Impact of Hormone on Growth and GA3 Regulation of Anthocyanin Biosynthesis in Suspension‐Culture Cells of Cyclocarya paliurus DOI Open Access

Chuanqing Pan,

Yuan Liu,

Daobang Tang

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

ABSTRACT Hormones are effective in regulating plant metabolism and, therefore, often used cell culture to increase the yield of target products. This study investigated effects hormones on growth and anthocyanin biosynthesis suspension‐cultured red Cyclocarya paliurus cells. Additionally, mechanism by which gibberellin induces was explored through multi‐omics integrated analysis assay dynamic changes signaling molecule concentration. The results showed that total content cells, when induced 1.0 mg L⁻¹ Gibberellin A 3 (GA ), experienced increases 1.92‐ 1.83‐fold, respectively. application exogenous GA activated synthesis transduction four molecules, is, nitric oxide (NO), hydrogen peroxide (H 2 O salicylic acid (SA), jasmonic (JA), cells altered expression patterns transcription factors. factors upregulated biosynthetic genes such as anthocyanin‐3‐O‐glucosyl transferase leucoanthocyanidin dioxygenase , while downregulated reductase flavonoid 3′,5′,‐hydroxylase pathway, ultimately leading a significant biosynthesis. research work establishes foundation for further role

Language: Английский

Phosphorylation of the strawberry MADS‐box CMB1 regulates ripening via the catabolism of abscisic acid DOI Creative Commons
Haoran Jia, Yanna Shi,

Zhengrong Dai

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Research on the ripening of fleshy fruits has relied techniques that measure transcriptional changes. How is linked to posttranslational modifications such as protein phosphorylation remains less studied. Here, we characterize MADS-box SEPALLATA 4 (SEP4) subfamily transcription factor FaCMB1, a key negative regulator controlling strawberry ripening, whose transcript and abundance decrease progressively with fruit development are repressed by abscisic acid (ABA). Transient RNAi or overexpression FaCMB1 significantly altered process affected content endogenous ABA ripening-related quality. Transcriptome sequencing (RNA-seq) analysis suggested manipulation expression levels FaASR (ABA-, stress-, ripening-induced), while can repress gene directly binding its promoter. Furthermore, inhibited activity FaCYP707A4, 8'-hydroxylase enzyme involved in catabolism. We show be phosphorylated kinase FaSTPK, Phos-tag assays indicated level increases during ripening. This affects ability promoter alleviates repression. In conclusion, elucidated feedback regulatory path involving FaCMB1-FaASR-FaCYP707A4-ABA. During process, an increase led levels, which, combined increased collectively impaired repression FaCMB1. Meanwhile, further leading accumulation

Language: Английский

Citations

0
Genome-wide analysis of the bHLH gene family in Spatholobus suberectus identifies SsbHLH112 as a regulator of flavonoid biosynthesis DOI Creative Commons

Shuangshuang Qin,

Ying Liang,

Yan Xie

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 6, 2025

Spatholobus suberectus Dunn (S. suberectus), a medicinal herb from the Leguminosae family, is widely utilized in traditional medicine. The dried stem of S. demonstrates variety pharmacological effects, primarily attributed to its rich content flavonoid compounds, such as catechin. bHLH gene family serves diverse functions plants, including regulating biosynthesis, yet specific function remains uncertain. To address this, sequenced genome was leveraged for an extensive genome-wide analysis family. This identified 156 SsbHLH genes, which were phylogenetically classified into 19 distinct subgroups. Of these, 153 genes mapped across 9 chromosomes, while 3 remained unlocalized. Furthermore, within identical subgroups displayed preserved exon-intron arrangements and motif patterns. Ka/Ks further revealed that most duplicated have undergone purifying selection. A subset 12 found be markedly associated with content, catechin, isoliquiritigenin, formononetin, genistein. Among SsbHLH112, strongly correlates catechin levels, shown elevate flavonoids accumulation when overexpressed Nicotiana benthamiana. overexpression also notably upregulated NbDFR NbLAR, consistent increased production. These results elucidate role transcription factors providing basis additional exploration suberectus.

Language: Английский

Citations

0
Quantitative Proteomics Analysis of ABA and GA3 Treated Malbec Berries Reveals Insights into H<sub>2</sub>O<sub>2</sub> Scavenging and Anthocyanin Dynamics DOI Open Access
Germán Murcia, Rodrigo Alonso, Federico Berli

et al.

Published: Aug. 12, 2024

Abscisic acid (ABA) and gibberellic (GA3) are regulators of fruit color sugar levels, the application these hormones is a common practice in commercial vineyards dedicated to production table grapes. However, effects exogenous ABA GA3 on wine cultivars remains unclear. We investigated impact applications Malbec grapevine berries across three developmental stages. found similar patterns berry total anthocyanin accumulation, induced by both treatments, closely associated with H2O2 levels. Quantitative proteomics revealed that positively modulated antioxidant defense proteins, mitigating H2O2. Consequently, proteins involved phenylpropanoid biosynthesis were downregulated, leading decreased content at almost ripe stage, particularly petunidin-3-G peonidin-3-G. Additionally, we noted increased levels non-anthocyanins E-viniferin quercetin treated berries, which may enhance scavenging stage. Using linear mixed-effects model, statistical significance fixed contents, demonstrating their roles accumulation. In conclusion, our findings suggest molecular mechanism influence content, ultimately impacting dynamics during ripening.

