IntechOpen eBooks,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Nov. 3, 2023
Electrophoresis
is
a
versatile
technique
that
allows
for
the
separation
of
molecules
based
on
their
size
and
electrical
charge.
In
field
dentistry,
electrophoresis
widely
used
in
various
applications,
including
analysis
dental
materials
proteins
associated
with
diseases
oral
cavity.
Through
electrophoresis,
it
possible
to
evaluate
distribution
filler
particles
within
resin
matrices,
providing
valuable
information
mechanical
properties
durability
composite
restorations.
Furthermore,
this
has
significantly
contributed
study
implicated
diseases,
such
as
caries
periodontitis.
By
effectively
identifying
separating
these
proteins,
researchers
gain
deeper
understanding
mechanisms
underlying
conditions,
facilitating
development
innovative
therapeutic
strategies.
Overall,
application
dentistry
emerged
an
indispensable
tool
comprehensive
characterization
diseases.
Journal of Interferon & Cytokine Research,
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 18, 2024
The
goal
of
the
current
study
was
to
assess
levels
salivary
interleukin
(IL)-38,
IL-1β,
and
IL-10
in
various
periodontal
clinical
conditions.
In
total,
60
(20
healthy,
20
gingivitis,
stage
II–III,
grade
A–B
periodontitis)
subjects
were
included
study.
Demographic
parameters
recorded.
Samples
examined
for
IL-38,
by
means
enzyme-linked
immunosorbent
assay.
Results
demonstrated
that
periodontitis
group
had
significantly
lower
IL-38
(P
<
0.05)
than
healthy
group.
Salivary
did
not
differ
between
groups
>
0.05).
IL-1β
gingivitis
0.001)
0.01)
higher
those
present
indicated
level
is
decreased
disease.
results
suggested
a
possible
role
inflammation
process.
Clarifying
mechanisms
inflammatory
process
may
contribute
development
novel
treatment
strategies
diseases.
Dentistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 25, 2024
Periodontal
diseases
represent
a
spectrum
of
gingival
disorders
with
multifaceted
etiologies.
Identifying
and
utilizing
biomarkers
in
these
conditions
are
essential
for
early
detection,
risk
stratification,
personalized
therapeutic
interventions.
This
chapter
provides
comprehensive
overview
biomarker
research
diseases,
emphasizing
clinical
applications,
detection
methods,
the
potential
saliva
crevicular
fluid
as
diagnostic
vehicles.
We
also
delve
into
emerging
areas
such
microbiome-associated,
epigenetic,
metagenomic
biomarkers.
The
underscores
challenges
associated
validation,
promise
multi-marker
panels
improved
accuracy,
longitudinal
studies
to
predict
disease
progression.
As
point-of-care
technologies
wearables
pave
way
future
diagnostics,
innovative
solutions
like
biosensors
micro-electro-mechanical
systems
(MEMS)
highlighted.
encapsulates
importance
advancing
discovery
its
pivotal
role
reshaping
management.
Journal of Indian Society of Periodontology,
Journal Year:
2023,
Volume and Issue:
27(4), P. 339 - 340
Published: July 1, 2023
With
the
increase
in
research
on
biomarkers,
their
role
existence,
severity
and
pathogenicity
of
periodontal
disease
is
becoming
much
more
clearer
with
number
studies
being
published
these
biomarkers.
Biomarkers
are
objective
quantifiable
determinants
normal
biological
or
pathogenic
processes
can
be
measured
accurately
reproducibly.[1]
The
use
biomarkers
diagnosis
management
diseases
could
add
immense
value
to
our
gold
standard
clinical
measurements
which
current
based.
help
overcoming
limitations
various
indices
used
diagnosis.
have
been
classified
into
five
groups
based
function
diverse
stages:
1)
Antecedent
that
identifying
risk
developing
a
disease,
2)
Screening
monitoring
subclinical
stage
3)
Diagnostic
diagnose,
4)
Staging
identify
severity,
5)
Prognostic
predict
course,
duration,
treatment
efficacy
outcome
recurrence.[1,2]
play
critical
prevention,
disease.
studied
widely
detect
exposure
by
studying
pathogen
its
virulence
factors
(biomarkers
exposure);
host
response
as
result
infectious
agent
measuring
proinflammatory
cytokines
other
proteins
effect);
recognise
recognize
susceptible
individuals
e.g.,
genetic
susceptibility).[1,2]
Endpoints
trials
either
non-clinical.
non-clinical
endpoints.
Poor
selection
endpoints
makes
elucidation
application
findings,
challenging
impossible.[3]
problem
is,
despite
plethora
evidence
generated
innumerable
results
do
not
find
way
usage
chairside
assays.
There
deficiency
confirmatory
biomarker
for
it
chair
side
assays
practice.
This
attributed
heterogenicity
studies,
basis
definitions
endpoint
differences
subject
recruitment
disparities
sample
management.
Disparities
collection,
storage
transportation
methods,
variations
biochemical
microbiological
techniques
study
samples.[4]
Pavloum
et
al.
mentioned
four
steps
research:
(a)
Preclinical
exploratory
likely
Usually
molecules
having
capacity
differentiate
between
small
samples
from
healthy
diseased
identified;
(b)
Development
validation
an
assay
measure
interest
independent
sample;
(c)
Retrospective
targeted
large
variability
population
ensure
statistical
precision;
(d)
continue
show
differentiate,
progress
next
phase
prospective
trials.[5]
development
would
require
multiple
multiphase
procedure.
A
seven
point
checklist
especially
has
suggested
so
heterogenicities
reduced
comparability
this
may
achievable.[4]
provides
researchers
indispensable
fundamentals
checkpoints,
pertinent
specific
research.[4]
talks
about
specificity
material,
clear
health
changes
observed
(being
studied),
population,
sampling
detection
methodology,
values
sensitivity
finally
interpretation.[4]
implementation
criteria,
might
become
possibility
near
future,
that,
wealth
information
available,
utilized
practice
leading
personalized
treatment.
These
guidelines
useful
streamlining
process
reduce
clutter
waste.
IntechOpen eBooks,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Nov. 3, 2023
Electrophoresis
is
a
versatile
technique
that
allows
for
the
separation
of
molecules
based
on
their
size
and
electrical
charge.
In
field
dentistry,
electrophoresis
widely
used
in
various
applications,
including
analysis
dental
materials
proteins
associated
with
diseases
oral
cavity.
Through
electrophoresis,
it
possible
to
evaluate
distribution
filler
particles
within
resin
matrices,
providing
valuable
information
mechanical
properties
durability
composite
restorations.
Furthermore,
this
has
significantly
contributed
study
implicated
diseases,
such
as
caries
periodontitis.
By
effectively
identifying
separating
these
proteins,
researchers
gain
deeper
understanding
mechanisms
underlying
conditions,
facilitating
development
innovative
therapeutic
strategies.
Overall,
application
dentistry
emerged
an
indispensable
tool
comprehensive
characterization
diseases.