Identification of loci conferring resistance to 4 foliar diseases of maize DOI Creative Commons
Yuting Qiu, Pragya Adhikari, Peter Balint‐Kurti

et al.

G3 Genes Genomes Genetics, Journal Year: 2023, Volume and Issue: 14(2)

Published: Dec. 5, 2023

Foliar diseases of maize are among the most important worldwide. This study focused on 4 major foliar maize: Goss's wilt, gray leaf spot, northern corn blight, and southern blight. QTL mapping for resistance to wilt was conducted in disease introgression line populations with Oh7B as common recurrent parent Ki3, NC262, NC304, NC344 donor parents. Mapping results were combined previous studies blight same populations. We (1) individual linkage analysis identify specific each population; (2) Mahalanobis distance putative multiple regions 3) joint across disease. identified 3 lines that resistant all diseases. mapped 13 QTLs an additional 6 using mapping. All had small effects, confirming is highly quantitative. report several potentially chromosomal bins associated including 1.02, 1.03, 3.04, 4.06, 4.08, 9.03. Together, these findings indicate distribution not random there locations genome confer Furthermore, bacterial fungal entirely distinct, we both fungi bacteria, well loci

Language: Английский

Introgression of ZmCPK39 in maize hybrids enhances resistance to gray leaf spot disease without compromising yield DOI
Mang Zhu, Song Huang, Jingwen Xu

et al.

Molecular Breeding, Journal Year: 2025, Volume and Issue: 45(3)

Published: Feb. 24, 2025

Language: Английский

Citations

2
Heat Stress and Plant–Biotic Interactions: Advances and Perspectives DOI Creative Commons
Rahul Mahadev Shelake, Sopan Ganpatrao Wagh, Akshay Milind Patil

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(15), P. 2022 - 2022

Published: July 23, 2024

Climate change presents numerous challenges for agriculture, including frequent events of plant abiotic stresses such as elevated temperatures that lead to heat stress (HS). As the primary driving factor climate change, HS threatens global food security and biodiversity. In recent years, have negatively impacted physiology, reducing plant's ability maintain disease resistance resulting in lower crop yields. Plants must adapt their priorities toward defense mechanisms tolerate challenging environments. Furthermore, selective breeding long-term domestication higher yields made varieties vulnerable multiple stressors, making them more susceptible events. Studies on predict concurrent biotic will become severe future, potentially occurring simultaneously or sequentially. While most studies focused singular effects systems examine how plants respond specific stresses, simultaneous occurrence pose a growing threat agricultural productivity. Few explored interactions between plant-biotic interactions. Here, we aim shed light physiological molecular (bacteria, fungi, oomycetes, nematodes, insect pests, pollinators, weedy species, parasitic plants), well combined impact growth We also advances designing developing various strategies address multi-stress scenarios related factors.

Language: Английский

Citations

14
Natural variation in the pattern‐triggered immunity response in plants: Investigations, implications and applications DOI Creative Commons
Asher I. Hudson,

Alexander Mullens,

Sarah R. Hind

et al.

Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(3)

Published: March 1, 2024

Abstract The pattern‐triggered immunity (PTI) response is triggered at the plant cell surface by recognition of microbe‐derived molecules known as microbe‐ or pathogen‐associated molecular patterns derived from compromised host cells called damage‐associated patterns. Membrane‐localized receptor proteins, pattern receptors, are responsible for this recognition. Although much machinery PTI conserved, natural variation exists within and across species with respect to components recognition, activation response, strength induced. This review describes what about variation. We discuss how in can be measured knowledge might utilized control disease developing varieties enhanced resistance.

Language: Английский

Citations

13
Using genome‐wide associations and host‐by‐pathogen predictions to identify allelic interactions that control disease resistance DOI Creative Commons
Owen Hudson, Jeremy Brawner

The Plant Genome, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 24, 2025

Abstract Characterizing the molecular mechanisms underlying disease symptom expression has been used to improve human health and resistance in crops animal breeds. Quantitative trait loci genome‐wide association studies (GWAS) are widely identify genomic regions that involved progression. This study extends traditional GWAS significance tests of host pathogen marker main effects by utilizing dual‐genome reaction norm models evaluate importance host‐single nucleotide polymorphism (SNP) pathogen‐SNP interactions. Disease severity data from Fusarium ear rot (FER) on maize ( Zea mays L.) is demonstrate use both genomes selection for breeding identification interact across organisms impact FER development. Dual genome prediction improved heritability estimates, error variances, model accuracy while providing predictions host‐by‐pathogen interactions may be test SNP–SNP Independent populations identified significantly associated were using two different tests. Predictions dual with population structure or polygenic effects. As well, incorporating markers also included relationship matrices account structure. Subsequent evaluation protein–protein candidate genes near interacting SNPs provides a further silico method expedite genes.

