Optimizing Sugarcane Clonal Propagation In Vitro by Using Calcium Ammonium Nitrate and Ammonium Sulfate DOI Creative Commons

Yuan-li Wu,

Faisal Mehdi,

Zhengying Cao

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(19), P. 2767 - 2767

Published: Oct. 2, 2024

To replace explosive nitrate-based chemicals in MS medium, this study developed a new, safer, and more cost-effective method using fertilizer-grade calcium ammonium nitrate sulfate. This approach replaces potassium nitrate, ensuring both safety cost efficiency for sugarcane propagation. Six local varieties—Zhongtang1 (ZT1), Zhongtang3 (ZT3), Zhongtang6 (ZT6), Guitang42 (GT42), Guitang44 (GT44), Guiliu 07150 (GT07150)—were used. In the control group (Ck), ions (NO3−) were 39.28 mM, (NH4+) 20.49 with 2:1 ratio. treatment groups, concentrations of included 1 (19.69 mM NO3− 10.3 NH4+), 2 (29.54 15.44 mM), 3 (39.38 20.59 4 (49.225 25.74 5 (59.07 30.89 6 (68.915 36.03 respectively, all same Fifty bottles per treatment, three replicates, used each plantlets treatment. After five subcultures, optimal ratio was determined by assessing morphological physiological parameters, nitrogen levels, SOD enzyme activity. The results indicated that mM) had best indicators. doses sulfate found treatments 4, as well control, no significant difference among them. However, 3, due to its lower dose, effective. improve practical production, it is recommended use concentration plant tissue culture plantlets.

Language: Английский

Different responses of the Lagerstroemia indica to varied supplies of ammonium and nitrate DOI
Yijing Wu,

Yan Xu,

Ziyi Liu

et al.

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 329, P. 113001 - 113001

Published: Feb. 15, 2024

Language: Английский

Citations

1

Endophytic Bacillus pumilus G5 Interacting with Silicon to Improve Drought Stress Resilience in Glycyrrhiza uralensis Fisch. by Modulating Nitrogen Absorption, Assimilation, and Metabolism Pathways DOI

Zhanchao Xu,

Qiuxian Bai,

Xueying Peng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(18), P. 10257 - 10270

Published: April 25, 2024

Drought stress has become the primary severe threat to global agriculture production, including medicinal plants. Plant growth-promoting bacteria (PGPB) and environmentally friendly element silicon (Si) have emerged as effective methods in alleviating drought various Here, effects of plant endophytic G5 interaction with Si on regulating nitrogen absorption, assimilation, metabolism pathways were investigated morphophysiological gene attributes Glycyrrhiza uralensis exposed drought. Results showed that G5+Si application improved absorption assimilation by increasing available content soil, further improving utilization efficiency. Then, triggered accumulation major adjustment substances proline, γ-aminobutyric acid, putrescine, chlorophyll, which played an important role contributing maintaining balance energy supply G. These findings will provide new ideas for combined PGPR both soil systems a habitat.

Language: Английский

Citations

1

Biological Characteristics of the Mycelium and Optimization of the Culture Medium for Phallus dongsun DOI Creative Commons
Chao Kang, Xuan Zheng,

W M Wang

et al.

Polish Journal of Microbiology, Journal Year: 2024, Volume and Issue: 73(2), P. 237 - 252

Published: June 1, 2024

Abstract This study aimed to elucidate the influence of various culture medium components, including carbon sources, nitrogen inorganic salts, suspension agents, and temperature, on mycelial growth characteristics Phallus dongsun . Employing single-factor experiments response surface methodology within glass Petri dishes, research identified that carrot powder, soybean ZnSO 4 notably enhanced proliferation aerial mycelium, significantly augmenting rate P. mycelium. The resultant mycelium was observed be dense, robust, fluffy in texture. In particular, markedly accelerated rate. Furthermore, xanthan gum found effectively modulate medium’s viscosity, ensuring a stable facilitating nutrient equilibrium. optimal cultivation temperature determined 25°C, with ceasing below 5°C perishing at temperatures exceeding 35°C. composition established as follows: wheat starch 5 g/l, powder 7.50 glucose 10 0.71 NH Cl 0.68 0.5 agar 20 g/l. Under these optimized conditions, exhibited rapid (1.04 ± 0.14 mm/day), characterized by thick, well-developed structure. investigation provides theoretical foundation for conservation, strain selection, breeding

Language: Английский

Citations

1

Boosting crop yield and nitrogen use efficiency: the hidden power of nitrogen-iron balance DOI Creative Commons
Jie Wu, Ying Song,

Guang-Yu Wan

et al.

New Crops, Journal Year: 2024, Volume and Issue: unknown, P. 100047 - 100047

Published: Sept. 1, 2024

Language: Английский

Citations

1

Optimizing Sugarcane Clonal Propagation In Vitro by Using Calcium Ammonium Nitrate and Ammonium Sulfate DOI Creative Commons

Yuan-li Wu,

Faisal Mehdi,

Zhengying Cao

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(19), P. 2767 - 2767

Published: Oct. 2, 2024

To replace explosive nitrate-based chemicals in MS medium, this study developed a new, safer, and more cost-effective method using fertilizer-grade calcium ammonium nitrate sulfate. This approach replaces potassium nitrate, ensuring both safety cost efficiency for sugarcane propagation. Six local varieties—Zhongtang1 (ZT1), Zhongtang3 (ZT3), Zhongtang6 (ZT6), Guitang42 (GT42), Guitang44 (GT44), Guiliu 07150 (GT07150)—were used. In the control group (Ck), ions (NO3−) were 39.28 mM, (NH4+) 20.49 with 2:1 ratio. treatment groups, concentrations of included 1 (19.69 mM NO3− 10.3 NH4+), 2 (29.54 15.44 mM), 3 (39.38 20.59 4 (49.225 25.74 5 (59.07 30.89 6 (68.915 36.03 respectively, all same Fifty bottles per treatment, three replicates, used each plantlets treatment. After five subcultures, optimal ratio was determined by assessing morphological physiological parameters, nitrogen levels, SOD enzyme activity. The results indicated that mM) had best indicators. doses sulfate found treatments 4, as well control, no significant difference among them. However, 3, due to its lower dose, effective. improve practical production, it is recommended use concentration plant tissue culture plantlets.

Language: Английский

Citations

1