HSFA1a modulates plant heat stress responses and alters the 3D chromatin organization of enhancer-promoter interactions

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 28, 2023

Abstract The complex and dynamic three-dimensional organization of chromatin within the nucleus makes understanding control gene expression challenging, but also opens up possible ways to epigenetically modulate expression. Because plants are sessile, they evolved sophisticated rapidly in response environmental stress, that thought be coordinated by changes conformation mediate specific cellular physiological responses. However, what extent how stress induces reorganization remains poorly understood. Here, we comprehensively investigated genome-wide associated with transcriptional reprogramming heat tomato. Our data show rapid architecture, leading transient formation promoter-enhancer contacts, likely driving heat-stress responsive genes. Furthermore, demonstrate spatial requires HSFA1a, a transcription factor (TF) essential for tolerance In light our findings, propose TFs play key role controlling responses through 3D reconfiguration contacts.

Language: Английский

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Genetic and molecular exploration of maize environmental stress resilience: Toward sustainable agriculture

Molecular Plant, Journal Year: 2023, Volume and Issue: 16(10), P. 1496 - 1517

Published: July 18, 2023

Language: Английский

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Transcriptional Stress Memory and Transgenerational Inheritance of Drought Tolerance in Plants

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 12918 - 12918

Published: Oct. 26, 2022

Plants respond to drought stress by producing abscisic acid, a chemical messenger that regulates gene expression and thereby expedites various physiological cellular processes including the stomatal operation mitigate promote tolerance. To trigger or suppress transcription under conditions, surrounding chromatin architecture must be converted between repressive active state epigenetic remodeling, which is achieved dynamic interplay among DNA methylation, histone modifications, loop formation, non-coding RNA generation. can memorize status conditions enable them deal with recurrent stress. Furthermore, tolerance acquired during plant growth transmitted next The epigenetically modified architectures of memory genes stressful newly developed cells mitotic cell division, germline offspring overcoming restraints on meiosis. In mammalian cells, completely erased reset mechanism overcome this resetting meiosis transmit unclear. article, we review recent findings underlying transcriptional transgenerational inheritance in plants.

Language: Английский

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Mind the gap: Epigenetic regulation of chromatin accessibility in plants

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 194(4), P. 1998 - 2016

Published: Jan. 17, 2024

Abstract Chromatin plays a crucial role in genome compaction and is fundamental for regulating multiple nuclear processes. Nucleosomes, the basic building blocks of chromatin, are central these processes, determining chromatin accessibility by limiting access to DNA various proteins acting as important signaling hubs. The association histones with nucleosomes folding into higher-order structures strongly influenced variety epigenetic marks, including methylation, histone variants, post-translational modifications. Additionally, wide array chaperones ATP-dependent remodelers regulate aspects nucleosome biology, assembly, deposition, positioning. This review provides an overview recent advances our mechanistic understanding how organization regulated marks plants. Furthermore, we present current technologies profiling organization.

Language: Английский

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Epigenetics in the modern era of crop improvements

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Epigenetic mechanisms are integral to plant growth, development, and adaptation environmental stimuli. Over the past two decades, our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body knowledge on both locus-specific genome-wide patterns. Studies initially grounded in model Arabidopsis have been broadened encompass diverse array crop species, revealing multifaceted roles epigenetics physiological agronomic traits. With recent technological advancements, epigenetic regulations at single-cell level large-scale population emerging as new focuses. This review offers an in-depth synthesis regulations, detailing catalytic machinery functions. It delves into intricate interplay among various elements their collective influence modulation Furthermore, it examines breakthroughs technologies for modifications integration strategies improvement. The underscores transformative potential bolstering performance, advocating development efficient tools fully exploit agricultural benefits insights.

