Effects of fixatives and storage duration on avian sperm morphology DOI Creative Commons
Gaute Grønstøl,

Marie Danielsen,

Emily R. A. Cramer

et al.

Journal of Ornithology, Journal Year: 2022, Volume and Issue: 164(1), P. 171 - 181

Published: Sept. 8, 2022

Abstract Sperm cells are variable both within and among species. To be able to accurately measure sperm understand their function, it is important that preserved in a manner maintains structural integrity. Formalin widely used fixative storage medium for cells, but few studies have examined the effect of fixation long-term on morphological Ethanol also common agent tissue samples, here we examine if formalin or ethanol alters cell size We found no significant effects process fresh fixed ethanol. Further, there were consistent length changes stored over period 227 days, three years. A comparison across 13–14 years time showed small reduction 0.93%. Furthermore, initially remained quite stable dry glass slides minimum six months (we mean 0.18% after 6 months). The proportion with head damage was, however, much higher samples than those formalin. Overall, 70% had acrosome versus only 3% Finding intact measuring length, therefore, required greater effort samples. Our findings indicate use from study morphometrics justified either fixative, although clearly preserves better.

Language: Английский

Sperm head morphology is associated with sperm swimming speed: A comparative study of songbirds using electron microscopy DOI
Hanna Nyborg Støstad, Arild Johnsen, Jan T. Lifjeld

et al.

Evolution, Journal Year: 2018, Volume and Issue: 72(9), P. 1918 - 1932

Published: July 17, 2018

Sperm exhibit extraordinary levels of morphological diversification across the animal kingdom. In songbirds, sperm have a helically shaped head incorporating distinct acrosomal membrane or "helical keel," form and extent which varies species. The functional significance this helical shape, however, remains unknown. Using scanning electron microscopy, we quantified inter‐ intraspecific variation in morphology 36 songbird species (Passeriformes: Passerida). phylogenetic comparative methods, investigated relationship between both swimming speed frequency extra‐pair young (EPY). We found that whose had relatively more pronounced (i.e., long acrosome, short nucleus, wide membrane, waveform along "core") faster‐swimming sperm. no evidence interspecific EPY, although did find among‐ within‐male traits were negatively correlated with EPY. Applying principles fluid mechanics, discuss how may influence speed, suggest further studies considering aspects beyond length are needed to improve our understanding structure‐function relationships.

Language: Английский

Citations

41

Longer Sperm Swim More Slowly in the Canary Islands Chiffchaff DOI Creative Commons
Emily R. A. Cramer,

Eduardo García-del-Rey,

Lars Erik Johannessen

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(6), P. 1358 - 1358

Published: May 31, 2021

Sperm swimming performance affects male fertilization success, particularly in species with high sperm competition. Understanding how morphology impacts is therefore important. speed hypothesized to increase total length, relative flagellum length (with the generating forward thrust), and midpiece (as contains mitochondria). We tested these hypotheses for divergence traits five island populations of Canary Islands chiffchaff (Phylloscopus canariensis). confirmed incipient mitochondrial DNA differentiation between Gran Canaria other islands. correlated negatively did not correlate (for only). The proportion motile cells increased on only. was similar across thus add a growing number studies passerine birds that do support morphology–swimming hypotheses. suggest mechanics are sufficiently different from mammalian predictions hydrodynamic models should no longer be applied this taxon. While both likely under selection passerines, relationship them requires further elucidation.

Language: Английский

Citations

13

Positive correlations between pre‐ and post‐copulatory sexual traits in warblers DOI
K. Supriya, Trevor D. Price, Melissah Rowe

et al.

Journal of Avian Biology, Journal Year: 2018, Volume and Issue: 49(5)

Published: March 10, 2018

Theoretical models predict that investment in pre‐copulatory and post‐copulatory sexually selected traits should trade‐off. At the macroevolutionary scale, majority of studies to date have focused on male weaponry as target sexual selection, but trade‐off equally apply used attract females, such bird song plumage. We studied Old World leaf warblers (Phylloscopidae), a group socially monogamous songbirds experience relatively high levels sperm competition. examined relationships between duration number elements with length across 21 species, same variables combined testes mass subset these species (n = 10). Across mass/sperm were generally positively correlated, albeit not statistically significantly so or borderline significance. In contrast theory, we found no evidence for negative associations pre‐ traits. argue this is consequence males some investing more into overall fertilization success (i.e. sum selection) than other achieved through both mate attraction

Language: Английский

Citations

7

Weak geographical structure in sperm morphology across the range of two willow warbler Phylloscopus trochilus subspecies in Scandinavia DOI
Hanna Nyborg Støstad, Silje L. Rekdal, Oddmund Kleven

et al.

Journal of Avian Biology, Journal Year: 2016, Volume and Issue: 47(5), P. 731 - 741

Published: March 12, 2016

Sperm morphology is highly diversified among species and at higher taxonomic levels. In birds, there also increasing evidence of geographical differentiation in sperm traits within species, especially those with strong competition. Geographical divergences might imply the formation a reproductive barrier speciation process. Here we study variation willow warblers Phylloscopus trochilus context Scandinavia, across range two subspecies that are differentiated certain genetic markers, migratory routes. We describe patterns genotypes (two previously described single‐nucleotide polymorphism (SNP) markers four polymorphic microsatellites); wing length, tarsus length body mass; 330 male sampled 33 localities Norway (58°N–69°N). Birds were on average larger longer‐winged north (spp. acredula ) than south ), showed sigmoid change SNP allele frequencies around 65°N. found no structuring microsatellites. There was Norway, except heads longer south. head associated for southern alleles, midpiece weakly one markers. Similar among‐male variances total sampling sites indicate uniform levels competition Norway. conclude remains rather undifferentiated trait between warbler which consistent pattern shallow divergence. This indicates not maintaining narrow hybrid zone.

Language: Английский

Citations

4

Effects of fixatives and storage duration on avian sperm morphology DOI Creative Commons
Gaute Grønstøl,

Marie Danielsen,

Emily R. A. Cramer

et al.

Journal of Ornithology, Journal Year: 2022, Volume and Issue: 164(1), P. 171 - 181

Published: Sept. 8, 2022

Abstract Sperm cells are variable both within and among species. To be able to accurately measure sperm understand their function, it is important that preserved in a manner maintains structural integrity. Formalin widely used fixative storage medium for cells, but few studies have examined the effect of fixation long-term on morphological Ethanol also common agent tissue samples, here we examine if formalin or ethanol alters cell size We found no significant effects process fresh fixed ethanol. Further, there were consistent length changes stored over period 227 days, three years. A comparison across 13–14 years time showed small reduction 0.93%. Furthermore, initially remained quite stable dry glass slides minimum six months (we mean 0.18% after 6 months). The proportion with head damage was, however, much higher samples than those formalin. Overall, 70% had acrosome versus only 3% Finding intact measuring length, therefore, required greater effort samples. Our findings indicate use from study morphometrics justified either fixative, although clearly preserves better.

Language: Английский

Citations

2