Soybean GmWRKY44 transcription factor activates SOC1 and LFY to promote flowering in Arabidopsis thaliana DOI
Yanchong Yu, Youju Huang,

Cuijing Pang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract WRKY transcription factors (TFs) play pivotal roles in regulating plant flowering; however, the molecular mechanism underlying flowering regulation by soybean (Glycine max) TFs remains elusive. In this study, we isolated GmWRKY44, a nuclear-localized Group IIc member exhibiting transcriptional activation capacity. GmWRKY44 displayed spatiotemporal specificity, with peak expression 30 d post-germination stems. GUS staining showed that expressed various tissues, such as roots, stems, leaves, sepals, stigmas, filaments, siliques and seedlings. promoter harbored 92 cis-elements associated phytohormone responses, light signaling, abiotic stress adaptation developmental regulation. Furthermore, overexpression of Arabidopsis led to an early-flowering phenotype, evidenced significant upregulation activators SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), LEAFY (LFY), APETALA1 (AP1) downregulation repressor FLOWERING LOCUS C (FLC). Subsequent analyses, including Y1H, EMSA, LUC assays, provided convincing evidences GmWRKY44 directly bound promoters SOC1 LFY, thereby elevating their expression. Genetic complementation assays further revealed OE44-1 soc1-2 lfy-2 hybrids exhibited later time than OE44-1 plants, indicating loss or LFY genetically arrested OE44-1. summary, study promoted upregulating thus addressing critical knowledge gap WRKYs on offering novel candidate gene for optimizing enhance yield across diverse agroecological zones.

Language: Английский

Genome-wide identification and expression analysis of the lipoxygenase gene family in sesame reveals regulatory networks in response to abiotic stress DOI

Tulsi,

Ishwar Patidar, Dinakara Rao Ampasala

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: Feb. 27, 2025

Language: Английский

Citations

0
Phylogenetic relationship of WRKY transcription factors in Solanum and potato genes in response to hormonal and biotic stresses DOI Creative Commons
Dongyang Jiang, Wenjuan Huang,

Jia Liu

et al.

Plant Signaling & Behavior, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 11, 2025

WRKY transcription factors are important regulators of plant responses to environmental stresses and hormone signaling. This study analyzes the gene family in Solanum tuberosum by examining phylogenetic relationships, expression profiles, their roles abiotic stress responses. Phylogenetic tree was constructed using 322 genes from four species: S. tuberosum, pennellii, pimpinellifolium, lycopersicum. The results revealed conserved expanded across these species. We then studied 75 SotuWRKY response salt, drought, heat stresses, treatments (IAA, ABA, BABA, GA3, BAP). Results showed that 19, 25, 29 were regulated under respectively. Several (e.g. SotuWRKY03 SotuWRKY24) also biotic like Phytophthora infestans infection treatments, indicating involvement defense mechanisms. A co-expression network based on gene-to-gene correlations, where SotuWRKY52 identified as a hub gene, positively regulating six negatively four. These findings suggest potato play key signaling, potentially enhancing resistance diseases. provides new insights into other

Language: Английский

Citations

0
Wheat Histone Deacetylase TaZIHD1 Interacts with Transcriptional Repressor TaZPAW1 to Negatively Regulate Zinc Uptake DOI
Xiaoyu Wang,

Wanzhen Chen,

Xiaofeng Liu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Zinc (Zn) is a chemical element essential to plant growth and development, soil Zn deficiency restricts agricultural production. Understanding the regulatory mechanism of uptake in bread wheat (Triticum aestivum) critical for improving production Zn-deficient regions. In this study, zinc-regulated transporters, iron-regulated transporter-like protein 10 (TaZIP10) was identified as functional transporter. The TaZIP10 exhibits Zn-transport activity yeast localizes plasma membrane. gene mainly expressed roots, its expression induced by deficiency. Silencing resulted decreased acquisition seedlings. Furthermore, we demonstrated that promoter-associated WRKY-type transcription factor 1 (TaZPAW1) directly binds promoter regions functions transcriptional repressor. Importantly, found TaZPAW1 interacts with histone deacetylase TaZIHD1 (TaZPAW1-interacting 1) recruits mediate deacetylation, which leads epigenetic suppression expression. addition, showed silencing or potentiated acquisition. These findings support concert negatively regulate presumably via epigenetically suppressing transporter gene.

Language: Английский

Citations

0
Toxicological Assessment of Bisphenol a in Capsicum Annuum L DOI

Z.Q. Zhang,

Lü Rong, Lei Li

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Soybean GmWRKY44 transcription factor activates SOC1 and LFY to promote flowering in Arabidopsis thaliana DOI
Yanchong Yu, Youju Huang,

Cuijing Pang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract WRKY transcription factors (TFs) play pivotal roles in regulating plant flowering; however, the molecular mechanism underlying flowering regulation by soybean (Glycine max) TFs remains elusive. In this study, we isolated GmWRKY44, a nuclear-localized Group IIc member exhibiting transcriptional activation capacity. GmWRKY44 displayed spatiotemporal specificity, with peak expression 30 d post-germination stems. GUS staining showed that expressed various tissues, such as roots, stems, leaves, sepals, stigmas, filaments, siliques and seedlings. promoter harbored 92 cis-elements associated phytohormone responses, light signaling, abiotic stress adaptation developmental regulation. Furthermore, overexpression of Arabidopsis led to an early-flowering phenotype, evidenced significant upregulation activators SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), LEAFY (LFY), APETALA1 (AP1) downregulation repressor FLOWERING LOCUS C (FLC). Subsequent analyses, including Y1H, EMSA, LUC assays, provided convincing evidences GmWRKY44 directly bound promoters SOC1 LFY, thereby elevating their expression. Genetic complementation assays further revealed OE44-1 soc1-2 lfy-2 hybrids exhibited later time than OE44-1 plants, indicating loss or LFY genetically arrested OE44-1. summary, study promoted upregulating thus addressing critical knowledge gap WRKYs on offering novel candidate gene for optimizing enhance yield across diverse agroecological zones.

Language: Английский

Citations

0