Language: Английский

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The TRIPLE PHD FINGERS proteins are required for SWI/SNF complex-mediated +1 nucleosome positioning and transcription start site determination in Arabidopsis

Nucleic Acids Research, Journal Year: 2022, Volume and Issue: 50(18), P. 10399 - 10417

Published: Oct. 3, 2022

Abstract Eukaryotes have evolved multiple ATP-dependent chromatin remodelers to shape the nucleosome landscape. We recently uncovered an evolutionarily conserved SWItch/Sucrose Non-Fermentable (SWI/SNF) remodeler complex in plants reminiscent of mammalian BAF subclass, which specifically incorporates MINUSCULE (MINU) catalytic subunits and TRIPLE PHD FINGERS (TPF) signature subunits. Here we report experimental evidence that establishes functional relevance TPF proteins for activity. Our results show depletion triggers similar pleiotropic phenotypes molecular defects those found minu mutants. Moreover, genomic location MINU2 as representative members this SWI/SNF their impact on positioning transcription. These analyses unravel binding thousands genes where it modulates position +1 nucleosome. targets tend produce 5′-shifted transcripts tpf mutants pointing participation alternative transcription start site usage. Interestingly, there is a remarkable correlation between shift 5′ transcript length change suggesting connection. In summary, study unravels function plant involved determination.

Language: Английский

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Histone modification and chromatin remodeling in plant response to pathogens

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 3, 2022

As sessile organisms, plants are constantly exposed to changing environments frequently under diverse stresses. Invasion by pathogens, including virus, bacterial and fungal infections, can severely impede plant growth development, causing important yield loss thus challenging food/feed security worldwide. During evolution, have adapted complex systems, coordinated global gene expression networks, defend against pathogen attacks. In recent years, growing evidences indicate that infections trigger local epigenetic changes reprogram the transcription of defense genes, which in turn helps fight pathogens. Here, we summarize up pathways mechanisms review depth current knowledge's about histone modifications chromatin-remodeling factors found regulation response biotic It is anticipated may be explorable design tools generate stress-resistant varieties.

Language: Английский

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BCL7A and BCL7B potentiate SWI/SNF-complex-mediated chromatin accessibility to regulate gene expression and vegetative phase transition in plants

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 31, 2024

Abstract Switch defective/sucrose non-fermentable (SWI/SNF) chromatin remodeling complexes are multi-subunit machineries that establish and maintain accessibility gene expression by regulating structure. However, how the activities of SWI/SNF regulated in eukaryotes remains elusive. B-cell lymphoma/leukemia protein 7 A/B/C (BCL7A/B/C) have been reported as subunits for decades animals recently plants; however, role BCL7 function undefined. Here, we identify a unique plant BCL7A BCL7B homologous potentiating genome-wide plants. BCL7A/B require catalytic ATPase BRAHMA (BRM) to assemble with signature BRM-Associated (BAS) genomic binding at subset target genes. Loss diminishes BAS-mediated without changing stability targeting BAS complex, highlighting specialized activity. We further show fine-tune activity generate accessible juvenility resetting region (JRR) microRNAs MIR156A/C juvenile identity maintenance. In summary, our work uncovers previously elusive multicellular provides insights into mechanisms whereby plants memorize through SWI/SNF-mediated control accessibility.

Language: Английский

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Redox regulation of chromatin remodelling in plants

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: 47(8), P. 2780 - 2792

Published: Feb. 4, 2024

Changes in the cellular redox balance that occur during plant responses to unfavourable environmental conditions significantly affect a myriad of redox-sensitive processes, including those impact on epigenetic state chromatin. Various factors, like histone modifying enzymes, chromatin remodelers, and DNA methyltransferases can be targeted by oxidative posttranslational modifications. As their combined action affects regulation gene expression, they form an integral part (a)biotic stress. Epigenetic changes triggered are intrinsically linked with primary metabolism supplies intermediates donors, such acetyl-CoA S-adenosyl-methionine, critical for decoration histones DNA. Here, we review recent advances our understanding remodelling, dynamics marks, interplay between control signalling within stress context.

Language: Английский

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Epigenetic regulations under plant stress: A cereals perspective

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: 220, P. 105688 - 105688

Published: Feb. 10, 2024

Language: Английский

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