Language: Английский

Citations

2
Type-B response regulators MaRR_B9 and MaRR_B12 coordinate cytokinin-mediated negative regulation of anthocyanin biosynthesis in banana fruits DOI Creative Commons
Ruchika Rajput, Shivi Tyagi,

Kumar Anchal

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Anthocyanins are pigments responsible for vibrant plant colors and play vital roles in physiology. Comparing two banana varieties, grand naine (GN) red (RB), which display significant contrast anthocyanin pigmentation. Transcriptomic analysis of GN RB peel pulp tissue highlighted cytokinin-specific response regulators, MaRR_B9 MaRR_B12, as regulators biosynthesis. Through a combination physiological, molecular, biochemical analyses, we demonstrate that MaRR_B12 exert direct regulatory control over key structural genes biosynthesis, dihydroflavanol reductase (MaDFR) anthocyanidin synthase (MaANS). MaRRB_12 interact with the promoters MaDFR MaANS, repressing concerned vivo. Overexpression fruits leads to reduction content, particularly cyanidin derivative, along altered expression patterns MaANS. Thus, present study identifies novel biosynthesis provides further evidence cytokinin network modifies accumulation plants. In conclusion, our findings reveal new molecular targets, form MaRRs genetic manipulation content thereby development stress-resilient nutritionally enriched crop

Language: Английский

Citations

1
Transcription factor CcbHLH66 regulates mandarin fruit coloration via modulating the expression of chlorophyll degradation related genes CcRCCR and CcNYC DOI
Huali Wang,

Qian Liu,

Shu-fang Deng

et al.

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 218, P. 113188 - 113188

Published: Sept. 7, 2024

Language: Английский

Citations

1
The Softening of Persimmon Fruit Was Inhibited by Gibberellin via DkDELLA1/2 DOI
Wei Wu, Zhilong Bao, C Xiong

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

Texture is an important quality index reflecting a series of sensory evaluations fruit, and softening the main texture change most postharvest fruit. Persimmon fruit (Diospyros kaki) shows pattern decreasing firmness after harvest, leading to short duration sale excessive waste. We found that treatment with gibberellin (GA) could effectively inhibit persimmon but underlying mechanism unclear. In GA signal transduction pathway, DELLA protein key component involved in many growth development processes regulated by GA, while whether GA-regulated ripening not yet known. this research, two genes DkDELLA1/2 were enhance transactivation effect DkNAC9 on DkEGase1 promoter, thus participating GA. The results may provide new findings for regulatory network family.

Language: Английский

Citations

1
Quantitative Proteomics Analysis of ABA- and GA3-Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics DOI Creative Commons
Germán Murcia, Rodrigo Alonso, Federico Berli

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(17), P. 2366 - 2366

Published: Aug. 25, 2024

Abscisic acid (ABA) and gibberellic (GA

Language: Английский

Citations

0
Functional Analysis of Cytochrome b5 in Regulating Anthocyanin Biosynthesis in Malus domestica DOI Creative Commons
Fujun Zhang, Ning Ma, Haojian Li

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(10), P. 1075 - 1075

Published: Oct. 8, 2024

Cytochrome b5 (CB5), a small heme-binding protein, plays an important role in plant biotic and abiotic stress. Anthocyanin is critical determinant for fruit coloration, however, whether CB5 involved regulating anthocyanin biosynthesis has not yet been investigated apple (Malus domestica). In this study, we determined that MdCYB5, gene, was positive regulator fruit. We first found MdCYB5 showed high sequence structural similarity with Arabidopsis cytochrome isoform E (CB5E) at the protein level. Quantitative reverse transcription PCR (qRT-PCR) analysis responds to light signals. Subcellular localization localized cytoplasmin inthe epidermal cells of Nicotiana benthamiana leaves. Further investigation revealed overexpressing promoted both calli tissue-cultured seedlings. Furthermore, results transient expression assay accumulation coloration Taken together, study suggests regulatory effect on

Language: Английский

Citations

0
The MYC Gene RrbHLH105 Contributes to Salt Stress–Induced Geraniol in Rose by Regulating Trehalose‐6‐Phosphate Signalling DOI Open Access

Mingyue Bao,

Yongsheng Xu,

Guo Wei

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Rose (Rosa rugosa) is an important perfume plant, but its cultivation significantly constrained by salt stress. Terpenes represent the most abundant volatile aromatic compounds in roses, yet little known about how terpene metabolism responds to In this study, salt-treated rose petals presented significant accumulation of monoterpenes, including geraniol, due disruption jasmonic acid (JA) biosynthesis and signalling. Overexpression silencing analyses revealed a MYC transcription factor involved JA signalling (RrbHLH105) as repressor geraniol biosynthesis. RrbHLH105 was shown activate trehalose-6-phosphate synthase genes RrTPS5 RrTPS8 binding E-box (5'-CANNTG-3'). The increased content decreased overexpressing TPS5 or RrTPS8, along with high where both TPSs were cosilenced, indicate that trehalose plays role negative regulation via RrbHLH105-TPS module. summary, suppression stress leads excessive through inhibition RrbHLH105-mediated RrTPS-mediated petals. Additionally, study highlights emerging critical integrator crosstalk.

Language: Английский

Citations

0
The colorful clash: JA and GA signaling in apple anthocyanin biosynthesis through ubiquitination-dependent degradation DOI
Ying Duan, Zenglin Li

Plant Gene, Journal Year: 2024, Volume and Issue: 41, P. 100484 - 100484

Published: Dec. 11, 2024

Language: Английский

Citations

0