Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: 405, P. 111294 - 111294
Published: Oct. 30, 2024
Language: Английский
Poultry Science, Journal Year: 2024, Volume and Issue: 103(10), P. 104151 - 104151
Published: July 31, 2024
Infection by multidrug-resistant avian pathogenic Escherichia coli (APEC) in chickens always leads to the uselessness of antibiotics, highlighting need for alternative antibacterial agents. Sophora flavescens and Coptis chinensis have been a classical combination used together Traditional Chinese Medicine (TCM) formulas treat diseases with similar symptoms colibacillosis an extended period, but effect their active ingredients' on APEC infection remains unstudied. The objective this study was explore synergistic matrine berberine hydrochloride caused isolated APEC. In study, highly E. from liver diseased chicken farm suspected colibacillosis, it resistant multiple antibiotics. LD
Language: Английский
Citations
8
Experimental Eye Research, Journal Year: 2024, Volume and Issue: 240, P. 109830 - 109830
Published: Feb. 15, 2024
Language: Английский
Citations
2
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 18, 2024
Abstract Background H9N2 Avian Influenza Virus (AIV) poses a growing public health threat due to its rapid mutation rate and limited vaccine efficacy. Pulmonary Microvascular Endothelial Cells (PMVECs) play critical role as gateway for infection, highlighting the need alternative therapeutic strategies. This study examines antiviral potential of Oxymatrine (OMT), traditional Chinese medicine derivative, against AIV in PMVECs. Purpose The aim this is explore efficacy OMT modulating responses elucidate impact on TLR3 signaling pathway PMVECs infected with AIV. Study Design Methods: Using an array vitro assays such TCID50, CCK-8, RT-qPCR, ELISA, Western blot, evaluated viral infectivity, cell viability, gene protein expression levels, key cytokine levels Additionally, RNAi technology was employed silence genes further understand mechanisms involved. Results displayed dose-dependent inhibitory effect vital proteins PKR Mx1 modulated Type I interferons cytokines including IFN-α, IFN-β, IL-6, TNF-α. It significantly impacted pathways, affecting downstream components NF-κB IRF-3. silencing studies indicated that OMT's not solely dependent pathway. Conclusion Our findings reveal exhibits dual-action mechanism by inhibiting immune PMVECs, primarily through These results lay promising foundation development
Language: Английский
Citations
1
Molecules, Journal Year: 2024, Volume and Issue: 29(9), P. 1945 - 1945
Published: April 24, 2024
In our research, we explored a natural substance called Oxymatrine, found in traditional Chinese medicinal plant, to fight against common bird flu virus known as H9N2. This not only affects birds but can also pose threat human health. We focused on how this compound help stopping the from spreading cells that line lungs of and potentially humans. Our findings show Oxymatrine both directly block boost body’s immune response it. dual-action mechanism is particularly interesting because it indicates might be useful tool developing new ways prevent treat type flu. Understanding works H9N2 could lead safer more combat viral infections animals humans, contributing health well-being society. The Avian Influenza Virus (AIV) persistent its rapid mutation rate limited efficacy vaccines, underscoring urgent need for innovative therapies. study investigated AIV antiviral properties (OMT), derived medicine, focusing interaction with pulmonary microvascular endothelial (PMVECs). Employing an array vitro assays, including 50% tissue culture infectious dose, Cell Counting Kit-8, reverse transcription-quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, Western blot, systematically elucidated multifaceted effects OMT. OMT dose-dependently inhibited critical proteins (PKR Mx1) modulated expression I interferons key cytokines (IFN-α, IFN-β, IL-6, TNF-α), thereby affecting TLR3 signaling downstream elements (NF-κB IRF-3). OMT’s extended beyond TLR3-mediated responses, suggesting potential versatile agent. contributes growing body research use compounds agents underscores importance further investigating broader application combating infections.
Language: Английский
Citations
1
Aquaculture, Journal Year: 2023, Volume and Issue: 578, P. 740147 - 740147
Published: Sept. 22, 2023
Language: Английский
Citations
1
Published: Jan. 1, 2024
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Language: Английский
Citations
0
The Journal of Animal and Plant Sciences, Journal Year: 2024, Volume and Issue: 5, P. 1153 - 1165
Published: Aug. 22, 2024
Herbs are widely used in the treatment of a wide range illnesses humans and animals. They contain an array natural antibacterial compounds possess potent properties, making them promising source low-toxicity, high-efficiency alternative antimicrobial agents that less likely to develop drug resistance. In this experiment, total phenols flavonoids, free radical scavenging ability extracts derived from seven traditional Chinese herbs, including walnut (Juglans regia) green husk, Cistanche herba, Capparis spinosa, Eclipta prostrata, Sophora flavescens, Scutellaria barbata, Sargentodoxa cuneata, were investigated. Furthermore, properties against Staphylococcus aureus, Escherichia coli, Salmonella enteritidis assessed. The results showed concentrations flavonoids ranged 5.45 226.89 mg gallic acid equivalent (GAE)/mL 7.34 428.04 rutin (RE)/mL, respectively. S. cuneata extract exhibited highest contents which significantly higher than those other herbs (P < 0.05). 2,2-diphenyl-1-picrylhydrazyl (DPPH) rates 28.25% 85.33%. E. prostrata antioxidant activity as compared Extract husk possessed best aureus with inhibition zone diameters 13.05 8.59 mm, respectively, greater herbal For barbata displayed diameter 9.83 mm. Based on findings current study, it is concluded favorable capacity have potential serve alternatives for antibiotics. Keywords: Herbs, phenols, activity,
Language: Английский
Citations
0
Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: 405, P. 111294 - 111294
Published: Oct. 30, 2024
Language: Английский
Citations
0