Enemies at peace: Recent progress in Agrobacterium-mediated cereal transformation DOI Creative Commons
Shaoshuai Liu, Ke Wang, Shuaifeng Geng

et al.

The Crop Journal, Journal Year: 2024, Volume and Issue: 12(2), P. 321 - 329

Published: Jan. 26, 2024

Agrobacterium tumefaciens mediated plant transformation is a versatile tool for genetic engineering following its discovery nearly half century ago. Numerous modifications were made in application to increase efficiency, especially the recalcitrant major cereals plants. Recent breakthroughs efficiency continue role as mainstream technique CRISPR/Cas-based genome editing and gene stacking. These led higher frequency lower but more stable transgene copies with capability revolutionize modern agriculture. In this review, we provide brief overview of history Agrobacterium-mediated focus on most recent progress improve system both host recipient. A promising future biotechnology agriculture predicted.

Language: Английский

Improving plant C-to-G base editors with a cold-adapted glycosylase and TadA-8e variants DOI
Yingli Jiang, Xiao Zhi,

Zhaopeng Luo

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Rice Promoter Editing: An Efficient Genetic Improvement Strategy DOI Creative Commons
Bowen Wu,

Hangfei Luo,

Zhongbo Chen

et al.

Rice, Journal Year: 2024, Volume and Issue: 17(1)

Published: Aug. 30, 2024

Gene expression levels in rice (Oryza sativa L.) and other plant species are determined by the promoters, which directly control phenotypic characteristics. As essential components of genes, promoters regulate intensity, location, timing gene expression. They contain numerous regulatory elements serve as binding sites for proteins that modulate transcription, including transcription factors RNA polymerases. Genome editing can alter promoter sequences, thereby precisely modifying patterns specific ultimately affecting morphology, quality, resistance rice. This paper summarizes research on conducted recent years, focusing improvements yield, heading date, disease resistance. It is expected to inform application encourage further development crop genetic improvement with promote.

Language: Английский

Citations

3
Engineering a robust Cas12i3 variant‐mediated wheat genome editing system DOI Creative Commons
Wenxue Wang, Lei Yan, Jingying Li

et al.

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Wheat (Triticum aestivum L., 2n = 6x 42, AABBDD) is one of the most important food crops in world. CRISPR/Cas12i3, which belongs to type V-I Cas system, has attracted extensive attention recently due its smaller protein size and less-restricted canonical 'TTN' protospacer adjacent motif (PAM). However, relatively lower editing efficacy plants hexaploidy complex nature wheat, Cas12i3/Cas12i3-5M-mediated genome wheat not been documented yet. Here, we report engineering a robust Cas12i3-5M-mediated system through fusion T5 exonuclease (T5E) combination with an optimised crRNA expression strategy (Opt). We first showed that T5E, rather than ExoI, Cas12i3-5M increased gene efficiencies by up 1.34-fold 3.87-fold, compared Cas12i3 HEK293T cells, respectively. efficiency remains low wheat. then demonstrated Opt-T5E-Cas12i3-5M could enhance 1.20- 1.33-fold 4.05- 7.95-fold stable lines Opt-Cas12i3-5M Opt-Cas12i3, respectively, progressive 5'-end resection DNA strand at cleavage site deletion size. The enabled ranging from 60.71% 90.00% across four endogenous target genes three elite Chinese varieties. Together, developed enriches toolkits for either biological research or genetic improvement may be extended other polyploidy crop species.

Language: Английский

Citations

3
Modularly assembled multiplex prime editors for simultaneous editing of agronomically important genes in rice DOI Creative Commons
Ajay Gupta, Bo Liu, Saad Raza

et al.

Plant Communications, Journal Year: 2023, Volume and Issue: 5(2), P. 100741 - 100741

Published: Oct. 27, 2023

Prime editing (PE) technology enables precise alterations in the genetic code of a genome interest. PE offers great potential for identifying major agronomically important genes plants and them into superior variants, ideally targeting multiple loci simultaneously to realize collective effects edits. Here, we report development modular assembly-based multiplex system rice demonstrate its efficacy up four single transformation experiment. The duplex (DPE) achieved co-editing efficiency 46.1% T

Language: Английский

Citations

9
Enemies at peace: Recent progress in Agrobacterium-mediated cereal transformation DOI Creative Commons
Shaoshuai Liu, Ke Wang, Shuaifeng Geng

et al.

The Crop Journal, Journal Year: 2024, Volume and Issue: 12(2), P. 321 - 329

Published: Jan. 26, 2024

Agrobacterium tumefaciens mediated plant transformation is a versatile tool for genetic engineering following its discovery nearly half century ago. Numerous modifications were made in application to increase efficiency, especially the recalcitrant major cereals plants. Recent breakthroughs efficiency continue role as mainstream technique CRISPR/Cas-based genome editing and gene stacking. These led higher frequency lower but more stable transgene copies with capability revolutionize modern agriculture. In this review, we provide brief overview of history Agrobacterium-mediated focus on most recent progress improve system both host recipient. A promising future biotechnology agriculture predicted.

Language: Английский

Citations

3