Knockdown of VDAC1 Promotes Ferroptosis in Diffuse Large B‐Cell Lymphoma DOI Open Access

Chuanming Lin,

Liuyan Xin,

Shuiling Xie

et al.

Hematological Oncology, Journal Year: 2025, Volume and Issue: 43(2)

Published: Feb. 21, 2025

ABSTRACT Diffuse large B‐cell lymphoma (DLBCL) is a prevalent subtype of non‐Hodgkin's (NHL). Ferroptosis novel form cell death involved in multiple tumor development. However, the relationship between ferroptosis‐related genes and DLBCL has not been extensively studied. The GSE95290 dataset was downloaded from Gene Expression Omnibus (GEO) database merged with associated ferroptosis to screen differentially expressed (DEGs). Hub were identified by constructing protein‐protein interaction (PPI) network. messenger RNA (mRNA) expressions hub subsequently detected vitro using reverse transcriptase quantitative polymerase chain reaction (RT‐qPCR). impact voltage dependent anion channel 1 (VDAC1) on proliferation, apoptosis, evaluated Cell Counting Kit‐8, flow cytometry, relevant assays, respectively. Six highly identified, all which could be used as diagnostic biomarkers for DLBCL. In studies revealed that suppressing VDAC1 expression inhibited proliferation promoted apoptosis. Furthermore, knockdown cells xenograft models, resulting elevated levels malondialdehyde (MDA) iron increased protein Acyl‐CoA synthetase long‐chain family 4 (ACSL4) cyclooxygenase‐2 (COX2). Conversely, glutathione (GSH) superoxide dismutase (SOD) reduced, accompanied decreased peroxidase (GPX4) ferritin heavy chain1 (FTH1). induces DLBCL, provides new insights into pathogenic mechanisms

Language: Английский

Knockdown of VDAC1 Promotes Ferroptosis in Diffuse Large B‐Cell Lymphoma DOI Open Access

Chuanming Lin,

Liuyan Xin,

Shuiling Xie

et al.

Hematological Oncology, Journal Year: 2025, Volume and Issue: 43(2)

Published: Feb. 21, 2025

ABSTRACT Diffuse large B‐cell lymphoma (DLBCL) is a prevalent subtype of non‐Hodgkin's (NHL). Ferroptosis novel form cell death involved in multiple tumor development. However, the relationship between ferroptosis‐related genes and DLBCL has not been extensively studied. The GSE95290 dataset was downloaded from Gene Expression Omnibus (GEO) database merged with associated ferroptosis to screen differentially expressed (DEGs). Hub were identified by constructing protein‐protein interaction (PPI) network. messenger RNA (mRNA) expressions hub subsequently detected vitro using reverse transcriptase quantitative polymerase chain reaction (RT‐qPCR). impact voltage dependent anion channel 1 (VDAC1) on proliferation, apoptosis, evaluated Cell Counting Kit‐8, flow cytometry, relevant assays, respectively. Six highly identified, all which could be used as diagnostic biomarkers for DLBCL. In studies revealed that suppressing VDAC1 expression inhibited proliferation promoted apoptosis. Furthermore, knockdown cells xenograft models, resulting elevated levels malondialdehyde (MDA) iron increased protein Acyl‐CoA synthetase long‐chain family 4 (ACSL4) cyclooxygenase‐2 (COX2). Conversely, glutathione (GSH) superoxide dismutase (SOD) reduced, accompanied decreased peroxidase (GPX4) ferritin heavy chain1 (FTH1). induces DLBCL, provides new insights into pathogenic mechanisms

Language: Английский

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