Language: Английский

Citations

1
Integrated multi-omics investigation revealed the importance of phenylpropanoid metabolism in the defense response of Lilium regale Wilson to fusarium wilt DOI Creative Commons
Jie Deng,

Xiaoli Che,

Yue Gu

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(7)

Published: May 20, 2024

Abstract Lilies (genus Lilium) play a significant role in the global cut-flower industry, but they are highly susceptible to fusarium wilt caused by Fusarium oxysporum. However, Lilium regale, wild lily species, exhibits remarkable resistance F. To investigate quantitative of L. regale wilt, comprehensive multi-omics analysis was conducted. Upon inoculation with oxysporum, roots showed accumulation phenylpropane metabolites, including lignin precursors, flavonoids, and hydroxycinnamic acids. These findings were consistent upregulated expression phenylpropanoid biosynthesis-related genes encoding various enzymes, as revealed transcriptomics proteomics analyses. Furthermore, metabolomics data demonstrated differential activation monoterpenoid isoquinoline alkaloid biosynthesis. Colorimetry high-performance liquid chromatography analyses significantly higher levels total lignin, ferulic acid, phlorizin, quercetin contents scales compared ‘Siberia’ during oxysporum infection. phenylpropanes exhibited inhibitory effects on growth suppressed pathogenicity-related genes. Transcriptional regulatory network suggested that ethylene-responsive transcription factors (ERFs) may positively regulate Therefore, LrERF4 cloned transiently overexpressed wilt-susceptible Oriental hybrid ‘Siberia’. The overexpression resulted increased quercetin, while silencing through RNAi had opposite effect. In conclusion, metabolism plays crucial defense response against acting positive regulator

Language: Английский

Citations

7
Plant secondary metabolites-mediated plant defense against bacteria and fungi pathogens DOI
Wenjuan Yang, Lu Zhang, Yong Yang

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 217, P. 109224 - 109224

Published: Oct. 20, 2024

Language: Английский

Citations

7
Natural variations of maize ZmLecRK1 determine its interaction with ZmBAK1 and resistance patterns to multiple pathogens DOI Creative Commons

Zhen-Ju Li,

Junbin Chen, Chuang Liu

et al.

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(10), P. 1606 - 1623

Published: Sept. 20, 2024

Maize (Zea mays) is one of the most important crops in world, but its yield and quality are seriously affected by diverse diseases. Identifying broad-spectrum resistance genes crucial for developing effective strategies to control disease maize. In a genome-wide study maize, we identified G-type lectin receptor kinase ZmLecRK1, as new protein against Pythium aphanidermatum, causal pathogens stalk rot Genetic analysis showed that specific ZmLecRK1 allele can confer multiple The cell death phenotype mediated resistant variant requires co-receptor ZmBAK1. A naturally occurring A404S extracellular domain determines ZmLecRK1-ZmBAK1 interaction formation ZmLecRK1-related complexes. Interestingly, susceptible was found possess amino acid S404 present ancestral variants conserved among majority grass species, while with A404 only few maize inbred lines. Substitution S at position 404 ZmLecRK1-like proteins sorghum rice greatly enhances their ability induce death. Further transcriptomic reveals likely regulates gene expression related pathways wall organization or biogenesis response pathogen infection. Taken together, these results suggest binding affinity ZmBAK1, thereby enhancing active complexes defense Our work highlights biotechnological potential generating disease-resistant precisely modulating activity homologs through targeted base editing.

Language: Английский

Citations

5
Fine mapping of Ae-Ps4.5, a major locus for resistance to pathotype III of Aphanomyces euteiches in pea DOI

Clément Lavaud,

Angélique Lesné,

Théo Leprévost

et al.

Theoretical and Applied Genetics, Journal Year: 2024, Volume and Issue: 137(2)

Published: Feb. 1, 2024

Language: Английский

Citations

4
Transformation of Alternaria dauci demonstrates the involvement of two polyketide synthase genes in aldaulactone production and fungal pathogenicity DOI Creative Commons
Jacqueline Bernardino-Sgherri,

Elza Neau,

Joséphine Kocuiba

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Abstract Chemical warfare between the host and pathogen plays a crucial role in plant-necrotrophic interactions, but examples of its involvement quantitative disease resistance plants are poorly documented. In Daucus carota-Alternaria dauci pathosystem, novel toxin aldaulactone has been identified as key factor both fungal pathogenicity carrot’s partial to pathogen. Bioinformatic analyses have pinpointed secondary metabolism gene cluster that harbors two polyketide synthase genes, AdPKS7 AdPKS8, likely responsible for biosynthesis aldaulactone. Here, we present functional validation AdPKS8 genes production A. dauci. We generated knock-out mutants by replacing essential domains with hygromycin gene, marking first reported case genetic manipulation Following transformation, were analyzed via HPLC-UV assessed in planta. Aldaulactone was abolished all PKS mutants, which also exhibited significantly reduced on H1-susceptible carrot leaves. These findings confirm roles their contribution pathogenicity.

Language: Английский

Citations

0
It‘s Complicated: Why Are There So Few Commercially Successful Crop Varieties Engineered for Disease Resistance? DOI Creative Commons
Peter Balint‐Kurti, Qingli Liu

Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(3)

Published: March 1, 2025

ABSTRACT It is more than 40 years since the era of transgenic plants began and 30 after cloning first plant disease resistance genes. Despite extensive progress in our mechanistic understanding despite considerable sustained efforts commercial, nonprofit, academic governmental sectors, prospect commercially viable varieties carrying traits endowed by biotechnological approaches remains elusive. The cost complying with regulations governing release often cited as main reason for this lack success. While undeniably a substantial hurdle, other have been successfully commercialised. We argue that significant portion challenges producing crop engineered intrinsic to trait itself. In review, we briefly discuss used engineer resistance. further possible reasons why they not successful commercial context and, finally, try derive some lessons apply future efforts.

Language: Английский

Citations

0