apoptotic process,' 'immune response,' 'regulation cycle,' while 56 KEGG pathways, including 'MAPK signaling pathway,' associated DE-BRDRGs. comprising six (KRT6A, PGF, ABCA1, EDNRB, CTSD GJA4) constructed. nomogram independent factors also developed. Immune abundance significantly higher high-risk group. In both groups, TP53 exhibited highest mutation frequency. KRT6A, went decreased progressively pseudotime. for developed validated, providing fresh insights relationship BRD TNBC.

Language: Английский

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